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comparison pyPRADA_1.2/tools/samtools-0.1.16/sam.c @ 0:acc2ca1a3ba4

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author siyuan
date Thu, 20 Feb 2014 00:44:58 -0500
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-1:000000000000 0:acc2ca1a3ba4
1 #include <string.h>
2 #include <unistd.h>
3 #include "faidx.h"
4 #include "sam.h"
5
6 #define TYPE_BAM 1
7 #define TYPE_READ 2
8
9 bam_header_t *bam_header_dup(const bam_header_t *h0)
10 {
11 bam_header_t *h;
12 int i;
13 h = bam_header_init();
14 *h = *h0;
15 h->hash = h->dict = h->rg2lib = 0;
16 h->text = (char*)calloc(h->l_text + 1, 1);
17 memcpy(h->text, h0->text, h->l_text);
18 h->target_len = (uint32_t*)calloc(h->n_targets, 4);
19 h->target_name = (char**)calloc(h->n_targets, sizeof(void*));
20 for (i = 0; i < h->n_targets; ++i) {
21 h->target_len[i] = h0->target_len[i];
22 h->target_name[i] = strdup(h0->target_name[i]);
23 }
24 return h;
25 }
26 static void append_header_text(bam_header_t *header, char* text, int len)
27 {
28 int x = header->l_text + 1;
29 int y = header->l_text + len + 1; // 1 byte null
30 if (text == 0) return;
31 kroundup32(x);
32 kroundup32(y);
33 if (x < y) header->text = (char*)realloc(header->text, y);
34 strncpy(header->text + header->l_text, text, len); // we cannot use strcpy() here.
35 header->l_text += len;
36 header->text[header->l_text] = 0;
37 }
38
39 samfile_t *samopen(const char *fn, const char *mode, const void *aux)
40 {
41 samfile_t *fp;
42 fp = (samfile_t*)calloc(1, sizeof(samfile_t));
43 if (strchr(mode, 'r')) { // read
44 fp->type |= TYPE_READ;
45 if (strchr(mode, 'b')) { // binary
46 fp->type |= TYPE_BAM;
47 fp->x.bam = strcmp(fn, "-")? bam_open(fn, "r") : bam_dopen(fileno(stdin), "r");
48 if (fp->x.bam == 0) goto open_err_ret;
49 fp->header = bam_header_read(fp->x.bam);
50 } else { // text
51 fp->x.tamr = sam_open(fn);
52 if (fp->x.tamr == 0) goto open_err_ret;
53 fp->header = sam_header_read(fp->x.tamr);
54 if (fp->header->n_targets == 0) { // no @SQ fields
55 if (aux) { // check if aux is present
56 bam_header_t *textheader = fp->header;
57 fp->header = sam_header_read2((const char*)aux);
58 if (fp->header == 0) goto open_err_ret;
59 append_header_text(fp->header, textheader->text, textheader->l_text);
60 bam_header_destroy(textheader);
61 }
62 if (fp->header->n_targets == 0 && bam_verbose >= 1)
63 fprintf(stderr, "[samopen] no @SQ lines in the header.\n");
64 } else if (bam_verbose >= 2) fprintf(stderr, "[samopen] SAM header is present: %d sequences.\n", fp->header->n_targets);
65 }
66 } else if (strchr(mode, 'w')) { // write
67 fp->header = bam_header_dup((const bam_header_t*)aux);
68 if (strchr(mode, 'b')) { // binary
69 char bmode[3];
70 int i, compress_level = -1;
71 for (i = 0; mode[i]; ++i) if (mode[i] >= '0' && mode[i] <= '9') break;
72 if (mode[i]) compress_level = mode[i] - '0';
73 if (strchr(mode, 'u')) compress_level = 0;
74 bmode[0] = 'w'; bmode[1] = compress_level < 0? 0 : compress_level + '0'; bmode[2] = 0;
75 fp->type |= TYPE_BAM;
76 fp->x.bam = strcmp(fn, "-")? bam_open(fn, bmode) : bam_dopen(fileno(stdout), bmode);
77 if (fp->x.bam == 0) goto open_err_ret;
78 bam_header_write(fp->x.bam, fp->header);
79 } else { // text
80 // open file
81 fp->x.tamw = strcmp(fn, "-")? fopen(fn, "w") : stdout;
82 if (fp->x.tamr == 0) goto open_err_ret;
83 if (strchr(mode, 'X')) fp->type |= BAM_OFSTR<<2;
84 else if (strchr(mode, 'x')) fp->type |= BAM_OFHEX<<2;
85 else fp->type |= BAM_OFDEC<<2;
86 // write header
87 if (strchr(mode, 'h')) {
88 int i;
89 bam_header_t *alt;
90 // parse the header text
91 alt = bam_header_init();
92 alt->l_text = fp->header->l_text; alt->text = fp->header->text;
93 sam_header_parse(alt);
94 alt->l_text = 0; alt->text = 0;
95 // check if there are @SQ lines in the header
96 fwrite(fp->header->text, 1, fp->header->l_text, fp->x.tamw); // FIXME: better to skip the trailing NULL
97 if (alt->n_targets) { // then write the header text without dumping ->target_{name,len}
98 if (alt->n_targets != fp->header->n_targets && bam_verbose >= 1)
99 fprintf(stderr, "[samopen] inconsistent number of target sequences. Output the text header.\n");
100 } else { // then dump ->target_{name,len}
101 for (i = 0; i < fp->header->n_targets; ++i)
102 fprintf(fp->x.tamw, "@SQ\tSN:%s\tLN:%d\n", fp->header->target_name[i], fp->header->target_len[i]);
103 }
104 bam_header_destroy(alt);
105 }
106 }
107 }
108 return fp;
109
110 open_err_ret:
111 free(fp);
112 return 0;
113 }
114
115 void samclose(samfile_t *fp)
116 {
117 if (fp == 0) return;
118 if (fp->header) bam_header_destroy(fp->header);
119 if (fp->type & TYPE_BAM) bam_close(fp->x.bam);
120 else if (fp->type & TYPE_READ) sam_close(fp->x.tamr);
121 else fclose(fp->x.tamw);
122 free(fp);
123 }
124
125 int samread(samfile_t *fp, bam1_t *b)
126 {
127 if (fp == 0 || !(fp->type & TYPE_READ)) return -1; // not open for reading
128 if (fp->type & TYPE_BAM) return bam_read1(fp->x.bam, b);
129 else return sam_read1(fp->x.tamr, fp->header, b);
130 }
131
132 int samwrite(samfile_t *fp, const bam1_t *b)
133 {
134 if (fp == 0 || (fp->type & TYPE_READ)) return -1; // not open for writing
135 if (fp->type & TYPE_BAM) return bam_write1(fp->x.bam, b);
136 else {
137 char *s = bam_format1_core(fp->header, b, fp->type>>2&3);
138 int l = strlen(s);
139 fputs(s, fp->x.tamw); fputc('\n', fp->x.tamw);
140 free(s);
141 return l + 1;
142 }
143 }
144
145 int sampileup(samfile_t *fp, int mask, bam_pileup_f func, void *func_data)
146 {
147 bam_plbuf_t *buf;
148 int ret;
149 bam1_t *b;
150 b = bam_init1();
151 buf = bam_plbuf_init(func, func_data);
152 bam_plbuf_set_mask(buf, mask);
153 while ((ret = samread(fp, b)) >= 0)
154 bam_plbuf_push(b, buf);
155 bam_plbuf_push(0, buf);
156 bam_plbuf_destroy(buf);
157 bam_destroy1(b);
158 return 0;
159 }
160
161 char *samfaipath(const char *fn_ref)
162 {
163 char *fn_list = 0;
164 if (fn_ref == 0) return 0;
165 fn_list = calloc(strlen(fn_ref) + 5, 1);
166 strcat(strcpy(fn_list, fn_ref), ".fai");
167 if (access(fn_list, R_OK) == -1) { // fn_list is unreadable
168 if (access(fn_ref, R_OK) == -1) {
169 fprintf(stderr, "[samfaipath] fail to read file %s.\n", fn_ref);
170 } else {
171 if (bam_verbose >= 3) fprintf(stderr, "[samfaipath] build FASTA index...\n");
172 if (fai_build(fn_ref) == -1) {
173 fprintf(stderr, "[samfaipath] fail to build FASTA index.\n");
174 free(fn_list); fn_list = 0;
175 }
176 }
177 }
178 return fn_list;
179 }