--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/NFR.xml	Fri Apr 12 05:28:43 2019 -0400
@@ -0,0 +1,51 @@
+<tool id="NFR" name="Nucleosome Free Regions" version="0.1">
+<description>: determine Nucleosome Free Regions from nucleR nucleosome calls</description>
+    <requirements>
+        <requirement type="binary">docker</requirement>
+    </requirements>
+    <command>
+<![CDATA[
+	docker run -v $__root_dir__/database/files:$__root_dir__/database/files -v /tmp:/tmp -u `id -u`:`id -g`  mmbirb/nucleosome-dynamics NFR --input $gff_file --output $output_gff_file --minwidth $minwidth --threshold $threshold  
+]]>
+  </command>
+  <inputs>
+    <param name="gff_file" type="data" format="gff" label="Nucleosome Calls (GFF)" help="Nucleosome calls in GFF format as obtained from nucleR."/>
+    <param name="minwidth" size="4" type="integer" value="110" label="Minimum Width" help="Minimum width for a linker fragment to be considered a nucleosome-free region." />  
+    <param name="threshold" size="4" type="integer" value="140" label="Maximum Width" help="Maximum width for a linker fragment to be considered a nucleosome-free region." />  
+</inputs>
+  <outputs>
+     <data format="gff" name="output_gff_file" label="NFR__${os.path.splitext(($gff_file.name.split('__'))[1])[0]}.gff" />
+  </outputs>
+  <tests>
+	  <test>
+		  <param name="gff_file" value="NR__cellcycleM_chrII.gff"/>
+		  <param name="minwidth" value="110" />
+		  <param name="threshold" value="400" />
+		  <output name="output_gff_file" file="NFR__cellcycleM_chrII.gff" />
+	  </test>
+  </tests>
+     <help>
+ .. image:: ${static_path}/images/NucleosomeDynamicsLogo.png
+    :height: 80
+    :width: 200
+
+-----
+
+Nucleosome Dynamics is a set of tools that take MNase-seq and ATAC-seq aligned reads and performs a serie of nucleosome-related analyses on them.
+
+.. class:: infomark
+
+Visit the  documentation of the original application for learning more about the accepted values and formats. http://mmb.irbbarcelona.org/NucleosomeDynamics/help/usage/nucleosome-dynamics
+   </help>
+   <citations>
+        <citation type="bibtex">
+@misc{github,
+  author = {Buitrago D},
+  year = {2019},
+  title = {Nucleosome Dynamics suite: containerized installation},
+  publisher = {GitHub},
+  journal = {GitHub repository},
+  url = {https://github.com/nucleosome-dynamics/nucleosome_dynamics},
+}</citation>
+ </citations>
+</tool>