Mercurial > repos > swebb > pycrac
diff pyCRAC/pyGTF2bedGraph.xml @ 0:19b20927172d draft
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author | swebb |
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date | Tue, 18 Jun 2013 09:11:00 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/pyCRAC/pyGTF2bedGraph.xml Tue Jun 18 09:11:00 2013 -0400 @@ -0,0 +1,162 @@ +<tool id="pyGTF2bedGraph" name="pyGTF2bedGraph"> + <description>converter</description> + <requirements> + <requirement type="package">pyCRAC</requirement> + </requirements> + <command interpreter="perl">pyGTF2bedGraph.pl --gtf $input --po $po --mo $mo + --chromfile $addchr.chr + -t $type + --count $count + $iclip + #if $addtrack.track == "--track": + --track + --name $addtrack.name + --description $addtrack.description + #if $addtrack.colorscheme.colorsel == "default": + --color $addtrack.colorscheme.color + #else: + -s '$addtrack.colorscheme.plus,$addtrack.colorscheme.minus' + #end if# + #end if# + --id $po.id + </command> + <version_command>/usr/local/bin/pyGTF2bedGraph.py --version</version_command> + <inputs> + <param name="input" type="data" format="gtf" label="GTF file --gtf"/> + <conditional name="addchr"> + <param name="chrfile" type="select" label="Choose Chromosome length file from"> + <option value="default" selected="true">Defaults</option> + <option value="other">History</option> + </param> + <when value="default"> + <param name="chr" type="select" label="Chromosome length file -c" help="This file should have two columns: first column is the names of the chromosomes, second column is length of the chromosomes. Use pyCrac utility pyCalculateChromosomeLengths to create."> + <options from_data_table="pycrac_chr"/> + </param> + </when> + <when value="other"> + <param format="tabular" name="chr" type="data" label="Chromosome length file -c" help="This file should have two columns: first column is the names of the chromosomes, second column is length of the chromosomes"/> + </when> + </conditional> + <param name="type" type="select" label="Choose type of data -t"> + <option value="reads" selected="true">Reads</option> + <option value="substitutions">Substitutions</option> + <option value="deletions">Deletions</option> + </param> + <param format="integer" name="count" type="integer" label="Count per feature --count " value="1" size="5" help="Takes the numbers in the 'score' column of the GTF file as the total number of reads for each position" > + <validator type="in_range" min="1" message="Please enter a value >= 1"/> + </param> + <param name="iclip" type="select" label="iCLIP mode --iCLIP"> + <option value="" selected="true">OFF</option> + <option value="--iCLIP">ON</option> + </param> + <conditional name="addtrack"> + <param name="track" type="select" label="Add UCSC track line to output"> + <option value="" selected="true">NO</option> + <option value="--track">YES</option> + </param> + <when value=""/> + <when value="--track"> + <param name="name" format="txt" type="text" value="User_supplied_track" size="80" label="Track name"/> + <param name="description" format="txt" type="text" value="User_supplied_track" size="80" label="Track description"/> + <conditional name="colorscheme"> + <param name="colorsel" type="select" label="Colouring scheme"> + <option value="default" selected="true">One Colour</option> + <option value="strand">By Strand</option> + </param> + <when value="default"> + <param name="color" type="select" label="Choose track colour"> + <option value="black" selected="true">Black</option> + <option value="red">Red</option> + <option value="blue">Blue</option> + <option value="green">Green</option> + <option value="purple">Purple</option> + </param> + </when> + <when value="strand"> + <param name="plus" type="select" label="Choose forward strand track colour"> + <option value="black" selected="true">Black</option> + <option value="red">Red</option> + <option value="blue">Blue</option> + <option value="green">Green</option> + <option value="purple">Purple</option> + </param> + <param name="minus" type="select" label="Choose minus strand track colour"> + <option value="black" selected="true">Black</option> + <option value="red">Red</option> + <option value="blue">Blue</option> + <option value="green">Green</option> + <option value="purple">Purple</option> + </param> + </when> + </conditional> + </when> + </conditional> + <param name="label" type="text" format="txt" size="30" value="pyGTF2bedGraph" label="Enter output file label -o" /> + </inputs> + <outputs> + <data name="po" format="bedgraph" label="${label.value}_plus_strand.bg"/> + <data name="mo" format="bedgraph" label="${label.value}_minus_strand.bg"/> + </outputs> + <help> + +.. class:: infomark + +**pyGTF2bedGraph** + +pyGTF2bedGraph is part of the pyCRAC_ package. Generates bedgraph files for each chromosome. An homage to bedtools genomecoverage. Takes a pyReadCounters GTF file as input file. Can also output bedGraph files for substitutions and deletions. + +.. _pyCRAC: http://sandergranneman.bio.ed.ac.uk/Granneman_Lab/pyCRAC_software.html + +------ + +**Parameter list** + +File input options:: + + --gtf=readdata.gtf + type the path to the gtf file data file. Be default it + expects data from the standard input + -o converted + provide a name for an output file. A file extension or + strand information is not necessary. + -c yeast.txt, --chromfile=yeast.txt + Location of the chromosome info file. This file should + have two columns: first column is the names of the + chromosomes, second column is length of the + chromosomes. Default is yeast + -t TYPE, --type=TYPE + this tool can generate bedGraph files for reads, + substitutions or deletions. Please use + 'reads','substitutions' or 'deletions' to indicate the + type of data. Default='reads' + --count + Takes the numbers in the 'score' column of the GTF + file as the total number of reads for each position. + Default is 1 for each interval. + --iCLIP + This turns on the iCLIP mode and the sgr reads or cDNA + files will report cross-linking site frequencies in + iCLIP data + -v, --verbose + to print status messages to a log file + +These options can be used to add a track line for the UCSC genome browser:: + + --track + Use this flag to add a UCSC genome browser track line + to the beginning of your file + -n NAME, --name=NAME + For the USCS track line: provide a track name. Default + = 'User_supplied_track' + -d DESCRIPTION, --description=DESCRIPTION + For the USCS track line: provide a track description. + Default = 'User_supplied_track' + --color=COLOR + select the track color. Default = black + -s STRANDS, --colorstrands=STRANDS + select the colors for each strand. Default = + 'red,blue' + + </help> +</tool> +