<tool id="snpEff_docker" name="SnpEff" version="3.6c (build 2014-05-20)">
	<description>Variant effect and annotation</description>
	<requirements>
		<container type="docker">takakoron/snpeff_3.6c</container>
	</requirements>
	<command interpreter="perl">/usr/src/myapp/snpEffWrapper.pl eff
-i $inputFormat 
-o $outputFormat 
-upDownStreamLen $udLength 
$canon
$hgvs
$lof
$motif
#if $statsFile:
  -stats $statsFile 
#end if
  $genomeVersion $input > $snpeff_output 
</command>
	<inputs>
		<param format="vcf,tabular,pileup,bed" name="input" type="data" label="Sequence changes (SNPs, MNPs, InDels)"/>

		<param name="inputFormat" type="select" label="Input format">
			<option value="vcf" selected="true">VCF</option>
			<option value="txt">Tabular</option>
			<option value="bed">BED</option>
		</param>

		<param name="outputFormat" type="select" label="Output format">
			<option value="vcf" selected="true">VCF</option>
			<option value="txt">Tabular</option>
			<option value="bed">BED</option>
			<option value="bedAnn">BED Annotations</option>
			<option value="gatk">VCF (GATK compatible)</option>
		</param>

		<param name="genomeVersion" type="select" label="Genome" help="If your annotation of interest is not listed, contact the P-GALAXY team (pipeline_dev@ddbj.nig.ac.jp)">
			<options from_file="snpEff_genomes.loc">
				<column name="name" index="1"/>
				<column name="value" index="0"/>
			</options>
		</param>

		<param name="udLength" type="select" label="Upstream / Downstream length">
			<option value="0">No upstream / downstream intervals (0 bases)</option>
			<option value="200">200 bases</option>
			<option value="500">500 bases</option>
			<option value="1000">1000 bases</option>
			<option value="2000">2000 bases</option>
			<option value="5000" selected="true">5000 bases</option>
			<option value="10000">10000 bases</option>
			<option value="20000">20000 bases</option>
		</param>

        <param name="canon" type="boolean" truevalue="-canon" falsevalue="" checked="false" label="Use only cannonical transcripts"/>
        <param name="hgvs" type="boolean" truevalue="-hgvs" falsevalue="" checked="false" label="Annotate using HGVS nomenclature"/>
        <param name="lof" type="boolean" truevalue="-lof" falsevalue="" checked="false" label="Annotate Loss of function (LOF) and Nonsense mediated decay (NMD)"/>
        <param name="motif" type="boolean" truevalue="-motif" falsevalue="" checked="false" label="Annotate transcription factor binding site motifs (only available for latest GRCh37)"/>
        <param name="generate_stats" type="boolean" truevalue="" falsevalue="-noStats" checked="true" label="Produce Summary Stats"/>
	</inputs>
	<outputs>
		<data format="tabular" name="snpeff_output" >
			<change_format>
				<when input="outputFormat" value="txt" format="tabular" />
				<when input="outputFormat" value="vcf" format="vcf" />
				<when input="outputFormat" value="bed" format="bed" />
				<when input="outputFormat" value="bedAnn" format="bed" />
			</change_format>
		</data>
                
		<data format="html" name="statsFile">
			<filter>generate_stats == True</filter>
		</data>
	</outputs>
        <stdio>
          <exit_code range=":-1"  level="fatal"   description="Error: Cannot open file" />
          <exit_code range="1:"  level="fatal"   description="Error" />
        </stdio>
        <tests>
            <test>
                <param name="input" ftype="vcf" value="vcf_homhet.vcf"/>
                <param name="inputFormat" value="vcf"/>
                <param name="outputFormat" value="vcf"/>
                <param name="genomeVersion" value="testCase"/>
                <param name="udLength" value="0"/>
                <output name="snpeff_output">
                    <assert_contents>
                        <!-- Check that an effect was added -->
                        <has_text text="EFF=NON_SYNONYMOUS_CODING" />
                    </assert_contents>
                </output>
                <output name="statsFile">
                    <assert_contents>
                        <!-- Check for a HTML header indicating that this was successful -->
                        <has_text text="SnpEff: Variant analysis" />
                    </assert_contents>
                </output>
            </test>
        </tests>
	<help>

This tool calculate the effect of variants (SNPs/MNPs/Insertions) and deletions.

For details about this tool, please go to http://snpEff.sourceforge.net

	</help>
</tool>