Mercurial > repos > tduigou > create_assembly_picklists
annotate create_assembly_picklists.xml @ 0:0b38f1504205 draft default tip
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
| author | tduigou |
|---|---|
| date | Thu, 16 Oct 2025 14:27:06 +0000 |
| parents | |
| children |
| rev | line source |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
parents:
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1 <tool id="create_assembly_picklists" name="Create Assembly Picklists" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="21.09"> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
parents:
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2 <description>Provide a source plate map and an assembly plan, get a robotic picklist spreadsheet for Tecan EVO or Labcyte Echo</description> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
parents:
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3 <macros> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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4 <token name="@VERSION_SUFFIX@">0</token> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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5 <token name="@TOOL_VERSION@">0.1.0</token> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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6 </macros> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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7 <requirements> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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8 <requirement type="package" version="0.18.0">fuzzywuzzy</requirement> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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9 <requirement type="package" version="0.3.4">bandwagon</requirement> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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10 <requirement type="package" version="1.85">biopython</requirement> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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11 <requirement type="package" version="0.2.2">crazydoc</requirement> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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12 <requirement type="package" version="2.0.12">dnacauldron</requirement> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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13 <requirement type="package" version="3.2.16">dnachisel</requirement> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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14 <requirement type="package" version="0.1.11">flametree</requirement> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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15 <requirement type="package" version="0.3.1">plateo</requirement> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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16 <requirement type="package" version="0.1.23">snapgene-reader</requirement> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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17 </requirements> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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18 <command detect_errors="exit_code"><![CDATA[ |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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19 #set genbank_file_paths = ','.join([str(f) for f in $genbank_files]) |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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20 #set file_name_mapping = ",".join(["%s:%s" % (file.file_name, file.name) for file in $genbank_files]) |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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21 |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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22 #set backbone_list = [line.strip() for line in str($backbone_name).strip().split('\n') if line.strip()] |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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23 #set backbone_name = ','.join(backbone_list) |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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24 |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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25 mkdir renamed_parts && |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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26 touch 'picklist.zip' && |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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27 touch 'source_plate.xlsx' && |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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28 |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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29 #for $file in $genbank_files |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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30 cp '$file.file_name' 'renamed_parts/$file.name' && |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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31 #end for |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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32 |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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33 #set renamed_paths = ','.join(['renamed_parts/%s' % file.name for file in $genbank_files]) |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
parents:
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34 |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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35 cp '$source_plate' 'source_plate.xlsx' && |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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36 |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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37 python '$__tool_directory__/CreateAssemblyPicklists_script.py' |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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38 --parts_files '$renamed_paths' |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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39 --picklist '$picklist' |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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40 --source_plate 'source_plate.xlsx' |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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41 --backbone_name '$setting.adv.backbone_name' |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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42 --part_backbone_ratio '$setting.adv.part_backbone_ratio' |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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43 --quantity_unit '$setting.quantity_unit' |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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44 --part_quantity '$setting.part_quantity' |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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45 --buffer_volume '$setting.buffer_volume' |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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46 --total_volume '$setting.total_volume' |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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47 --dispenser '$setting.dispenser' |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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48 --result_zip 'picklist.zip' && |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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49 cp 'picklist.zip' '$output_zip' |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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50 ]]></command> |
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51 <inputs> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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52 <conditional name='setting'> |
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53 <param name="mode" type="select" label="Mode"> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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54 <option value="std" selected="true">Standard</option> |
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55 <option value="demo">DEMO</option> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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56 </param> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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57 <when value="std"> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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58 <param name="quantity_unit" type="select" label="Part quantity unit"> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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59 <option value="fmol" selected="true">femto-mole</option> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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60 <option value="nM">nano-molar</option> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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61 <option value="ng">nano-gram</option> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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62 </param> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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63 <param name="part_quantity" type="float" value="1.3" min="0" label="Part quantity" /> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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64 <param name="buffer_volume" type="float" value="0.3" min="0" label="Buffer volume (µL)" /> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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65 <param name="total_volume" type="float" value="1.0" min="0" label="Total volume (µL)" /> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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66 <param name="dispenser" type="select" label="Dispenser Machine"> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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67 <option value="labcyte_echo" selected="true">Labcyte ECHO</option> |
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68 <option value="tecan_evo">Tecab EVO</option> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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69 </param> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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70 <section name="adv" title="advance" expanded="false"> |
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71 <param name="backbone_name" type="text" area="True" label="Backbone Name(s)" optional='true'/> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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72 <param name="part_backbone_ratio" type="float" value='1.0' min="0" max="1.0" label="Backbone Molar Ratio" optional='true' /> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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73 </section> |
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74 </when> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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75 <when value="demo"> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
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76 <param name="quantity_unit" type="select" label="Part quantity unit"> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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77 <option value="fmol" selected="true">femto-mole</option> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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78 <option value="nM">nano-molar</option> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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79 <option value="ng">nano-gram</option> |
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planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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80 </param> |
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81 <param name="part_quantity" type="float" value="1.3" min="0" label="Part quantity" /> |
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82 <param name="buffer_volume" type="float" value="0.3" min="0" label="Buffer volume (µL)" /> |
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83 <param name="total_volume" type="float" value="1.0" min="0" label="Total volume (µL)" /> |
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84 <param name="dispenser" type="select" label="Dispenser Machine"> |
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85 <option value="labcyte_echo" selected="true">Labcyte ECHO</option> |
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86 <option value="tecan_evo">Tecab EVO</option> |
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87 </param> |
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88 <section name="adv" title="advance" expanded="false"> |
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89 <param name="backbone_name" type="text" area="True" value='HC_Amp_ccdB' label="Backbone Name(s)" optional='true'/> |
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90 <param name="part_backbone_ratio" type="float" value='1.0' min="0" max="1.0" label="Backbone Molar Ratio" optional='true' /> |
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91 </section> |
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92 </when> |
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93 </conditional> |
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94 <param name="genbank_files" type="data_collection" collection_type="list" format="genbank,fasta" label="GenBank File(s)" /> |
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95 <param name="picklist" type="data" format="xlsx,xls,csv" label="Assembly Plan (csv or excel)"/> |
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96 <param name="source_plate" type="data" format="xlsx,xls" label="Source Plate (excel)"/> |
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97 </inputs> |
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98 <outputs> |
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99 <data format="zip" name="output_zip" label="picklist (zip)"/> |
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100 </outputs> |
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101 <tests> |
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102 <!--test type2s_assembly class--> |
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103 <!--python CreateAssemblyPicklists_script.py parts_files "test-data/emma_parts/HC_Amp_ccdB.gb,test-data/emma_parts/conn a-c.gb,test-data/emma_parts/conn b-e.gb,test-data/emma_parts/conn b-l.gb,test-data/emma_parts/conn d-e.gb,test-data/emma_parts/conn d-f.gb,test-data/emma_parts/conn h-ha.gb,test-data/emma_parts/conn h-j.gb,test-data/emma_parts/conn h-k.gb,test-data/emma_parts/conn j-k.gb,test-data/emma_parts/conn l-n.gb,test-data/emma_parts/conn l-r.gb,test-data/emma_parts/conn l-w.gb,test-data/emma_parts/conn l-y.gb,test-data/emma_parts/conn q-r.gb,test-data/emma_parts/conn r-w.gb,test-data/emma_parts/conn t-v.gb,test-data/emma_parts/conn w-x.gb,test-data/emma_parts/conn w-y.gb,test-data/emma_parts/conn w-z.gb,test-data/emma_parts/conn y-z.gb,test-data/emma_parts/p10_tet-aptazyme.gb,test-data/emma_parts/p11_sv40polya.gb,test-data/emma_parts/p14_cmvp.gb,test-data/emma_parts/p14_sv40p.gb,test-data/emma_parts/p15_puror.gb,test-data/emma_parts/p16_bghpolya.gb,test-data/emma_parts/p18_cmvp.gb,test-data/emma_parts/p18_ef1ap.gb,test-data/emma_parts/p19_mneogreen.gb,test-data/emma_parts/p19_mruby2.gb,test-data/emma_parts/p19_mtagbfp2.gb,test-data/emma_parts/p19_tet-on-3g.gb,test-data/emma_parts/p1_5'-itr-pb.gb,test-data/emma_parts/p1_5'ha-haavs1.gb,test-data/emma_parts/p20_ct-minute-nes.gb,test-data/emma_parts/p20_ct-nes.gb,test-data/emma_parts/p20_linker3.gb,test-data/emma_parts/p20_p2a.gb,test-data/emma_parts/p21_dmra.gb,test-data/emma_parts/p21_mkate2.gb,test-data/emma_parts/p21_mneogreen.gb,test-data/emma_parts/p21_mruby2.gb,test-data/emma_parts/p21_mtagbfp2.gb,test-data/emma_parts/p21_puror.gb,test-data/emma_parts/p22_pgkpolya.gb,test-data/emma_parts/p23_insulatorfb.gb,test-data/emma_parts/p24_3'ha_haavs1.gb,test-data/emma_parts/p24_3'pb.gb,test-data/emma_parts/p25_sv40-ori.gb,test-data/emma_parts/p2_insulatorfb.gb,test-data/emma_parts/p3_cagp.gb,test-data/emma_parts/p3_cmvp_tet.gb,test-data/emma_parts/p3_ef1ap.gb,test-data/emma_parts/p3_tre3gp.gb,test-data/emma_parts/p4_kt-l7ae -weiss.gb,test-data/emma_parts/p4_lac-o.gb,test-data/emma_parts/p5_attb-bxb1.gb,test-data/emma_parts/p5_attp-bxb1.gb,test-data/emma_parts/p5_k1-k1.gb,test-data/emma_parts/p5_kt-weiss.gb,test-data/emma_parts/p6_atg_boxc.gb,test-data/emma_parts/p6_kozak-atg.gb,test-data/emma_parts/p6_nt-igkl sequence.gb,test-data/emma_parts/p6_nt-mls.gb,test-data/emma_parts/p6_nt-myristoylation signal.gb,test-data/emma_parts/p6_nt-palm sequence.gb,test-data/emma_parts/p6_nt-sv40_nls.gb,test-data/emma_parts/p7_bxb1.gb,test-data/emma_parts/p7_l7ae-weiss.gb,test-data/emma_parts/p7_l7ae.gb,test-data/emma_parts/p7_laci.gb,test-data/emma_parts/p7_mcherry.gb,test-data/emma_parts/p7_mkate2.gb,test-data/emma_parts/p7_mneogreen.gb,test-data/emma_parts/p7_mruby2.gb,test-data/emma_parts/p7_mtagbfp2.gb,test-data/emma_parts/p8_linker1.gb,test-data/emma_parts/p8_linker2.gb,test-data/emma_parts/p8_p2a.gb,test-data/emma_parts/p8a_ct-kdel.gb,test-data/emma_parts/p8b_ires2.gb,test-data/emma_parts/p9_a-tubulin.gb,test-data/emma_parts/p9_bsdr.gb,test-data/emma_parts/p9_dmrc.gb,test-data/emma_parts/p9_firefly luciferase.gb,test-data/emma_parts/p9_mneogreen.gb,test-data/emma_parts/p9_mruby2.gb,test-data/emma_parts/p9_mtagbfp2.gb,test-data/emma_parts/p9_neor.gb,test-data/emma_parts/p9_puror.gb" backbone_name 'HC_Amp_ccdb' part_backbone_ratio '1.0' quantity_unit 'fmol' part_quantity '1.3' buffer_volume '0.3' total_volume '1.0' dispenser 'labcyte_echo' result_zip 'picklist.zip' picklist 'test-data/example_picklist.xls' source_plate 'test-data/example_echo_plate.xlsx'--> |
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104 <test> |
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105 <param name="genbank_files"> |
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106 <collection type="list"> |
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107 <element name="HC_Amp_ccdB" value="emma_parts/HC_Amp_ccdB.gb" /> |
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108 <element name="conn a-c" value="emma_parts/conn a-c.gb" /> |
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109 <element name="conn b-e" value="emma_parts/conn b-e.gb" /> |
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110 <element name="conn b-l" value="emma_parts/conn b-l.gb" /> |
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111 <element name="conn d-e" value="emma_parts/conn d-e.gb" /> |
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112 <element name="conn d-f" value="emma_parts/conn d-f.gb" /> |
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113 <element name="conn h-ha" value="emma_parts/conn h-ha.gb" /> |
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114 <element name="conn h-j" value="emma_parts/conn h-j.gb" /> |
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115 <element name="conn h-k" value="emma_parts/conn h-k.gb" /> |
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116 <element name="conn j-k" value="emma_parts/conn j-k.gb" /> |
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117 <element name="conn l-n" value="emma_parts/conn l-n.gb" /> |
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118 <element name="conn l-r" value="emma_parts/conn l-r.gb" /> |
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119 <element name="conn l-w" value="emma_parts/conn l-w.gb" /> |
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120 <element name="conn l-y" value="emma_parts/conn l-y.gb" /> |
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121 <element name="conn q-r" value="emma_parts/conn q-r.gb" /> |
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122 <element name="conn r-w" value="emma_parts/conn r-w.gb" /> |
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123 <element name="conn t-v" value="emma_parts/conn t-v.gb" /> |
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124 <element name="conn w-x" value="emma_parts/conn w-x.gb" /> |
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125 <element name="conn w-y" value="emma_parts/conn w-y.gb" /> |
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126 <element name="conn w-z" value="emma_parts/conn w-z.gb" /> |
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127 <element name="conn y-z" value="emma_parts/conn y-z.gb" /> |
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128 <element name="p10_tet-aptazyme" value="emma_parts/p10_tet-aptazyme.gb" /> |
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129 <element name="p11_sv40polya" value="emma_parts/p11_sv40polya.gb" /> |
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130 <element name="p14_cmvp" value="emma_parts/p14_cmvp.gb" /> |
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131 <element name="p14_sv40p" value="emma_parts/p14_sv40p.gb" /> |
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132 <element name="p15_puror" value="emma_parts/p15_puror.gb" /> |
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133 <element name="p16_bghpolya" value="emma_parts/p16_bghpolya.gb" /> |
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134 <element name="p18_cmvp" value="emma_parts/p18_cmvp.gb" /> |
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135 <element name="p18_ef1ap" value="emma_parts/p18_ef1ap.gb" /> |
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136 <element name="p19_mneogreen" value="emma_parts/p19_mneogreen.gb" /> |
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137 <element name="p19_mruby2" value="emma_parts/p19_mruby2.gb" /> |
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138 <element name="p19_mtagbfp2" value="emma_parts/p19_mtagbfp2.gb" /> |
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139 <element name="p19_tet-on-3g" value="emma_parts/p19_tet-on-3g.gb" /> |
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140 <element name="p1_5'-itr-pb" value="emma_parts/p1_5'-itr-pb.gb" /> |
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141 <element name="p1_5'ha-haavs1" value="emma_parts/p1_5'ha-haavs1.gb" /> |
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142 <element name="p20_ct-minute-nes" value="emma_parts/p20_ct-minute-nes.gb" /> |
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143 <element name="p20_ct-nes" value="emma_parts/p20_ct-nes.gb" /> |
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144 <element name="p20_linker3" value="emma_parts/p20_linker3.gb" /> |
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145 <element name="p20_p2a" value="emma_parts/p20_p2a.gb" /> |
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146 <element name="p21_dmra" value="emma_parts/p21_dmra.gb" /> |
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147 <element name="p21_mkate2" value="emma_parts/p21_mkate2.gb" /> |
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148 <element name="p21_mneogreen" value="emma_parts/p21_mneogreen.gb" /> |
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149 <element name="p21_mruby2" value="emma_parts/p21_mruby2.gb" /> |
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150 <element name="p21_mtagbfp2" value="emma_parts/p21_mtagbfp2.gb" /> |
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151 <element name="p21_puror" value="emma_parts/p21_puror.gb" /> |
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152 <element name="p22_pgkpolya" value="emma_parts/p22_pgkpolya.gb" /> |
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153 <element name="p23_insulatorfb" value="emma_parts/p23_insulatorfb.gb" /> |
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154 <element name="p24_3'ha_haavs1" value="emma_parts/p24_3'ha_haavs1.gb" /> |
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155 <element name="p24_3'pb" value="emma_parts/p24_3'pb.gb" /> |
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156 <element name="p25_sv40-ori" value="emma_parts/p25_sv40-ori.gb" /> |
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157 <element name="p2_insulatorfb" value="emma_parts/p2_insulatorfb.gb" /> |
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158 <element name="p3_cagp" value="emma_parts/p3_cagp.gb" /> |
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159 <element name="p3_cmvp_tet" value="emma_parts/p3_cmvp_tet.gb" /> |
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160 <element name="p3_ef1ap" value="emma_parts/p3_ef1ap.gb" /> |
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161 <element name="p3_tre3gp" value="emma_parts/p3_tre3gp.gb" /> |
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162 <element name="p4_kt-l7ae -weiss" value="emma_parts/p4_kt-l7ae -weiss.gb" /> |
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163 <element name="p4_lac-o" value="emma_parts/p4_lac-o.gb" /> |
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164 <element name="p5_attb-bxb1" value="emma_parts/p5_attb-bxb1.gb" /> |
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165 <element name="p5_attp-bxb1" value="emma_parts/p5_attp-bxb1.gb" /> |
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166 <element name="p5_k1-k1" value="emma_parts/p5_k1-k1.gb" /> |
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167 <element name="p5_kt-weiss" value="emma_parts/p5_kt-weiss.gb" /> |
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168 <element name="p6_atg_boxc" value="emma_parts/p6_atg_boxc.gb" /> |
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169 <element name="p6_kozak-atg" value="emma_parts/p6_kozak-atg.gb" /> |
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170 <element name="p6_nt-igkl sequence" value="emma_parts/p6_nt-igkl sequence.gb" /> |
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171 <element name="p6_nt-mls" value="emma_parts/p6_nt-mls.gb" /> |
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172 <element name="p6_nt-myristoylation signal" value="emma_parts/p6_nt-myristoylation signal.gb" /> |
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173 <element name="p6_nt-palm sequence" value="emma_parts/p6_nt-palm sequence.gb" /> |
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174 <element name="p6_nt-sv40_nls" value="emma_parts/p6_nt-sv40_nls.gb" /> |
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175 <element name="p7_bxb1" value="emma_parts/p7_bxb1.gb" /> |
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176 <element name="p7_l7ae-weiss" value="emma_parts/p7_l7ae-weiss.gb" /> |
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177 <element name="p7_l7ae" value="emma_parts/p7_l7ae.gb" /> |
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178 <element name="p7_laci" value="emma_parts/p7_laci.gb" /> |
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179 <element name="p7_mcherry" value="emma_parts/p7_mcherry.gb" /> |
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180 <element name="p7_mkate2" value="emma_parts/p7_mkate2.gb" /> |
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181 <element name="p7_mneogreen" value="emma_parts/p7_mneogreen.gb" /> |
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182 <element name="p7_mruby2" value="emma_parts/p7_mruby2.gb" /> |
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183 <element name="p7_mtagbfp2" value="emma_parts/p7_mtagbfp2.gb" /> |
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184 <element name="p8_linker1" value="emma_parts/p8_linker1.gb" /> |
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185 <element name="p8_linker2" value="emma_parts/p8_linker2.gb" /> |
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186 <element name="p8_p2a" value="emma_parts/p8_p2a.gb" /> |
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187 <element name="p8a_ct-kdel" value="emma_parts/p8a_ct-kdel.gb" /> |
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188 <element name="p8b_ires2" value="emma_parts/p8b_ires2.gb" /> |
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189 <element name="p9_a-tubulin" value="emma_parts/p9_a-tubulin.gb" /> |
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190 <element name="p9_bsdr" value="emma_parts/p9_bsdr.gb" /> |
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191 <element name="p9_dmrc" value="emma_parts/p9_dmrc.gb" /> |
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192 <element name="p9_firefly luciferase" value="emma_parts/p9_firefly luciferase.gb" /> |
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193 <element name="p9_mneogreen" value="emma_parts/p9_mneogreen.gb" /> |
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194 <element name="p9_mruby2" value="emma_parts/p9_mruby2.gb" /> |
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195 <element name="p9_mtagbfp2" value="emma_parts/p9_mtagbfp2.gb" /> |
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196 <element name="p9_neor" value="emma_parts/p9_neor.gb" /> |
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197 <element name="p9_puror" value="emma_parts/p9_puror.gb" /> |
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198 </collection> |
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199 </param> |
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200 <param name="picklist" value="example_picklist.xls" /> |
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201 <param name="source_plate" value="example_echo_plate.xlsx"/> |
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202 <conditional name='setting'> |
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203 <param name="mode" value="std" /> |
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204 <param name="quantity_unit" value="fmol" /> |
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205 <param name="part_quantity" value="1.3" /> |
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206 <param name="buffer_volume" value="0.3" /> |
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207 <param name="total_volume" value="1.0" /> |
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208 <param name="dispenser" value="labcyte_echo" /> |
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209 </conditional> |
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210 <output name="output_zip" ftype='zip'> |
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211 <assert_contents> |
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212 <has_archive_member path=".*" n="5"/> |
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213 </assert_contents> |
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214 </output> |
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215 </test> |
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216 <!--test demo--> |
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217 <test> |
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218 <param name="genbank_files"> |
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219 <collection type="list"> |
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220 <element name="HC_Amp_ccdB" value="emma_parts/HC_Amp_ccdB.gb" /> |
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221 <element name="conn a-c" value="emma_parts/conn a-c.gb" /> |
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222 <element name="conn b-e" value="emma_parts/conn b-e.gb" /> |
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223 <element name="conn b-l" value="emma_parts/conn b-l.gb" /> |
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224 <element name="conn d-e" value="emma_parts/conn d-e.gb" /> |
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225 <element name="conn d-f" value="emma_parts/conn d-f.gb" /> |
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226 <element name="conn h-ha" value="emma_parts/conn h-ha.gb" /> |
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227 <element name="conn h-j" value="emma_parts/conn h-j.gb" /> |
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228 <element name="conn h-k" value="emma_parts/conn h-k.gb" /> |
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229 <element name="conn j-k" value="emma_parts/conn j-k.gb" /> |
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230 <element name="conn l-n" value="emma_parts/conn l-n.gb" /> |
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231 <element name="conn l-r" value="emma_parts/conn l-r.gb" /> |
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232 <element name="conn l-w" value="emma_parts/conn l-w.gb" /> |
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233 <element name="conn l-y" value="emma_parts/conn l-y.gb" /> |
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234 <element name="conn q-r" value="emma_parts/conn q-r.gb" /> |
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235 <element name="conn r-w" value="emma_parts/conn r-w.gb" /> |
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236 <element name="conn t-v" value="emma_parts/conn t-v.gb" /> |
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237 <element name="conn w-x" value="emma_parts/conn w-x.gb" /> |
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238 <element name="conn w-y" value="emma_parts/conn w-y.gb" /> |
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239 <element name="conn w-z" value="emma_parts/conn w-z.gb" /> |
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240 <element name="conn y-z" value="emma_parts/conn y-z.gb" /> |
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241 <element name="p10_tet-aptazyme" value="emma_parts/p10_tet-aptazyme.gb" /> |
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242 <element name="p11_sv40polya" value="emma_parts/p11_sv40polya.gb" /> |
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243 <element name="p14_cmvp" value="emma_parts/p14_cmvp.gb" /> |
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244 <element name="p14_sv40p" value="emma_parts/p14_sv40p.gb" /> |
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245 <element name="p15_puror" value="emma_parts/p15_puror.gb" /> |
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246 <element name="p16_bghpolya" value="emma_parts/p16_bghpolya.gb" /> |
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247 <element name="p18_cmvp" value="emma_parts/p18_cmvp.gb" /> |
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248 <element name="p18_ef1ap" value="emma_parts/p18_ef1ap.gb" /> |
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249 <element name="p19_mneogreen" value="emma_parts/p19_mneogreen.gb" /> |
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250 <element name="p19_mruby2" value="emma_parts/p19_mruby2.gb" /> |
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251 <element name="p19_mtagbfp2" value="emma_parts/p19_mtagbfp2.gb" /> |
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252 <element name="p19_tet-on-3g" value="emma_parts/p19_tet-on-3g.gb" /> |
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253 <element name="p1_5'-itr-pb" value="emma_parts/p1_5'-itr-pb.gb" /> |
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254 <element name="p1_5'ha-haavs1" value="emma_parts/p1_5'ha-haavs1.gb" /> |
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255 <element name="p20_ct-minute-nes" value="emma_parts/p20_ct-minute-nes.gb" /> |
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256 <element name="p20_ct-nes" value="emma_parts/p20_ct-nes.gb" /> |
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257 <element name="p20_linker3" value="emma_parts/p20_linker3.gb" /> |
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258 <element name="p20_p2a" value="emma_parts/p20_p2a.gb" /> |
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259 <element name="p21_dmra" value="emma_parts/p21_dmra.gb" /> |
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260 <element name="p21_mkate2" value="emma_parts/p21_mkate2.gb" /> |
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261 <element name="p21_mneogreen" value="emma_parts/p21_mneogreen.gb" /> |
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262 <element name="p21_mruby2" value="emma_parts/p21_mruby2.gb" /> |
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263 <element name="p21_mtagbfp2" value="emma_parts/p21_mtagbfp2.gb" /> |
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264 <element name="p21_puror" value="emma_parts/p21_puror.gb" /> |
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265 <element name="p22_pgkpolya" value="emma_parts/p22_pgkpolya.gb" /> |
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266 <element name="p23_insulatorfb" value="emma_parts/p23_insulatorfb.gb" /> |
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267 <element name="p24_3'ha_haavs1" value="emma_parts/p24_3'ha_haavs1.gb" /> |
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268 <element name="p24_3'pb" value="emma_parts/p24_3'pb.gb" /> |
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269 <element name="p25_sv40-ori" value="emma_parts/p25_sv40-ori.gb" /> |
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270 <element name="p2_insulatorfb" value="emma_parts/p2_insulatorfb.gb" /> |
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271 <element name="p3_cagp" value="emma_parts/p3_cagp.gb" /> |
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272 <element name="p3_cmvp_tet" value="emma_parts/p3_cmvp_tet.gb" /> |
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273 <element name="p3_ef1ap" value="emma_parts/p3_ef1ap.gb" /> |
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274 <element name="p3_tre3gp" value="emma_parts/p3_tre3gp.gb" /> |
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275 <element name="p4_kt-l7ae -weiss" value="emma_parts/p4_kt-l7ae -weiss.gb" /> |
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276 <element name="p4_lac-o" value="emma_parts/p4_lac-o.gb" /> |
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277 <element name="p5_attb-bxb1" value="emma_parts/p5_attb-bxb1.gb" /> |
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278 <element name="p5_attp-bxb1" value="emma_parts/p5_attp-bxb1.gb" /> |
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279 <element name="p5_k1-k1" value="emma_parts/p5_k1-k1.gb" /> |
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280 <element name="p5_kt-weiss" value="emma_parts/p5_kt-weiss.gb" /> |
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281 <element name="p6_atg_boxc" value="emma_parts/p6_atg_boxc.gb" /> |
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282 <element name="p6_kozak-atg" value="emma_parts/p6_kozak-atg.gb" /> |
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283 <element name="p6_nt-igkl sequence" value="emma_parts/p6_nt-igkl sequence.gb" /> |
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284 <element name="p6_nt-mls" value="emma_parts/p6_nt-mls.gb" /> |
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285 <element name="p6_nt-myristoylation signal" value="emma_parts/p6_nt-myristoylation signal.gb" /> |
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286 <element name="p6_nt-palm sequence" value="emma_parts/p6_nt-palm sequence.gb" /> |
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287 <element name="p6_nt-sv40_nls" value="emma_parts/p6_nt-sv40_nls.gb" /> |
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288 <element name="p7_bxb1" value="emma_parts/p7_bxb1.gb" /> |
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289 <element name="p7_l7ae-weiss" value="emma_parts/p7_l7ae-weiss.gb" /> |
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290 <element name="p7_l7ae" value="emma_parts/p7_l7ae.gb" /> |
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291 <element name="p7_laci" value="emma_parts/p7_laci.gb" /> |
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292 <element name="p7_mcherry" value="emma_parts/p7_mcherry.gb" /> |
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293 <element name="p7_mkate2" value="emma_parts/p7_mkate2.gb" /> |
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294 <element name="p7_mneogreen" value="emma_parts/p7_mneogreen.gb" /> |
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295 <element name="p7_mruby2" value="emma_parts/p7_mruby2.gb" /> |
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296 <element name="p7_mtagbfp2" value="emma_parts/p7_mtagbfp2.gb" /> |
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297 <element name="p8_linker1" value="emma_parts/p8_linker1.gb" /> |
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298 <element name="p8_linker2" value="emma_parts/p8_linker2.gb" /> |
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299 <element name="p8_p2a" value="emma_parts/p8_p2a.gb" /> |
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300 <element name="p8a_ct-kdel" value="emma_parts/p8a_ct-kdel.gb" /> |
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301 <element name="p8b_ires2" value="emma_parts/p8b_ires2.gb" /> |
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302 <element name="p9_a-tubulin" value="emma_parts/p9_a-tubulin.gb" /> |
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303 <element name="p9_bsdr" value="emma_parts/p9_bsdr.gb" /> |
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304 <element name="p9_dmrc" value="emma_parts/p9_dmrc.gb" /> |
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305 <element name="p9_firefly luciferase" value="emma_parts/p9_firefly luciferase.gb" /> |
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306 <element name="p9_mneogreen" value="emma_parts/p9_mneogreen.gb" /> |
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307 <element name="p9_mruby2" value="emma_parts/p9_mruby2.gb" /> |
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308 <element name="p9_mtagbfp2" value="emma_parts/p9_mtagbfp2.gb" /> |
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309 <element name="p9_neor" value="emma_parts/p9_neor.gb" /> |
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310 <element name="p9_puror" value="emma_parts/p9_puror.gb" /> |
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311 </collection> |
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312 </param> |
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313 <param name="picklist" value="example_picklist.xls" /> |
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314 <param name="source_plate" value="example_echo_plate.xlsx"/> |
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315 <conditional name='setting'> |
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316 <param name="mode" value="demo" /> |
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317 </conditional> |
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318 <output name="output_zip" ftype='zip'> |
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319 <assert_contents> |
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320 <has_archive_member path=".*" n="5"/> |
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321 </assert_contents> |
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322 </output> |
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323 </test> |
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324 </tests> |
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325 |
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326 <help><![CDATA[ |
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327 create_assembly_picklists |
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328 ========================= |
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329 |
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330 Provide a source plate map and an assembly plan, get a robotic picklist spreadsheet for Tecan EVO or Labcyte Echo. |
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331 `Read more... <https://github.com/Edinburgh-Genome-Foundry/Plateo/blob/master/README.rst>`_ |
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332 |
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333 **Advanced parameters:** |
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334 ------------------------ |
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335 |
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336 - **Backbone Name(s):** It can be one backbone or more than one (one name per line). |
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337 - **Backbone Molar Ratio:** Between 0.0 and 1.0. This multiplies the backbone concentration with the part:backbone molar ratio. |
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338 For example, a part:backbone = 2:1 will multiply the backbone concentration by 2, so half as much of it will be added. |
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339 **Note:** If the part quantity unit is ng, this calculation is not performed. |
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340 |
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341 **Mode:** |
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342 --------- |
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343 |
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344 This option allows you to choose how the tool runs: |
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345 |
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346 - **Standard**: Runs the tool as a standalone application. |
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347 - **DEMO**: Runs the tool with demo data. |
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348 |
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349 Demo data: |
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350 |
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351 - **GenBank File(s):** `Download GenBank files <https://files.osf.io/v1/resources/x2sj3/providers/osfstorage/689496483e21964670f62efc/?zip=>`_ |
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352 |
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353 **Note:** Make GenBank files as a collection list. |
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354 `How to make collection <https://training.galaxyproject.org/training-material/topics/galaxy-interface/tutorials/collections/tutorial.html>`_ |
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355 |
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356 - **Assembly Plan (CSV or Excel):** `Download Assembly Plan <https://osf.io/download/6894965a96fb8cc5376b0c92/>`_ |
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357 |
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358 - **Source Plate (Excel):** `Download Source Plate <https://osf.io/download/6894965b7d01c1cf281b52d3/>`_ |
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359 ]]></help> |
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360 |
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361 |
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362 <citations> |
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363 <citation type="bibtex"> |
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364 @unpublished{create_assembly_picklists |
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tduigou
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365 author = {Ramiz Khaled}, |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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366 title = {{create_assembly_picklists}}, |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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367 url = {https://github.com/Edinburgh-Genome-Foundry/CUBA/blob/master/backend/app/views/create_assembly_picklists/CreateAssemblyPicklistsView.py}, |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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368 } |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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369 </citation> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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370 </citations> |
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0b38f1504205
planemo upload for repository https://github.com/Edinburgh-Genome-Foundry/Plateo commit cc895a281863630e391c310322fbddfd31ac1f8f-dirty
tduigou
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371 </tool> |
