LOCUS       .                       2532 bp    DNA              UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     terminator      392..419
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(431..1018)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      1040..1069
                     /note="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     misc_feature    1143..1148
                     /note="BsmBI"
     source          5..1149
                     /source="Exported"
     misc_feature    1154..1164
                     /note="KozaK"
     CDS             1162..1164
                     /codon_start=1
                     /note="ATG"
                     /translation="M"
     CDS             1165..1872
                     /codon_start=1
                     /note="mRuby2"
                     /product="monomeric red fluorescent protein derived from
                     mRuby, with improved photophysical properties (Lam et al.,
                     2012)"
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     source          1154..1872
                     /source="Exported"
     misc_feature    complement(1878..1883)
                     /note="BsmBI"
     terminator      1950..1981
                     /note="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        1982..2084
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene"
     source          1877..2532
                     /source="Exported"
ORIGIN
        1 ctcaggcgca atcacgaatg aataacggtt tggttggtgc gagtgatttt gatgacgagc
       61 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac
      121 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
      181 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
      241 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
      301 aatatggtat tgataatcct gatatgaata aattgcagtt tcacttgatg ctcgatgagt
      361 ttttctaatg agggcccaaa tgtaatcacc tggctcacct tcgggtgggc ctttctgcgt
      421 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgatgctcaa
      481 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      541 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      601 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      661 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      721 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      781 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      841 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      901 agccagttac ctcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      961 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1021 agatcctttg attttctacc gaagaaaggc ccacccgtga aggtgagcca gtgagttgat
     1081 tgcagtccag ttacgctgga gtctgaggct cgtcctgaat gatatcaagc ttgaattcgt
     1141 tacgtctcgg tgcccgccac catggtgtct aagggcgaag agctgatcaa ggaaaatatg
     1201 cgtatgaagg tggtcatgga aggttcggtc aacggccacc aattcaaatg cacaggtgaa
     1261 ggagaaggca atccgtacat gggaactcaa accatgagga tcaaagtcat cgagggagga
     1321 cccctgccat ttgcctttga cattcttgcc acgtcgttca tgtatggcag ccgtactttt
     1381 atcaagtacc cgaaaggcat tcctgatttc tttaaacagt cctttcctga gggttttact
     1441 tgggaaagag ttacgagata cgaagatggt ggagtcgtca ccgtcatgca ggacaccagc
     1501 cttgaggatg gctgtctcgt ttaccacgtc caagtcagag gggtaaactt tccctccaat
     1561 ggtcccgtga tgcagaagaa gaccaagggt tgggagccta atacagagat gatgtatcca
     1621 gcagatggtg gtctgagggg atacactcat atggcactga aagttgatgg tggtggccat
     1681 ctgtcttgct ctttcgtaac aacttacagg tcaaaaaaga ccgtcgggaa catcaagatg
     1741 cccggtatcc atgccgttga tcaccgcctg gaaaggttag aggaaagtga caatgaaatg
     1801 ttcgtagtac aacgcgaaca cgcagttgcc aagttcgccg ggcttggtgg tgggatggac
     1861 gagctgtaca agagcgcgag acgaagacga attctctaga tatcgctcaa tactgaccat
     1921 ttaaatcata cctgacctcc atagcagaaa gtcaaaagcc tccgaccgga ggcttttgac
     1981 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     2041 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatgagc catattcaac
     2101 gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     2161 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga
     2221 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     2281 cagatgagat ggtcaggcta aactggctga cggaatttat gcctcttccg accatcaagc
     2341 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatccca gggaaaacag
     2401 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     2461 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac ggcgatcgcg
     2521 tatttcgtct cg
//