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comparison test-data/HC_Amp_ccdB.gb @ 0:7ff266aecf01 draft default tip

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planemo upload for repository https://github.com/brsynth/galaxytools/tree/main/tools commit 3401816c949b538bd9c67e61cbe92badff6a4007-dirty
author tduigou
date Wed, 11 Jun 2025 09:42:24 +0000
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-1:000000000000 0:7ff266aecf01
1 LOCUS Exported 2721 bp ds-DNA circular SYN 14-SEP-2017
2 DEFINITION synthetic circular DNA.
3 ACCESSION .
4 VERSION .
5 KEYWORDS HC_Amp_ccdB(1-25).gb
6 SOURCE synthetic DNA construct
7 ORGANISM synthetic DNA construct
8 REFERENCE 1 (bases 1 to 2721)
9 AUTHORS Trial User
10 TITLE Direct Submission
11 JOURNAL Exported Sep 14, 2017 from SnapGene Viewer 4.0.2
12 http://www.snapgene.com
13 FEATURES Location/Qualifiers
14 source 1..2721
15 /organism="synthetic DNA construct"
16 /mol_type="other DNA"
17 misc_feature complement(73..78)
18 /label=BsmBI
19 misc_feature 129..462
20 /label=*ccdB promoter*
21 exon 463..768
22 /label=ccdB
23 /note="ccdB"
24 terminator 809..880
25 /note="rrnB T1 terminator
26 transcription terminator T1 from the E. coli rrnB gene"
27 terminator 896..923
28 /note="T7Te terminator
29 phage T7 early transcription terminator"
30 misc_feature 930..942
31 /label=BioBrick suffix
32 /note="universal suffix for all parts"
33 misc_feature 943..948
34 /label=BsmBI
35 terminator 1027..1056
36 /note="T3Te terminator
37 phage T3 early transcription terminator"
38 rep_origin 1078..1665
39 /direction=RIGHT
40 /label=ori
41 /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
42 replication"
43 terminator 1677..1704
44 /note="T7Te terminator
45 phage T7 early transcription terminator"
46 CDS complement(1728..2588)
47 /codon_start=1
48 /gene="bla"
49 /product="beta-lactamase"
50 /note="AmpR
51 confers resistance to ampicillin, carbenicillin, and
52 related antibiotics"
53 /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
54 ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
55 PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
56 EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
57 LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
58 LIKHW"
59 promoter complement(2589..2691)
60 /note="cat promoter
61 promoter of the E. coli cat gene"
62 ORIGIN
63 1 ctttctgcta tggaggtcag gtatgattta aatggtcagt attgagcgat atctagagaa
64 61 ttcgtcatag gagagacgca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
65 121 aatgcaggga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
66 181 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
67 241 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
68 301 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
69 361 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
70 421 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
71 481 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
72 541 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
73 601 ccccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
74 661 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
75 721 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataagc tgatagtgct
76 781 agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
77 841 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
78 901 accttcgggt gggcctttct gcgtttatat actagtagcg gccgtctcaa cgataacgaa
79 961 ttcaagcttg atatcattca ggacgagcct cagactccag cgtaactgga ctgcaatcaa
80 1021 ctcactggct caccttcacg ggtgggcctt tcttcggtag aaaatcaaag gatcttcttg
81 1081 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
82 1141 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaggtaac tggcttcagc
83 1201 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
84 1261 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
85 1321 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
86 1381 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
87 1441 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
88 1501 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
89 1561 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
90 1621 catcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgca
91 1681 gaaaggccca cccgaaggtg agccaggtga ttacatttgg gccctcatta ccaatgctta
92 1741 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
93 1801 cccgtcgtgt agataactac gatgcgggag ggcttaccat ctggccccag tgctgcaatg
94 1861 ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca gccagccggg
95 1921 agggccgagc gcagaagtga tcctgcaact ttatccgcct ccatccagtc tattaattgt
96 1981 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
97 2041 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
98 2101 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
99 2161 ggtcctccga tcgttgccag aagtaagttg gccgcagtgt tatcactcat ggttatggca
100 2221 gcactgcata attctcttac tgtcatgcca tccgtgagat gcttttctgt gactggtgag
101 2281 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
102 2341 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
103 2401 cgttcttcgg ggcgtaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
104 2461 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
105 2521 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
106 2581 atactcattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
107 2641 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aagtcaaaag
108 2701 cctccggtcg gaggcttttg a
109 //