5' UTR including B0032 RBS and BB scars (long and short)
Designs for round 1 if iGEM 2021 calibration experimentation
BioBricks scar including ag (not before CDS)
BioBricks scar (short one, w/o ag, before CDS)
Cyan FP; codon optimized using IDT tool <https://www.idtdna.com/CodonOpt> ECFP
Final products desired for actual fabrication
consensus without runs of As and Ts -59 to -36
Swapped R0040 for TwistNeg as the negative control, due to Twist minimum synthesis length
Cyan FP (free); codon optimized using IDT tool <https://www.idtdna.com/CodonOpt> meffCFP
Blue FP (free); codon optimized using IDT tool <https://www.idtdna.com/CodonOpt> mmilCFP
Cyan FP; codon optimized using IDT tool <https://www.idtdna.com/CodonOpt> mCerulean3