# HG changeset patch # User thomaswollmann # Date 1547670850 18000 # Node ID 3cbb8ae57f5bcc5a39ec07bf0ea56d20657e9931 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/coordinates_of_roi/ commit d93e1dd276027cfc3fb518236110395a23d96f66 diff -r 000000000000 -r 3cbb8ae57f5b coordinates_of_roi.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/coordinates_of_roi.py Wed Jan 16 15:34:10 2019 -0500 @@ -0,0 +1,40 @@ +import argparse +import pandas as pd +import skimage.io +import skimage.color +import warnings + +def get_pixel_values(im, pixel_table, white_obj, threshold, offset=[0,0]): + data = skimage.io.imread(im) + if len(data.shape) == 3 and data.shape[-1] > 1: + data = skimage.color.rgb2grey(data) + x = [] + y = [] + for i in range(data.shape[0]): + for j in range(data.shape[1]): + if white_obj == False: + if data[i,j] <= threshold: + x.append(j + offset[0]) + y.append(i + offset[1]) + elif data[i,j] >= threshold: + x.append(j + offset[0]) + y.append(i + offset[1]) + + df = pd.DataFrame() + df['x'] = x + df['y'] = y + df.to_csv(pixel_table, sep="\t", index = False) + +if __name__=="__main__": + parser = argparse.ArgumentParser(description = "Create a csv table with Coordinates of the ROI") + parser.add_argument("im", help = "Paste path to out.png (output created by transformation)") + parser.add_argument("pixel_table", help = "Paste path to file in which list with all pixles > threshold should be saved") + parser.add_argument('offset_x', type=int, help='offset in x direction (width)', default=0) + parser.add_argument('offset_y', type=int, help='offset in y direction (height)', default=0) + parser.add_argument("--white_obj", dest = "white_obj", default=False, help = "If set objects in image are white otherwise black", action = "store_true") + parser.add_argument("--threshold", dest = "threshold", default = 0.5, help = "Enter desired threshold value", type = float) + + args = parser.parse_args() + # with warnings.catch_warnings(): + # warnings.simplefilter("ignore") + get_pixel_values(args.im, args.pixel_table, args.white_obj, args.threshold, [args.offset_x, args.offset_y]) diff -r 000000000000 -r 3cbb8ae57f5b coordinates_of_roi.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/coordinates_of_roi.xml Wed Jan 16 15:34:10 2019 -0500 @@ -0,0 +1,48 @@ + + + Coordinates of ROI + + scikit-image + pandas + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + This tool creates a table containing the pixelcoordinates of the Region of Interest (ROI). + + + 10.1016/j.jbiotec.2017.07.019 + + diff -r 000000000000 -r 3cbb8ae57f5b test-data/table.csv --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/table.csv Wed Jan 16 15:34:10 2019 -0500 @@ -0,0 +1,7 @@ +x y +4 5 +5 5 +6 5 +7 5 +8 5 +9 5 diff -r 000000000000 -r 3cbb8ae57f5b test-data/table2.csv --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/table2.csv Wed Jan 16 15:34:10 2019 -0500 @@ -0,0 +1,7 @@ +x y +5 7 +6 7 +7 7 +8 7 +9 7 +10 7 diff -r 000000000000 -r 3cbb8ae57f5b test-data/test.png Binary file test-data/test.png has changed