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comparison galaxy-conf/FastqIlluminaToSanger.xml @ 24:a77e126ae856 draft

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Reupload since last upload did not load correctly
author timpalpant
date Tue, 19 Jun 2012 22:15:09 -0400
parents 9d56b5b85740
children b43c420a6135
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23:01d5d20eaadd 24:a77e126ae856
1 <tool id="FastqIlluminaToSanger" name="Convert Fastq qualities" version="1.0.0">
2 <description>from Illumina to Sanger</description>
3 <command interpreter="sh">galaxyToolRunner.sh converters.FastqIlluminaToSanger -i $input -o $output</command>
4 <inputs>
5 <param name="input" type="data" format="fastqillumina" label="FASTQ, Illumina qualities" />
6 </inputs>
7 <outputs>
8 <data name="output" format="fastqsanger" metadata_source="input" />
9 </outputs>
10 <tests>
11 <test>
12 <param name="input" value="test.fastqillumina" ftype="fastqillumina"/>
13 <output name="output" file="test.fastqsanger"/>
14 </test>
15 </tests>
16 <help>
17
18 This tool will convert a FASTQ file with ASCII quality scores encoded in Illumina 1.3-1.7 format (Phred+64) to Sanger format (Phred+33) for use with Bowtie and other Galaxy tools. Illumina CASAVA >= 1.8 already produces FASTQ files in Sanger format, so this tool should not be used on new Illumina sequencing data. This tool is a simpler, faster version of the FASTQ Groomer that does little error checking but performs much faster. If you are unsure what format your file is in, or need to do other conversions, use the FASTQ Groomer instead.
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20 For more information, read about FASTQ formats_ (right-click to open in new window).
21
22 .. _formats: http://en.wikipedia.org/wiki/FASTQ_format
23
24 .. class:: warningmark
25
26 This tool requires fastqillumina formatted data. If you have fastq data that was not correctly autodetected, change the metadata by clicking on the pencil icon for the dataset.
27
28 </help>
29 </tool>