Mercurial > repos > timpalpant > java_genomics_toolkit
diff galaxy-conf/BaseAlignCounts.xml @ 13:eb53be9a09f4 draft
Uploaded
author | timpalpant |
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date | Sat, 19 May 2012 10:36:45 -0400 |
parents | 81d5b81fb3c2 |
children | 3e477c7e0e73 |
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--- a/galaxy-conf/BaseAlignCounts.xml Wed Apr 25 16:53:48 2012 -0400 +++ b/galaxy-conf/BaseAlignCounts.xml Sat May 19 10:36:45 2012 -0400 @@ -3,12 +3,13 @@ <command interpreter="sh">galaxyToolRunner.sh ngs.BaseAlignCounts -i $input -a ${chromInfo} -x $X -o $output</command> <inputs> <param name="input" type="data" format="sam,bam,bed,bedgraph" label="Sequencing reads" /> - <param name="X" type="integer" value="-1" label="In silico extension (-1 for read length)" /> + <param name="X" type="integer" value="-1" label="In silico extension (-1 for fragment length)" /> </inputs> <outputs> <data name="output" format="wig" /> </outputs> - + <tests> + </tests> <help> This tool produces a new Wig file with the number of reads/intervals overlapping each base pair. Reads can be artificially extended to match known fragment lengths. If you wish to count the number of reads starting at each base pair, set the read extension to 1. If you wish to count the number of intervals overlapping each base pair, set the extension to -1. @@ -19,6 +20,10 @@ This tool requires sequencing reads in SAM, BAM, Bed, or BedGraph format. If you are artificially extending reads, ensure that the strand is set correctly in SAM, BAM, and Bed files. +.. class:: warningmark + +Paired-end reads are considered to be the entire fragment (the distance from the 5' end of mate 1 to the 5' end of mate 2) if the extension is set to -1. + .. class:: infomark If you would like to convert valued interval data (e.g. BedGraph files from microarrays) to Wig format, use the Converters -> Interval to Wig converter.