view galaxy-conf/ZScore.xml @ 22:727fbba02ef7 draft

Fix a bug in GeneTrackToWig where +/- strands were not merged correctly. Add option to vary the chunk size of tools that process chromosomes in chunks.
author timpalpant
date Tue, 19 Jun 2012 22:09:23 -0400
parents 9d56b5b85740
children
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<tool id="WigZScore" name="Z-Score" version="1.2.0">
  <description>a (Big)Wig file</description>
  <command interpreter="sh">galaxyToolRunner.sh wigmath.ZScore -i $input $chr -o $output</command>
  <inputs>
      <param format="bigwig,wig" name="input" type="data" label="Z-score the data in" />
      <param name="chr" type="boolean" checked="false" truevalue="--bychr" falsevalue="" label="Z-score chromosomes individually" />
  </inputs>
  <outputs>
      <data format="wig" name="output" metadata_source="input" />
  </outputs>
  <tests>
	  <test>
	    <param name="input" value="wigmath1.wig"/>
	    <output name="output" file="zscorer1.wig"/>
	  </test>
	  <test>
	    <param name="input" value="wigmath2.wig"/>
	    <output name="output" file="zscorer2.wig"/>
	  </test>
	  <test>
	    <param name="input" value="wigmath3.wig"/>
	    <output name="output" file="zscorer3.wig"/>
	  </test>
	  <test>
	    <param name="input" value="wigmath1.bw"/>
	    <output name="output" file="zscorer4.wig"/>
	  </test>
	  <test>
	    <param name="input" value="wigmath2.bw"/>
	    <output name="output" file="zscorer5.wig"/>
	  </test>
	  <test>
	    <param name="input" value="wigmath3.bw"/>
	    <output name="output" file="zscorer6.wig"/>
	  </test>
  </tests>
  
  <help>

This tool will compute normal scores (Z-scores) for each of the values in a Wig file. For each base pair, the Z-scored value is equal to the deviance from the mean divided by the standard deviation (i.e. the number of standard deviations a value is away from the mean). The output file should have mean 0 and standard deviation 1.

.. class:: infomark

This tool is equivalent to using the **Mean Shift** tool to shift a Wig file to mean 0, then using the **Scale** tool to scale by 1/(standard deviation).

.. class:: infomark

**TIP:** If your dataset does not appear in the pulldown menu, it means that it is not in Wig or BigWig format. Use "edit attributes" to set the correct format if it was not detected correctly.

  </help>
</tool>