Mercurial > repos > triasteran > ribogalaxy_umi_processing

diff UMI_riboseq_processing/UMI.py @ 2:6958515efa76 draft

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author triasteran
date Mon, 20 Jun 2022 07:27:23 +0000
parents 5d0d5933d370
children d27375bc4a1c
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line diff
--- a/UMI_riboseq_processing/UMI.py	Sun Jun 19 12:44:06 2022 +0000
+++ b/UMI_riboseq_processing/UMI.py	Mon Jun 20 07:27:23 2022 +0000
@@ -20,7 +20,7 @@
             sep = lines[2]
             qual = lines[3]
             trimmed_seq = seq[2:-5]+"\n" # fooprint + barcode
-            UMI = seq[0:2]+seq[-5:] #7nt in total; 5'NN and last 3'NNNNN  
+            UMI = seq[0:2]+seq[-5:].strip('\n') #7nt in total; 5'NN and last 3'NNNNN  
             split_header = header.split(" ")
             new_header = split_header[0]+"_"+UMI+" "+split_header[1]
             if qual[-1:] == "\n":