Mercurial > repos > triasteran > trips_create_new_organism
diff trips_create_new_organism/create_annotation_sqlite_.xml @ 0:c5a566609a25 draft
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author | triasteran |
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date | Fri, 25 Feb 2022 11:24:50 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/trips_create_new_organism/create_annotation_sqlite_.xml Fri Feb 25 11:24:50 2022 +0000 @@ -0,0 +1,71 @@ +<tool id="create_annotation_sqlite" name="Trips-viz:" version="0.1.0"> + <description>create new organism</description> + <command interpreter="python">create_annotation_sqlite.py $annotation $fasta $pseudo_utr_len $transcript $gene $output</command> + <inputs> + <param format="gtf,gff" name="annotation" type="data" label="GTF/GFF3 File"/> + <param format="fasta" name="fasta" type="data" label="Transcriptome FASTA file"/> + <param name="pseudo_utr_len" type="text" label="Pseudo UTR length"/> + <param name="transcript" type="text" label="Example transcript"/> + <param name="gene" type="text" label="Example gene"/> + </inputs> + + <outputs> + <data format="sqlite" name="output" /> + </outputs> + + <tests> + <test> + <param name="input" value="fa_gc_content_input.fa"/> + <output name="output" file="saccharomyces_cerevisiae.sqlite"/> + </test> + </tests> + + <help> + +**GTF/GFF3 File** + +GFF lines have nine required fields that must be tab-separated. +The GFF3 format addresses the most common extensions to GFF, while preserving backward compatibility with previous formats. + +Both transcript ids and gene names should be listed in the file. + +----- + +**Transcriptome FASTA file** + +A FASTA file with an entry for every transcript. The headers should be the transcript id's as they appear in the GTF/GFF3 file. + +----- + +**Psuedo UTR length** +An integer representing the length (in nucleotides) to be added to the 5' end and 3' end of every transcript with an annotated +CDS. Useful for when an organism does not have any annotated UTR's, if it does use 0. If not 0, the extra nucleotides should +already be present in the FASTA file. + +----- + +**Example transcript** + +An example of a transcript id that appears in the FASTA/GTF/GFF3 file, e.g ENST00000123456 + +----- + +**Example Gene** + +An example of a gene name as it appears in the GTF/GFF3 file, e.g BRCA1 + +----- + +**Output** + +The output of the script can be downloaded and uploaded to Trips-viz_. by signing in and going to the uploads page, then selecting +"Upload new transcriptome". When uploaded the new organism will appear on the home page of Trips-viz, or under the transcriptomes +page if the organism name used is already present on Trips-viz. + + + +.. _Trips-viz: http://trips.ucc.ie + + +</help> +</tool>