Mercurial > repos > trinity_ctat > ctat_abundance_estimation_to_matrix
changeset 0:5eca0c75b178 draft default tip
Upload ctat tools.
| author | trinity_ctat |
|---|---|
| date | Tue, 17 Jul 2018 11:47:32 -0400 |
| parents | |
| children | |
| files | ctat_abundance_estimation_to_matrix.py ctat_abundance_estimation_to_matrix.xml ctat_trinity_tool_wrapper.py test-data/Sp_ds.RSEM.genes.results test-data/Sp_hs.RSEM.genes.results |
| diffstat | 5 files changed, 970 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/ctat_abundance_estimation_to_matrix.py Tue Jul 17 11:47:32 2018 -0400 @@ -0,0 +1,40 @@ +#!/usr/bin/env python + +import sys, os, string, subprocess + +#aliasing the filenames using the labels + + +def run_command(command): + print "Running command: " + command + + err_capture_file = open("my.stderr", 'w') # writing stderr to a file + cmd_run = subprocess.Popen(args=command, shell=True, stderr=err_capture_file, stdout=sys.stdout) + err = cmd_run.wait() # get exit code from command execution + err_capture_file.close() + + if err: + # report the error messages we captured, and exit non-zero + sys.stderr.write("Error, cmd: " + command + " died with ret: " + `err`) + for line in open(err_capture_file): + sys.stderr.write(line) + sys.exit(err) + return + +label_list = [] # symlink files to the labels +for i in range(1, len(sys.argv), 2): + filename=sys.argv[i] + label= sys.argv[i+1] + cmd= "ln -sf " + filename + " " + label + label_list.append(label) + run_command(cmd) + + +# run the abundance estimation script + +cmd = os.path.dirname(sys.argv[0]) + "/ctat_trinity_tool_wrapper.py " + " util/abundance_estimates_to_matrix.pl --gene_trans_map none --est_method RSEM --cross_sample_norm TMM " + " ".join(label_list) + +run_command(cmd) + +sys.exit(0) +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/ctat_abundance_estimation_to_matrix.xml Tue Jul 17 11:47:32 2018 -0400 @@ -0,0 +1,101 @@ +<tool id="ctat_abundance_estimation_to_matrix" name="ctat_abundance_estimation_to_matrix" version="1.0.0" profile="17.05"> + + <description>Join RSEM estimates from multiple samples into a single matrix</description> + <requirements> + <requirement type="package" version="2.7">python</requirement> + <requirement type="package">subprocess32</requirement> + <requirement type="package">bzip2</requirement> + <requirement type="package" version="1.3.0">rsem</requirement> + <requirement type="package" version="3">bioconductor-edger</requirement> + <requirement type="package" version="2">bioconductor-qvalue</requirement> + <requirement type="package" version="2.6.6">trinity</requirement> + </requirements> + <command detect_errors="exit_code"> + <![CDATA[ + python $__tool_directory__/ctat_abundance_estimation_to_matrix.py +#for $q in $RSEM_samples +${q.file} "${q.column_label}" +#end for + ]]> + + </command> + <inputs> + + <repeat name="RSEM_samples" title="RSEM abundance estimates for samples"> + <param name="file" label="Add file" type="data" format="txt"/> + <param name="column_label" label="column label" type="text" /> + </repeat> + + </inputs> + <outputs> + <data format="tabular" name="counts_matrix" label="${tool.name} on ${on_string}: Counts Matrix" from_work_dir="RSEM.isoform.counts.matrix"/> + <data format="tabular" name="tmm_expr_matrix" label="${tool.name} on ${on_string}: TMM EXPR Matrix" from_work_dir="RSEM.isoform.TMM.EXPR.matrix"/> +</outputs> + <tests> +<test> + <repeat name="RSEM_samples"> + <param name="file" value="Sp_ds.RSEM.genes.results" /> + <param name="column_label" value="Sp_ds" /> + </repeat> + <repeat name="RSEM_samples"> + <param name="file" value="Sp_hs.RSEM.genes.results" /> + <param name="column_label" value="Sp_hs" /> + </repeat> + + <output name="counts_matrix" > + <assert_contents> + <has_line_matching expression=".+" /> + <has_line line="	Sp_ds	Sp_hs" /> + <has_n_columns n="3" /> + <has_line_matching expression="TRINITY_DN.+" /> + </assert_contents> + </output> + <output name="tmm_expr_matrix" > + <assert_contents> + <has_line_matching expression=".+" /> + <has_line line="	Sp_ds	Sp_hs" /> + <has_n_columns n="3" /> + <has_line_matching expression="TRINITY_DN.+" /> + </assert_contents> + </output> + </test> + <!-- The following test has not been tested to see if it works. +<test> + <repeat name="RSEM_samples"> + <param name="file" value="Sp_ds.RSEM.isoforms.results" /> + <param name="column_label" value="Sp_ds" /> + </repeat> + <repeat name="RSEM_samples"> + <param name="file" value="Sp_hs.RSEM.isoforms.results" /> + <param name="column_label" value="Sp_hs" /> + </repeat> + + <output name="counts_matrix" > + <assert_contents> + <has_line_matching expression=".+" /> + </assert_contents> + </output> + <output name="tmm_expr_matrix" > + <assert_contents> + <has_line_matching expression=".+" /> + </assert_contents> + </output> + </test> + --> + </tests> + <help> +.. class:: infomark + +This step will join the RSEM-computed gene or isoform fragment counts into a matrix file, which will be used to run edgeR and identify differentially expressed transcripts in next few steps. Execution of this will generate a counts matrix file with a name 'abundance_estimation_to_matrix: counts_matrix'. + +If you are following the Trinity RNA-seq protocol please go here_ for a galaxy tool walk through or the Nature Protocols paper_. + +.. _here: https://github.com/trinityrnaseq/GalaxyTrinityProtocol/wiki +.. _paper: http://www.nature.com/nprot/journal/v8/n8/full/nprot.2013.084.html + </help> + + <citations> + <citation type="doi">10.1038/nbt.1883</citation> + </citations> + +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/ctat_trinity_tool_wrapper.py Tue Jul 17 11:47:32 2018 -0400 @@ -0,0 +1,123 @@ +#!/usr/bin/env python + + +# borrowed from: http://wiki.g2.bx.psu.edu/Future/Job%20Failure%20When%20stderr and modified for use with Trinity tools. + +""" +Wrapper that execute a program and its arguments but reports standard error +messages only if the program exit status was not 0 +Example: ./stderr_wrapper.py myprog arg1 -f arg2 +""" + +import sys, subprocess, os +print sys.version + +assert sys.version_info[:2] >= ( 2, 4 ) + +TRINITY_BASE_DIR = "" +if os.environ.has_key('TRINITY_HOME'): + TRINITY_BASE_DIR = os.environ['TRINITY_HOME']; +elif hasattr(os, 'symlink'): # symlink was implemented to always return false when it was not implemented in earlier versions. + # 2017-09-26 + # Cicada Dennis added looking for the location of the Trinity program using the Unix "which" utility. + # I tried using "command -v Trinity" but for some reason, I was getting a OS permission error with that. + # I just found distutils.spawn.find_executable() which might work, but already implemented the below. + try: + pipe1 = subprocess.Popen(["which", "Trinity"], stdout=subprocess.PIPE) + except: + msg = "You must set the environmental variable TRINITY_HOME to the base installation directory of Trinity before running {:s}.\n".format(sys.argv[0]) + sys.stderr.write(msg) + # t, v, tb = sys.exc_info() + # raise t, v, tb + # For some reason the above was giving a syntax error. + # A simple raise should reraise the existing exception. + raise + else: + TrinityPath, err_info = pipe1.communicate() + # FIX - probably should be checking err_info for errors... + # Determine the TRINITY_BASE_DIR from output1. + # If TrinityPath is a link, we need to dereference the link. + TrinityPath = TrinityPath.rstrip() # Need to strip off a newline. + # print "Trinity that was found is: {:s}".format(repr(TrinityPath)) + # print os.path.islink(TrinityPath) + TrinityPath = os.path.abspath(TrinityPath) + # msg = "The Absolute Trinity path that was found is: {:s}".format(TrinityPath) + # print msg + # print os.path.islink(TrinityPath) + while os.path.islink(TrinityPath): + # print "That path is a link." + TrinityPath = os.path.join(os.path.dirname(TrinityPath),os.readlink(TrinityPath)) + # print "The new path is: {:s}".format(TrinityPath) + # Take off the last part of the path (which is the Trinity command) + TRINITY_BASE_DIR = "/".join(TrinityPath.split("/")[0:-1]) +else: + sys.stderr.write("Either set TRINITY_HOME to the trinity base directory, or ensure that directory is in the PATH before running.") + sys.exit(1) + + +# get bindir +bindir = sys.argv[0] +bindir = bindir.split("/") +if len(bindir) > 1: + bindir.pop() + bindir = "/".join(bindir) +else: + bindir = "." + + +## add locations of tools to path setting. +#TOOL_PATHS_FILE = bindir + "/__add_to_PATH_setting.txt"; +#for line in open(TOOL_PATHS_FILE): +# line = line.rstrip() +# os.environ['PATH'] += ":" + line + +# Add TrinityPath and its utils to the PATH environment variable. +# print "Initially the PATH env variable is:\n\t{:s}".format(os.environ['PATH']) +os.environ['PATH'] = os.environ['PATH'] + ":{:s}:{:s}".format(TRINITY_BASE_DIR,TRINITY_BASE_DIR+"/util") +# print "Now the PATH env variable is:\n\t{:s}".format(os.environ['PATH']) + + +def stop_err( msg ): + sys.stderr.write( "%s\n" % msg ) + sys.exit() + +def __main__(): + # Get command-line arguments + args = sys.argv + # Remove name of calling program, i.e. ./stderr_wrapper.py + args.pop(0) + # If there are no arguments left, we're done + if len(args) == 0: + return + + # If one needs to silence stdout + #args.append( ">" ) + #args.append( "/dev/null" ) + print "The TRINITY_BASE_DIR is:\n\t{:s}".format(TRINITY_BASE_DIR) + print "The PATH env variable is:\n\t{:s}".format(os.environ['PATH']) + + args[0] = "".join([TRINITY_BASE_DIR, '/', args[0]]); + + cmdline = " ".join(args) + + print "The command being invoked is:\n\t{:s}".format(cmdline) + + + try: + # Run program + err_capture = open("stderr.txt", 'w') + proc = subprocess.Popen( args=cmdline, shell=True, stderr=err_capture, stdout=sys.stdout ) + returncode = proc.wait() + err_capture.close() + + + if returncode != 0: + raise Exception + + except Exception: + # Running Grinder failed: write error message to stderr + err_text = open("stderr.txt").readlines() + stop_err( "ERROR:\n" + "\n".join(err_text)) + + +if __name__ == "__main__": __main__()
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/Sp_ds.RSEM.genes.results Tue Jul 17 11:47:32 2018 -0400 @@ -0,0 +1,353 @@ +gene_id transcript_id(s) length effective_length expected_count TPM FPKM +TRINITY_DN100_c0_g1 TRINITY_DN100_c0_g1_i1 223.00 7.26 1.00 1618.59 1457.32 +TRINITY_DN100_c0_g2 TRINITY_DN100_c0_g2_i1 794.00 529.51 13.00 288.49 259.75 +TRINITY_DN101_c0_g1 TRINITY_DN101_c0_g1_i1 1450.00 1185.51 64.00 634.36 571.16 +TRINITY_DN101_c0_g2 TRINITY_DN101_c0_g2_i1 271.00 28.48 0.00 0.00 0.00 +TRINITY_DN101_c0_g3 TRINITY_DN101_c0_g3_i1 793.00 528.51 30.00 667.01 600.55 +TRINITY_DN102_c0_g1 TRINITY_DN102_c0_g1_i1 217.00 5.65 2.00 4156.55 3742.39 +TRINITY_DN104_c0_g1 TRINITY_DN104_c0_g1_i1 326.00 67.47 1.00 174.16 156.81 +TRINITY_DN104_c0_g2 TRINITY_DN104_c0_g2_i1 385.00 120.79 3.00 291.84 262.76 +TRINITY_DN105_c0_g1 TRINITY_DN105_c0_g1_i1 569.00 304.51 7.00 270.12 243.21 +TRINITY_DN105_c0_g2 TRINITY_DN105_c0_g2_i1 1004.00 739.51 16.00 254.24 228.91 +TRINITY_DN106_c0_g1 TRINITY_DN106_c0_g1_i1 818.00 553.51 29.00 615.65 554.31 +TRINITY_DN107_c0_g1 TRINITY_DN107_c0_g1_i1 564.00 299.51 13.00 510.03 459.21 +TRINITY_DN108_c0_g1 TRINITY_DN108_c0_g1_i1 300.00 47.43 1.00 247.77 223.08 +TRINITY_DN108_c0_g2 TRINITY_DN108_c0_g2_i1 735.00 470.51 8.00 199.79 179.89 +TRINITY_DN109_c0_g1 TRINITY_DN109_c0_g1_i1,TRINITY_DN109_c0_g1_i2 1120.33 855.84 55.00 755.15 679.91 +TRINITY_DN10_c0_g1 TRINITY_DN10_c0_g1_i1 735.00 470.51 97.00 2422.51 2181.14 +TRINITY_DN110_c0_g1 TRINITY_DN110_c0_g1_i1 1194.00 929.51 644.00 8141.33 7330.14 +TRINITY_DN111_c0_g1 TRINITY_DN111_c0_g1_i1 263.00 23.97 6.00 2941.61 2648.52 +TRINITY_DN111_c0_g2 TRINITY_DN111_c0_g2_i1 371.00 107.34 13.00 1423.20 1281.39 +TRINITY_DN112_c0_g1 TRINITY_DN112_c0_g1_i1 370.00 106.39 2.00 220.90 198.89 +TRINITY_DN113_c0_g1 TRINITY_DN113_c0_g1_i1 503.00 238.51 12.00 591.20 532.30 +TRINITY_DN115_c0_g1 TRINITY_DN115_c0_g1_i1 989.00 724.51 28.00 454.13 408.88 +TRINITY_DN115_c0_g2 TRINITY_DN115_c0_g2_i1 513.00 248.51 9.00 425.56 383.16 +TRINITY_DN116_c0_g1 TRINITY_DN116_c0_g1_i1 354.00 91.62 2.00 256.51 230.95 +TRINITY_DN116_c0_g2 TRINITY_DN116_c0_g2_i1 260.00 22.37 2.00 1050.69 946.00 +TRINITY_DN116_c0_g3 TRINITY_DN116_c0_g3_i1 219.00 6.16 1.00 1907.25 1717.21 +TRINITY_DN117_c0_g1 TRINITY_DN117_c0_g1_i1 208.00 3.71 0.00 0.00 0.00 +TRINITY_DN117_c0_g2 TRINITY_DN117_c0_g2_i1 235.00 11.19 0.00 0.00 0.00 +TRINITY_DN117_c0_g3 TRINITY_DN117_c0_g3_i1 345.00 83.60 4.00 562.20 506.19 +TRINITY_DN117_c0_g4 TRINITY_DN117_c0_g4_i1 276.00 31.46 1.00 373.50 336.29 +TRINITY_DN117_c0_g5 TRINITY_DN117_c0_g5_i1 560.00 295.51 9.00 357.88 322.22 +TRINITY_DN118_c0_g1 TRINITY_DN118_c0_g1_i1 450.00 185.51 7.00 443.40 399.22 +TRINITY_DN118_c0_g2 TRINITY_DN118_c0_g2_i1 242.00 13.93 2.00 1687.41 1519.28 +TRINITY_DN118_c0_g3 TRINITY_DN118_c0_g3_i1 207.00 3.53 0.00 0.00 0.00 +TRINITY_DN118_c0_g4 TRINITY_DN118_c0_g4_i1 228.00 8.78 0.00 0.00 0.00 +TRINITY_DN118_c0_g5 TRINITY_DN118_c0_g5_i1 303.00 49.60 2.00 473.79 426.59 +TRINITY_DN119_c0_g1 TRINITY_DN119_c0_g1_i1 316.00 59.46 0.00 0.00 0.00 +TRINITY_DN119_c0_g2 TRINITY_DN119_c0_g2_i1 529.00 264.51 5.00 222.12 199.99 +TRINITY_DN119_c0_g3 TRINITY_DN119_c0_g3_i1 403.00 138.57 0.00 0.00 0.00 +TRINITY_DN11_c0_g1 TRINITY_DN11_c0_g1_i1 311.00 55.59 6.00 1268.37 1142.00 +TRINITY_DN120_c0_g1 TRINITY_DN120_c0_g1_i1 245.00 15.19 1.00 773.34 696.28 +TRINITY_DN120_c0_g2 TRINITY_DN120_c0_g2_i1 310.00 54.82 0.00 0.00 0.00 +TRINITY_DN120_c0_g3 TRINITY_DN120_c0_g3_i1 976.00 711.51 20.00 330.30 297.39 +TRINITY_DN121_c0_g1 TRINITY_DN121_c0_g1_i1 1780.00 1515.51 196.00 1519.71 1368.29 +TRINITY_DN122_c0_g1 TRINITY_DN122_c0_g1_i1 1415.00 1150.51 32.00 326.83 294.27 +TRINITY_DN122_c0_g2 TRINITY_DN122_c0_g2_i1 459.00 194.51 6.00 362.47 326.35 +TRINITY_DN123_c0_g1 TRINITY_DN123_c0_g1_i1 203.00 2.87 0.00 0.00 0.00 +TRINITY_DN123_c0_g2 TRINITY_DN123_c0_g2_i1 302.00 48.87 4.00 961.74 865.91 +TRINITY_DN124_c0_g1 TRINITY_DN124_c0_g1_i1 519.00 254.51 19.00 877.23 789.82 +TRINITY_DN124_c0_g2 TRINITY_DN124_c0_g2_i1 377.00 113.05 13.00 1351.26 1216.63 +TRINITY_DN124_c0_g3 TRINITY_DN124_c0_g3_i1 1509.00 1244.51 93.00 878.11 790.62 +TRINITY_DN124_c0_g4 TRINITY_DN124_c0_g4_i1 1041.00 776.51 49.00 741.50 667.62 +TRINITY_DN125_c0_g1 TRINITY_DN125_c0_g1_i1 319.00 61.82 3.00 570.22 513.41 +TRINITY_DN126_c0_g1 TRINITY_DN126_c0_g1_i1 557.00 292.51 4.00 160.69 144.68 +TRINITY_DN126_c0_g2 TRINITY_DN126_c0_g2_i1 596.00 331.51 7.00 248.12 223.40 +TRINITY_DN126_c0_g3 TRINITY_DN126_c0_g3_i1 811.00 546.51 14.00 301.02 271.03 +TRINITY_DN127_c0_g1 TRINITY_DN127_c0_g1_i1 1294.00 1029.51 47.00 536.45 483.00 +TRINITY_DN127_c0_g2 TRINITY_DN127_c0_g2_i1 281.00 34.57 10.00 3399.59 3060.86 +TRINITY_DN128_c0_g1 TRINITY_DN128_c0_g1_i1 3008.00 2743.51 1724.00 7384.05 6648.31 +TRINITY_DN128_c1_g1 TRINITY_DN128_c1_g1_i1,TRINITY_DN128_c1_g1_i2,TRINITY_DN128_c1_g1_i3,TRINITY_DN128_c1_g1_i4 2111.00 1846.51 5325.00 33886.88 30510.45 +TRINITY_DN129_c0_g1 TRINITY_DN129_c0_g1_i1 1646.00 1381.51 146.00 1241.83 1118.10 +TRINITY_DN130_c0_g1 TRINITY_DN130_c0_g1_i1 838.00 573.51 43.00 881.03 793.25 +TRINITY_DN130_c0_g2 TRINITY_DN130_c0_g2_i1 909.00 644.51 44.00 802.21 722.28 +TRINITY_DN132_c0_g1 TRINITY_DN132_c0_g1_i1 212.00 4.51 0.00 0.00 0.00 +TRINITY_DN132_c0_g2 TRINITY_DN132_c0_g2_i1 281.00 34.57 3.00 1019.88 918.26 +TRINITY_DN133_c0_g1 TRINITY_DN133_c0_g1_i1 550.00 285.51 12.00 493.88 444.67 +TRINITY_DN133_c0_g2 TRINITY_DN133_c0_g2_i1 340.00 79.25 4.00 593.08 533.99 +TRINITY_DN133_c0_g3 TRINITY_DN133_c0_g3_i1 227.00 8.46 0.00 0.00 0.00 +TRINITY_DN134_c0_g1 TRINITY_DN134_c0_g1_i1 487.00 222.51 8.00 422.48 380.38 +TRINITY_DN135_c0_g1 TRINITY_DN135_c0_g1_i1 276.00 31.46 3.00 1120.51 1008.86 +TRINITY_DN135_c0_g2 TRINITY_DN135_c0_g2_i1 411.00 146.54 6.00 481.13 433.19 +TRINITY_DN135_c0_g3 TRINITY_DN135_c0_g3_i1 239.00 12.72 0.00 0.00 0.00 +TRINITY_DN136_c0_g1 TRINITY_DN136_c0_g1_i1 635.00 370.51 7.00 222.00 199.88 +TRINITY_DN137_c0_g1 TRINITY_DN137_c0_g1_i1 238.00 12.33 0.00 0.00 0.00 +TRINITY_DN138_c0_g1 TRINITY_DN138_c0_g1_i1 338.00 77.53 4.00 606.24 545.84 +TRINITY_DN13_c0_g1 TRINITY_DN13_c0_g1_i1 1398.00 1133.51 495.00 5131.49 4620.20 +TRINITY_DN140_c0_g1 TRINITY_DN140_c0_g1_i1 299.00 46.71 3.00 754.72 679.52 +TRINITY_DN141_c0_g1 TRINITY_DN141_c0_g1_i1 217.00 5.65 0.00 0.00 0.00 +TRINITY_DN141_c0_g2 TRINITY_DN141_c0_g2_i1 425.00 160.52 6.00 439.23 395.47 +TRINITY_DN141_c0_g3 TRINITY_DN141_c0_g3_i1 394.00 129.64 3.00 271.93 244.84 +TRINITY_DN142_c0_g1 TRINITY_DN142_c0_g1_i1 1024.00 759.51 100.00 1547.14 1392.99 +TRINITY_DN143_c0_g1 TRINITY_DN143_c0_g1_i1 374.00 110.18 5.00 533.24 480.11 +TRINITY_DN143_c0_g2 TRINITY_DN143_c0_g2_i1 255.00 19.82 2.00 1185.70 1067.56 +TRINITY_DN143_c0_g3 TRINITY_DN143_c0_g3_i1 497.00 232.51 5.00 252.69 227.51 +TRINITY_DN143_c0_g4 TRINITY_DN143_c0_g4_i1 1066.00 801.51 32.00 469.14 422.40 +TRINITY_DN143_c0_g5 TRINITY_DN143_c0_g5_i1 291.00 41.13 1.00 285.70 257.23 +TRINITY_DN143_c0_g6 TRINITY_DN143_c0_g6_i1 410.00 145.54 3.00 242.21 218.08 +TRINITY_DN144_c0_g1 TRINITY_DN144_c0_g1_i1 202.00 2.72 0.00 0.00 0.00 +TRINITY_DN144_c0_g2 TRINITY_DN144_c0_g2_i1 388.00 123.73 4.00 379.89 342.04 +TRINITY_DN144_c0_g3 TRINITY_DN144_c0_g3_i1 204.00 3.03 0.00 0.00 0.00 +TRINITY_DN145_c0_g1 TRINITY_DN145_c0_g1_i1 570.00 305.51 6.00 230.77 207.78 +TRINITY_DN146_c0_g1 TRINITY_DN146_c0_g1_i1 430.00 165.51 4.00 283.98 255.69 +TRINITY_DN146_c0_g2 TRINITY_DN146_c0_g2_i1 580.00 315.51 13.00 484.16 435.92 +TRINITY_DN146_c0_g3 TRINITY_DN146_c0_g3_i1 605.00 340.51 21.00 724.69 652.48 +TRINITY_DN146_c0_g4 TRINITY_DN146_c0_g4_i1 1025.00 760.51 31.00 478.98 431.26 +TRINITY_DN149_c0_g1 TRINITY_DN149_c0_g1_i1 322.00 64.22 1.00 182.97 164.74 +TRINITY_DN149_c0_g2 TRINITY_DN149_c0_g2_i1 673.00 408.51 22.00 632.82 569.77 +TRINITY_DN149_c0_g3 TRINITY_DN149_c0_g3_i1 492.00 227.51 8.00 413.19 372.02 +TRINITY_DN149_c0_g4 TRINITY_DN149_c0_g4_i1 234.00 10.83 1.00 1085.16 977.04 +TRINITY_DN149_c0_g5 TRINITY_DN149_c0_g5_i1 286.00 37.79 2.00 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1300.00 12526.26 11278.17 +TRINITY_DN211_c0_g1 TRINITY_DN211_c0_g1_i1 429.00 164.51 53.00 3785.60 3408.41 +TRINITY_DN212_c0_g1 TRINITY_DN212_c0_g1_i1 826.00 561.51 59.00 1234.69 1111.67 +TRINITY_DN213_c0_g1 TRINITY_DN213_c0_g1_i1 1471.00 1206.51 95.00 925.24 833.05 +TRINITY_DN214_c0_g1 TRINITY_DN214_c0_g1_i1 755.00 490.51 31.00 742.64 668.64 +TRINITY_DN215_c0_g1 TRINITY_DN215_c0_g1_i1 281.00 34.57 29.00 9858.80 8876.49 +TRINITY_DN216_c0_g1 TRINITY_DN216_c0_g1_i1 1965.00 1700.51 3763.00 26002.72 23411.85 +TRINITY_DN217_c0_g1 TRINITY_DN217_c0_g1_i1 504.00 239.51 7.00 343.43 309.21 +TRINITY_DN218_c0_g1 TRINITY_DN218_c0_g1_i1 456.00 191.51 7.00 429.51 386.71 +TRINITY_DN219_c0_g1 TRINITY_DN219_c0_g1_i1 709.00 444.51 74.00 1956.20 1761.29 +TRINITY_DN21_c0_g1 TRINITY_DN21_c0_g1_i1 1787.00 1522.51 109.00 841.26 757.44 +TRINITY_DN220_c0_g1 TRINITY_DN220_c0_g1_i1 208.00 3.71 0.00 0.00 0.00 +TRINITY_DN221_c0_g1 TRINITY_DN221_c0_g1_i1 2581.00 2316.51 2084.00 10571.27 9517.97 +TRINITY_DN222_c0_g1 TRINITY_DN222_c0_g1_i1 4108.00 3843.51 10596.00 32394.98 29167.20 +TRINITY_DN223_c0_g1 TRINITY_DN223_c0_g1_i1 1222.00 957.51 341.00 4184.80 3767.83 +TRINITY_DN224_c0_g1 TRINITY_DN224_c0_g1_i1 2133.00 1868.51 100.00 628.88 566.22 +TRINITY_DN225_c0_g1 TRINITY_DN225_c0_g1_i1 2344.00 2079.51 892.00 5040.43 4538.21 +TRINITY_DN226_c0_g1 TRINITY_DN226_c0_g1_i1 503.00 238.51 636.00 31333.78 28211.74 +TRINITY_DN227_c0_g1 TRINITY_DN227_c0_g1_i1 1072.00 807.51 44.00 640.28 576.48 +TRINITY_DN228_c0_g1 TRINITY_DN228_c0_g1_i1 1246.00 981.51 1686.00 20184.89 18173.70 +TRINITY_DN229_c0_g1 TRINITY_DN229_c0_g1_i1 1114.00 849.51 160.00 2213.17 1992.65 +TRINITY_DN22_c0_g1 TRINITY_DN22_c0_g1_i1 2102.00 1837.51 444.00 2839.34 2556.43 +TRINITY_DN230_c0_g1 TRINITY_DN230_c0_g1_i1 679.00 414.51 490.00 13890.70 12506.65 +TRINITY_DN231_c0_g1 TRINITY_DN231_c0_g1_i1 1561.00 1296.51 826.00 7486.31 6740.39 +TRINITY_DN232_c0_g1 TRINITY_DN232_c0_g1_i1 1930.00 1665.51 275.00 1940.21 1746.89 +TRINITY_DN23_c0_g1 TRINITY_DN23_c0_g1_i1 1197.00 932.51 112.00 1411.33 1270.71 +TRINITY_DN24_c0_g1 TRINITY_DN24_c0_g1_i1 276.00 31.46 1.00 373.50 336.29 +TRINITY_DN24_c0_g2 TRINITY_DN24_c0_g2_i1 356.00 93.43 7.00 880.38 792.66 +TRINITY_DN25_c0_g1 TRINITY_DN25_c0_g1_i1 2494.00 2229.51 99.00 521.78 469.79 +TRINITY_DN26_c0_g1 TRINITY_DN26_c0_g1_i1 946.00 681.51 9.00 155.18 139.72 +TRINITY_DN26_c0_g2 TRINITY_DN26_c0_g2_i1 434.00 169.51 12.00 831.85 748.96 +TRINITY_DN27_c0_g1 TRINITY_DN27_c0_g1_i1 317.00 60.24 3.00 585.19 526.88 +TRINITY_DN28_c0_g1 TRINITY_DN28_c0_g1_i1 1235.00 970.51 217.00 2627.38 2365.59 +TRINITY_DN29_c0_g1 TRINITY_DN29_c0_g1_i1 515.00 250.51 9.00 422.16 380.10 +TRINITY_DN30_c0_g1 TRINITY_DN30_c0_g1_i1 309.00 54.07 0.00 0.00 0.00 +TRINITY_DN31_c0_g1 TRINITY_DN31_c0_g1_i1,TRINITY_DN31_c0_g1_i2 2191.03 1926.54 406.00 2476.35 2229.61 +TRINITY_DN32_c0_g1 TRINITY_DN32_c0_g1_i1 1795.00 1530.51 181.00 1389.65 1251.19 +TRINITY_DN33_c0_g1 TRINITY_DN33_c0_g1_i1 314.00 57.90 8.00 1623.71 1461.93 +TRINITY_DN35_c0_g1 TRINITY_DN35_c0_g1_i1 740.00 475.51 292.00 7215.83 6496.86 +TRINITY_DN36_c0_g1 TRINITY_DN36_c0_g1_i1 307.00 52.56 2.00 447.11 402.56 +TRINITY_DN38_c0_g1 TRINITY_DN38_c0_g1_i1 2369.00 2104.51 161.00 898.96 809.39 +TRINITY_DN3_c0_g1 TRINITY_DN3_c0_g1_i1 802.00 537.51 98.00 2142.41 1928.94 +TRINITY_DN40_c0_g1 TRINITY_DN40_c0_g1_i1 1559.00 1294.51 72.00 653.57 588.45 +TRINITY_DN41_c0_g1 TRINITY_DN41_c0_g1_i1 788.00 523.51 15.00 336.69 303.14 +TRINITY_DN43_c0_g1 TRINITY_DN43_c0_g1_i1 1539.00 1274.51 81.00 746.80 672.39 +TRINITY_DN45_c0_g1 TRINITY_DN45_c0_g1_i1 1141.00 876.51 199.00 2667.84 2402.02 +TRINITY_DN46_c0_g1 TRINITY_DN46_c0_g1_i1 335.00 74.97 2.00 313.47 282.23 +TRINITY_DN46_c0_g2 TRINITY_DN46_c0_g2_i1 318.00 61.03 2.00 385.08 346.71 +TRINITY_DN47_c0_g1 TRINITY_DN47_c0_g1_i1 982.00 717.51 94.00 1539.44 1386.05 +TRINITY_DN48_c0_g1 TRINITY_DN48_c0_g1_i1 1959.00 1694.51 174.00 1206.62 1086.39 +TRINITY_DN49_c0_g1 TRINITY_DN49_c0_g1_i1 1674.00 1409.51 58.00 483.53 435.35 +TRINITY_DN4_c0_g1 TRINITY_DN4_c0_g1_i1 355.00 92.52 11.00 1397.02 1257.82 +TRINITY_DN4_c0_g2 TRINITY_DN4_c0_g2_i1 2725.00 2460.51 97.00 463.24 417.09 +TRINITY_DN50_c0_g1 TRINITY_DN50_c0_g1_i1 1317.00 1052.51 10195.00 113821.56 102480.57 +TRINITY_DN51_c0_g1 TRINITY_DN51_c0_g1_i1 1002.00 737.51 18.00 286.79 258.22 +TRINITY_DN52_c0_g1 TRINITY_DN52_c0_g1_i1 1868.00 1603.51 140.00 1025.94 923.71 +TRINITY_DN53_c0_g1 TRINITY_DN53_c0_g1_i1 918.00 653.51 43.00 773.18 696.14 +TRINITY_DN53_c0_g2 TRINITY_DN53_c0_g2_i1 3790.00 3525.51 314.00 1046.58 942.30 +TRINITY_DN54_c0_g1 TRINITY_DN54_c0_g1_i1 1028.00 763.51 20.00 307.81 277.14 +TRINITY_DN55_c0_g1 TRINITY_DN55_c0_g1_i1 1086.00 821.51 45.00 643.67 579.54 +TRINITY_DN57_c0_g1 TRINITY_DN57_c0_g1_i1 223.00 7.26 2.00 3237.18 2914.63 +TRINITY_DN58_c0_g1 TRINITY_DN58_c0_g1_i1 851.00 586.51 90.00 1803.14 1623.48 +TRINITY_DN59_c0_g1 TRINITY_DN59_c0_g1_i1 243.00 14.34 1.00 819.22 737.60 +TRINITY_DN5_c0_g1 TRINITY_DN5_c0_g1_i1 1281.00 1016.51 101.00 1167.54 1051.21 +TRINITY_DN60_c0_g1 TRINITY_DN60_c0_g1_i1 447.00 182.51 70.00 4506.85 4057.79 +TRINITY_DN60_c0_g2 TRINITY_DN60_c0_g2_i1 232.00 10.12 0.00 0.00 0.00 +TRINITY_DN62_c0_g1 TRINITY_DN62_c0_g1_i1 494.00 229.51 10.00 511.99 460.98 +TRINITY_DN62_c0_g2 TRINITY_DN62_c0_g2_i1 325.00 66.65 0.00 0.00 0.00 +TRINITY_DN63_c0_g1 TRINITY_DN63_c0_g1_i1 1405.00 1140.51 328.00 3379.39 3042.67 +TRINITY_DN64_c0_g1 TRINITY_DN64_c0_g1_i1 4840.00 4575.51 379.00 973.34 876.36 +TRINITY_DN66_c0_g1 TRINITY_DN66_c0_g1_i1 223.00 7.26 0.00 0.00 0.00 +TRINITY_DN66_c0_g2 TRINITY_DN66_c0_g2_i1 1392.00 1127.51 272.00 2834.73 2552.28 +TRINITY_DN67_c0_g1 TRINITY_DN67_c0_g1_i1 270.00 27.90 2.00 842.45 758.51 +TRINITY_DN68_c0_g1 TRINITY_DN68_c0_g1_i1 829.00 564.51 50.00 1040.79 937.08 +TRINITY_DN69_c0_g1 TRINITY_DN69_c0_g1_i1 381.00 116.90 5.00 502.58 452.50 +TRINITY_DN70_c0_g1 TRINITY_DN70_c0_g1_i1 216.00 5.41 0.00 0.00 0.00 +TRINITY_DN70_c0_g2 TRINITY_DN70_c0_g2_i1 496.00 231.51 9.00 456.81 411.29 +TRINITY_DN71_c0_g1 TRINITY_DN71_c0_g1_i1 247.00 16.07 0.00 0.00 0.00 +TRINITY_DN71_c0_g2 TRINITY_DN71_c0_g2_i1 1598.00 1333.51 191.00 1683.06 1515.37 +TRINITY_DN72_c0_g1 TRINITY_DN72_c0_g1_i1 348.00 86.25 7.00 953.68 858.65 +TRINITY_DN74_c0_g1 TRINITY_DN74_c0_g1_i1 965.00 700.51 25.00 419.36 377.58 +TRINITY_DN75_c0_g1 TRINITY_DN75_c0_g1_i1 266.00 25.62 2.00 917.31 825.91 +TRINITY_DN75_c0_g2 TRINITY_DN75_c0_g2_i1 202.00 2.72 0.00 0.00 0.00 +TRINITY_DN76_c0_g1 TRINITY_DN76_c0_g1_i1 525.00 260.51 14.00 631.49 568.57 +TRINITY_DN76_c0_g2 TRINITY_DN76_c0_g2_i1 379.00 114.97 4.00 408.82 368.08 +TRINITY_DN76_c0_g3 TRINITY_DN76_c0_g3_i1 1516.00 1251.51 55.00 516.41 464.95 +TRINITY_DN77_c0_g1 TRINITY_DN77_c0_g1_i1 220.00 6.43 0.00 0.00 0.00 +TRINITY_DN77_c0_g2 TRINITY_DN77_c0_g2_i1 265.00 25.06 0.00 0.00 0.00 +TRINITY_DN78_c0_g1 TRINITY_DN78_c0_g1_i1 207.00 3.53 0.00 0.00 0.00 +TRINITY_DN78_c0_g2 TRINITY_DN78_c0_g2_i1 1720.00 1455.51 77.00 621.64 559.70 +TRINITY_DN78_c0_g3 TRINITY_DN78_c0_g3_i1 721.00 456.51 17.00 437.58 393.98 +TRINITY_DN79_c0_g1 TRINITY_DN79_c0_g1_i1 587.00 322.51 7.00 255.05 229.63 +TRINITY_DN80_c0_g1 TRINITY_DN80_c0_g1_i1 319.00 61.82 1.00 190.07 171.14 +TRINITY_DN81_c0_g1 TRINITY_DN81_c0_g1_i1 1289.00 1024.51 143.00 1640.15 1476.73 +TRINITY_DN81_c0_g2 TRINITY_DN81_c0_g2_i1 1182.00 917.51 115.00 1472.82 1326.07 +TRINITY_DN82_c0_g1 TRINITY_DN82_c0_g1_i1 1440.00 1175.51 86.00 859.68 774.02 +TRINITY_DN83_c0_g1 TRINITY_DN83_c0_g1_i1 212.00 4.51 0.00 0.00 0.00 +TRINITY_DN83_c0_g2 TRINITY_DN83_c0_g2_i1 281.00 34.57 2.00 679.92 612.17 +TRINITY_DN84_c0_g1 TRINITY_DN84_c0_g1_i1 362.00 98.93 1.00 118.78 106.95 +TRINITY_DN85_c0_g1 TRINITY_DN85_c0_g1_i1 626.00 361.51 23.00 747.60 673.11 +TRINITY_DN85_c0_g2 TRINITY_DN85_c0_g2_i1 1045.00 780.51 46.00 692.54 623.53 +TRINITY_DN86_c0_g1 TRINITY_DN86_c0_g1_i1 298.00 46.00 0.00 0.00 0.00 +TRINITY_DN86_c0_g2 TRINITY_DN86_c0_g2_i1 332.00 72.44 1.00 162.21 146.05 +TRINITY_DN86_c0_g3 TRINITY_DN86_c0_g3_i1 285.00 37.13 1.00 316.44 284.91 +TRINITY_DN87_c0_g1 TRINITY_DN87_c0_g1_i1 267.00 26.18 2.00 897.64 808.20 +TRINITY_DN89_c0_g1 TRINITY_DN89_c0_g1_i1 259.00 21.85 0.00 0.00 0.00 +TRINITY_DN89_c0_g2 TRINITY_DN89_c0_g2_i1 797.00 532.51 15.00 331.00 298.02 +TRINITY_DN89_c0_g3 TRINITY_DN89_c0_g3_i1 232.00 10.12 0.00 0.00 0.00 +TRINITY_DN8_c0_g1 TRINITY_DN8_c0_g1_i1 2016.00 1751.51 587.00 3938.12 3545.73 +TRINITY_DN90_c0_g1 TRINITY_DN90_c0_g1_i1 456.00 191.51 5.00 306.79 276.22 +TRINITY_DN91_c0_g1 TRINITY_DN91_c0_g1_i1 1374.00 1109.51 40.00 423.64 381.43 +TRINITY_DN92_c0_g1 TRINITY_DN92_c0_g1_i1 1030.00 765.51 1154.00 17714.07 15949.07 +TRINITY_DN93_c0_g1 TRINITY_DN93_c0_g1_i1 237.00 11.94 0.00 0.00 0.00 +TRINITY_DN93_c0_g2 TRINITY_DN93_c0_g2_i1 249.00 16.97 0.00 0.00 0.00 +TRINITY_DN93_c0_g3 TRINITY_DN93_c0_g3_i1 660.00 395.51 18.00 534.78 481.50 +TRINITY_DN94_c0_g1 TRINITY_DN94_c0_g1_i1 381.00 116.90 3.00 301.55 271.50 +TRINITY_DN95_c0_g1 TRINITY_DN95_c0_g1_i1 407.00 142.55 4.00 329.73 296.87 +TRINITY_DN95_c0_g2 TRINITY_DN95_c0_g2_i1 614.00 349.51 16.00 537.93 484.33 +TRINITY_DN95_c0_g3 TRINITY_DN95_c0_g3_i1 264.00 24.51 2.00 958.73 863.21 +TRINITY_DN96_c0_g1 TRINITY_DN96_c0_g1_i1 652.00 387.51 29.00 879.38 791.76 +TRINITY_DN97_c0_g1 TRINITY_DN97_c0_g1_i1 500.00 235.51 31.00 1546.73 1392.62 +TRINITY_DN97_c0_g2 TRINITY_DN97_c0_g2_i1 1352.00 1087.51 82.00 886.02 797.74 +TRINITY_DN98_c0_g1 TRINITY_DN98_c0_g1_i1 499.00 234.51 10.00 501.07 451.15 +TRINITY_DN99_c0_g1 TRINITY_DN99_c0_g1_i1 960.00 695.51 32.00 540.64 486.77 +TRINITY_DN99_c0_g2 TRINITY_DN99_c0_g2_i1 201.00 2.58 0.00 0.00 0.00 +TRINITY_DN9_c0_g1 TRINITY_DN9_c0_g1_i1 270.00 27.90 1.00 421.22 379.25
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/Sp_hs.RSEM.genes.results Tue Jul 17 11:47:32 2018 -0400 @@ -0,0 +1,353 @@ +gene_id transcript_id(s) length effective_length expected_count TPM FPKM +TRINITY_DN100_c0_g1 TRINITY_DN100_c0_g1_i1 223.00 3.02 1.00 3639.81 3284.97 +TRINITY_DN100_c0_g2 TRINITY_DN100_c0_g2_i1 794.00 513.05 21.00 449.31 405.51 +TRINITY_DN101_c0_g1 TRINITY_DN101_c0_g1_i1 1450.00 1169.05 56.00 525.82 474.56 +TRINITY_DN101_c0_g2 TRINITY_DN101_c0_g2_i1 271.00 19.35 0.00 0.00 0.00 +TRINITY_DN101_c0_g3 TRINITY_DN101_c0_g3_i1 793.00 512.05 19.00 407.31 367.60 +TRINITY_DN102_c0_g1 TRINITY_DN102_c0_g1_i1 217.00 2.11 0.00 0.00 0.00 +TRINITY_DN104_c0_g1 TRINITY_DN104_c0_g1_i1 326.00 54.66 5.00 1004.09 906.20 +TRINITY_DN104_c0_g2 TRINITY_DN104_c0_g2_i1 385.00 105.12 9.00 939.81 848.19 +TRINITY_DN105_c0_g1 TRINITY_DN105_c0_g1_i1 569.00 288.05 8.00 304.87 275.15 +TRINITY_DN105_c0_g2 TRINITY_DN105_c0_g2_i1 1004.00 723.05 26.00 394.72 356.24 +TRINITY_DN106_c0_g1 TRINITY_DN106_c0_g1_i1 818.00 537.05 19.00 388.35 350.49 +TRINITY_DN107_c0_g1 TRINITY_DN107_c0_g1_i1 564.00 283.05 7.00 271.47 245.01 +TRINITY_DN108_c0_g1 TRINITY_DN108_c0_g1_i1 300.00 36.21 0.00 0.00 0.00 +TRINITY_DN108_c0_g2 TRINITY_DN108_c0_g2_i1 735.00 454.05 18.00 435.17 392.74 +TRINITY_DN109_c0_g1 TRINITY_DN109_c0_g1_i1,TRINITY_DN109_c0_g1_i2 1117.82 836.86 212.00 2780.78 2509.68 +TRINITY_DN10_c0_g1 TRINITY_DN10_c0_g1_i1 735.00 454.05 36.00 870.34 785.49 +TRINITY_DN110_c0_g1 TRINITY_DN110_c0_g1_i1 1194.00 913.05 227.00 2729.09 2463.03 +TRINITY_DN111_c0_g1 TRINITY_DN111_c0_g1_i1 263.00 15.51 0.00 0.00 0.00 +TRINITY_DN111_c0_g2 TRINITY_DN111_c0_g2_i1 371.00 92.23 0.00 0.00 0.00 +TRINITY_DN112_c0_g1 TRINITY_DN112_c0_g1_i1 370.00 91.33 1.00 120.20 108.48 +TRINITY_DN113_c0_g1 TRINITY_DN113_c0_g1_i1 503.00 222.05 6.00 296.61 267.70 +TRINITY_DN115_c0_g1 TRINITY_DN115_c0_g1_i1 989.00 708.05 25.00 387.58 349.80 +TRINITY_DN115_c0_g2 TRINITY_DN115_c0_g2_i1 513.00 232.05 4.00 189.22 170.77 +TRINITY_DN116_c0_g1 TRINITY_DN116_c0_g1_i1 354.00 77.32 1.00 141.97 128.13 +TRINITY_DN116_c0_g2 TRINITY_DN116_c0_g2_i1 260.00 14.18 0.00 0.00 0.00 +TRINITY_DN116_c0_g3 TRINITY_DN116_c0_g3_i1 219.00 2.38 0.00 0.00 0.00 +TRINITY_DN117_c0_g1 TRINITY_DN117_c0_g1_i1 208.00 1.24 0.00 0.00 0.00 +TRINITY_DN117_c0_g2 TRINITY_DN117_c0_g2_i1 235.00 5.61 2.00 3914.29 3532.69 +TRINITY_DN117_c0_g3 TRINITY_DN117_c0_g3_i1 345.00 69.76 2.00 314.69 284.01 +TRINITY_DN117_c0_g4 TRINITY_DN117_c0_g4_i1 276.00 21.95 1.00 500.14 451.38 +TRINITY_DN117_c0_g5 TRINITY_DN117_c0_g5_i1 560.00 279.05 11.00 432.71 390.53 +TRINITY_DN118_c0_g1 TRINITY_DN118_c0_g1_i1 450.00 169.05 4.00 259.73 234.41 +TRINITY_DN118_c0_g2 TRINITY_DN118_c0_g2_i1 242.00 7.58 0.00 0.00 0.00 +TRINITY_DN118_c0_g3 TRINITY_DN118_c0_g3_i1 207.00 1.18 0.00 0.00 0.00 +TRINITY_DN118_c0_g4 TRINITY_DN118_c0_g4_i1 228.00 3.97 0.00 0.00 0.00 +TRINITY_DN118_c0_g5 TRINITY_DN118_c0_g5_i1 303.00 38.19 0.00 0.00 0.00 +TRINITY_DN119_c0_g1 TRINITY_DN119_c0_g1_i1 316.00 47.23 4.00 929.65 839.02 +TRINITY_DN119_c0_g2 TRINITY_DN119_c0_g2_i1 529.00 248.05 12.00 531.04 479.27 +TRINITY_DN119_c0_g3 TRINITY_DN119_c0_g3_i1 403.00 122.36 1.00 89.71 80.97 +TRINITY_DN11_c0_g1 TRINITY_DN11_c0_g1_i1 311.00 43.67 10.00 2513.77 2268.70 +TRINITY_DN120_c0_g1 TRINITY_DN120_c0_g1_i1 245.00 8.53 0.00 0.00 0.00 +TRINITY_DN120_c0_g2 TRINITY_DN120_c0_g2_i1 310.00 42.97 1.00 255.47 230.57 +TRINITY_DN120_c0_g3 TRINITY_DN120_c0_g3_i1 976.00 695.05 15.00 236.90 213.80 +TRINITY_DN121_c0_g1 TRINITY_DN121_c0_g1_i1 1780.00 1499.05 234.00 1713.50 1546.46 +TRINITY_DN122_c0_g1 TRINITY_DN122_c0_g1_i1 1415.00 1134.05 16.00 154.87 139.77 +TRINITY_DN122_c0_g2 TRINITY_DN122_c0_g2_i1 459.00 178.05 1.00 61.65 55.64 +TRINITY_DN123_c0_g1 TRINITY_DN123_c0_g1_i1 203.00 0.00 0.00 0.00 0.00 +TRINITY_DN123_c0_g2 TRINITY_DN123_c0_g2_i1 302.00 37.53 0.00 0.00 0.00 +TRINITY_DN124_c0_g1 TRINITY_DN124_c0_g1_i1 519.00 238.05 7.00 322.79 291.32 +TRINITY_DN124_c0_g2 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