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author | trinity_ctat |
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date | Tue, 17 Jul 2018 11:52:28 -0400 |
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<tool id="ctat_gmap_fusion" name="ctat_gmap_fusion" version="1.0.0" profile="17.05"> <description>Fusion-finding Using De novo RNA-Seq Transcript Assemblies</description> <requirements> <requirement type="package" version="0.4.0">gmap-fusion</requirement> </requirements> <command detect_errors="default"> GMAP-fusion -T $assembled_trans --left_fq $left_input --right_fq $right_input --genome_lib_dir "${genome_resource_lib.fields.path}" --output "subdir" </command> <stdio> <exit_code range="1:" level="fatal" description="Error returned from pipeline" /> </stdio> <regex match="Must investigate error above." source="stderr" level="fatal" description="Unknown error encountered" /> <inputs> <param format="fasta" name="assembled_trans" type="data" label="Assembled Transcripts (ie. Trinity or DISCASM output)" help="Trinity or DISCASM generated transcript fasta"/> <param format="fastq" name="left_input" type="data" label="Left/Forward strand reads" help="Forward strand reads"/> <param format="fastq" name="right_input" type="data" label="Right/Reverse strand reads" help="Reverse strand reads"/> <param name="genome_resource_lib" type="select" label="Select a reference genome"> <options from_data_table="ctat_genome_resource_libs"> <filter type="sort_by" column="2" /> <validator type="no_options" message="No indexes are available" /> </options> </param> </inputs> <outputs> <data format="tabular" name="gmapfusion_candidates" label="${tool.name} on ${on_string}: GMAP-fusion candidates" from_work_dir="subdir/GMAP-fusion.final"/> </outputs> <tests> <test> <param name="left_input" value="GMAP/reads_1.fq.gz" /> <param name="right_input" value="GMAP/reads_2.fq.gz" /> <param name="assembled_trans" value="GMAP/transcripts.fa" /> <!-- FIX - now that we added the CTAT ref lib path as a parameter, how do we find it for testing? <param name="genome_resource_lib.fields.path" value="?????" /> --> <output name="gmapfusion_candidates" file="GMAP/fusion.reads_1_2.final" /> </test> </tests> <help> .. class:: infomark GMAP-fusion is a utility for identifying candidate fusion transcripts based on transcript sequences reconstructed via RNA-Seq de novo transcriptome assembly. Please read more here_. .. _here: https://github.com/GMAP-fusion/GMAP-fusion/wiki </help> </tool>