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1 #!/usr/bin/env python
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2 # -*- coding: utf-8 -*-
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3 import sys
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4 from Bio import SeqIO
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5 import math
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6 from parse_dis_react import *
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7 from react_norm_function import *
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8 import os
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9
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10
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11 dist_file1 = sys.argv[1] #plus library
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12 dist_file2 = sys.argv[2] #minus library
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13 seq_file = sys.argv[3] #Reference library(genome/cDNA)
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14 nt_spec = sys.argv[4] #only show reactivity for AC or ATCG
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15 flag_in = sys.argv[5] # perform 2-8% normalization (1) or not (0)
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16 threshold = sys.argv[6] #Threshold to cap the reactivities
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17 output_file = sys.argv[7]
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18
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19
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20 distri_p = parse_dist(dist_file1)
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21 distri_m = parse_dist(dist_file2)
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22 threshold = float(threshold)
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23
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24
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25 ospath = os.path.realpath(sys.argv[0])
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26 ost = ospath.split('/')
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27 syspath = ""
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28 for i in range(len(ost)-1):
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29 syspath = syspath+ost[i].strip()
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30 syspath = syspath+'/'
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31
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32 h = file(syspath+"react.txt",'w')
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33 flag_in = int(flag_in)
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34
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35 seqs = SeqIO.parse(open(seq_file),'fasta');
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36 nt_s = set()
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37 for i in range(len(nt_spec)):
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38 nt_s.add(nt_spec[i])
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39
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40 flag = 0
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41 trans = []
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42 distri_p = distri_p[1]
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43 distri_m = distri_m[1]
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44
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45 #thres = int(threshold)
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46
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47
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48 transcripts = {}
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49 for seq in seqs:
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50 n = seq.id
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51 trans.append(n)
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52 transcripts[n] = seq.seq.tostring()
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53
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54
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55 #print(distri_p)
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56
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57
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58 for i in range(0, len(trans)):
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59 h.write(trans[i])
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60 h.write('\n')
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61 for j in range(len(distri_p[trans[i]])):
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62 distri_p[trans[i]][j] = math.log((int(distri_p[trans[i]][j])+1),math.e)
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63 for j in range(len(distri_m[trans[i]])):
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64 distri_m[trans[i]][j] = math.log((int(distri_m[trans[i]][j])+1),math.e)
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65 s_p = sum(distri_p[trans[i]])
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66 s_m = sum(distri_m[trans[i]])
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67 length = len(distri_p[trans[i]])
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68 if s_p!= 0 and s_m!= 0:
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69 r = []
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70 for j in range(0, len(distri_p[trans[i]])):
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71 f_p = (float(distri_p[trans[i]][j]))/float(s_p)*length
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72 f_m = (float(distri_m[trans[i]][j]))/float(s_m)*length
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73 raw_react = f_p-f_m
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74 r.append(max(0, raw_react))
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75
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76 if s_p!= 0 and s_m!= 0:
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77 for k in range(1,(len(r)-1)):
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78 if transcripts[trans[i]][k-1] in nt_s:
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79 h.write(str(r[k]))
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80 h.write('\t')
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81 else:
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82 h.write('NA')
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83 h.write('\t')
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84 k = k+1
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85 if transcripts[trans[i]][k-1] in nt_s:
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86 h.write(str(r[k]))
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87 h.write('\n')
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88 else:
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89 h.write('NA')
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90 h.write('\n')
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91
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92
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93 h.close()
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94
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95 if flag_in:
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96 react_norm((syspath+"react.txt"),output_file, threshold)
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97 else:
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98 h_o = file(output_file, 'w')
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99 f_i = open(syspath+"react.txt")
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100 for aline in f_i.readlines():
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101 h_o.write(aline.strip())
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102 h_o.write('\n')
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103 os.system("rm -f "+syspath+"react.txt")
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