annotate alignment/phytab_prank.py @ 0:5b9a38ec4a39 draft default tip

First commit of old repositories
author osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu>
date Tue, 11 Mar 2014 12:19:13 -0700
parents
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
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osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu>
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1 import os
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osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu>
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2 import optparse
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osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu>
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3 import subprocess
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osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu>
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4 from multiprocessing import Pool
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5
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6 directory = ""
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7 results = "results.data"
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8 extension = ".fs"
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9 aligned_extension = ".afa"
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10 output_extension = ".afa.2.fas"
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11
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12
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13 def unescape(string):
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14 mapped_chars = {
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15 '>': '__gt__',
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16 '<': '__lt__',
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17 "'": '__sq__',
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18 '"': '__dq__',
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19 '[': '__ob__',
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20 ']': '__cb__',
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21 '{': '__oc__',
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22 '}': '__cc__',
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23 '@': '__at__',
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24 '\n': '__cn__',
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25 '\r': '__cr__',
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26 '\t': '__tc__',
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27 '#': '__pd__'
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28 }
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29
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30 for key, value in mapped_chars.iteritems():
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31 string = string.replace(value, key)
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32
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33 return string
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34
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35
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36 def isTabular(file):
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37 with open(file) as f:
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38 for line in f:
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39 if line[0] == '>':
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40 return False
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41 return True
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42
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43
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44 def toData(text):
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45 text = text.split('\n')
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46 result = ''
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47 for line in text:
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48 if '>' in line:
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osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu>
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49 line = '\n' + line.replace('>__XX__', "") + '\t'
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50 line = line.replace("__XX__", "\t")
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51 result += line
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osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu>
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52 return result[1:] # Index past the first newline char
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53
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54
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55 def prank(input):
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56 file_name = directory + os.sep + input
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57 popen = subprocess.Popen(['pwd'])
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58 popen.wait()
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osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu>
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59 popen = subprocess.Popen(['prank', "-d=" + file_name, "-o=" + file_name + aligned_extension, "-quiet"])
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60 popen.wait()
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61
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62 class Sequence:
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63 def __init__(self, string):
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64 lis = string.split()
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65 self.species = lis[0]
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66 self.family = lis[1]
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67 self.name = lis[2]
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68 self.header = '__XX__'.join(lis[:-1]) #prank replaces space with _ so can't join with spaces like muscle does
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69 self.sequence = lis[-1]
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70 self.string = string
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71
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72 def printFASTA(self):
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73 return '>__XX__' + self.header + '\n' + self.sequence + '\n'
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74
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75
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76 def saveMulti(tabFile):
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77 with open(tabFile) as f:
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78 for line in f:
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79 seq = Sequence(line)
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80 with open(directory + os.sep + seq.family + extension, "a") as p:
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81 p.write(seq.printFASTA())
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82
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83
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84 def saveSingle(fastaFile):
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85 with open(fastaFile) as f:
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86 for line in f:
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87 with open(directory + os.sep + "fasta" + extension, "a") as p:
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88 p.write(line)
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89
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90
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91 def main():
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92 usage = """%prog [options]
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93 options (listed below) default to 'None' if omitted
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94 """
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95 parser = optparse.OptionParser(usage=usage)
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96
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97 parser.add_option(
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98 '-d', '--directory',
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99 metavar="PATH",
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100 dest='path',
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101 default='.',
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102 help='Path to working directory.')
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103
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104 parser.add_option(
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105 '-i', '--in',
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106 dest='input',
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107 action='store',
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108 type='string',
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109 metavar="FILE",
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110 help='Name of input data.')
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111
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112 options, args = parser.parse_args()
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113
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114 global directory
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115 inputFile = unescape(options.input)
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116 directory = unescape(options.path) + os.sep + "data"
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117
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118 os.mkdir(directory)
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119
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120 if isTabular(inputFile):
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121 saveMulti(inputFile)
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122 else:
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123 saveSingle(inputFile)
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124
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125 pool = Pool()
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126 list_of_files = [file for file in os.listdir(directory) if file.lower().endswith(extension)]
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127 pool.map(prank, list_of_files)
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128 result = [file for file in os.listdir(directory) if file.lower().endswith(output_extension)]
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129 with open(directory + os.sep + results, "a") as f:
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130 for file in result:
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131 with open(directory + os.sep + file, "r") as r:
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132 f.write(toData(r.read()) + "\n")
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133
5b9a38ec4a39 First commit of old repositories
osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu>
parents:
diff changeset
134 if __name__ == '__main__':
5b9a38ec4a39 First commit of old repositories
osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu>
parents:
diff changeset
135 main()