Mercurial > repos > ucsb-phylogenetics > osiris_phylogenetics
annotate alignment/phylocatenator.xml @ 0:5b9a38ec4a39 draft default tip
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author | osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu> |
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date | Tue, 11 Mar 2014 12:19:13 -0700 |
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1 <tool id="phylocatenator" name="Phylocatenator" version="1.0.1"> |
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2 <description>Produces concatenated sequence file from phytab file of aligned sequences</description> |
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3 <command interpreter="perl"> |
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4 phylocatenator.pl $input1 $genes $species $mingene $species_file $models_file $out_file1 $partition_file $html_file > $phylocat_log |
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5 </command> |
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6 <inputs> |
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7 <!-- <display> $input1 with $genes and $species</display> --> |
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8 <param name="input1" type="data" format="tabular" label="Table containing aligned genes"/> |
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9 <param name="genes" type="integer" value="0" label="Genes" help="Minimum genes required per species. 0 retains all species. " /> |
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10 <param name="mingene" type="integer" value="35" label="Min" help="Minimum length of an aligned gene family to be included " /> |
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11 <param name="species" type="integer" value="4" label="Species" help="Minimum species per gene. 0 retains all genes." /> |
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12 <param name="species_file" type="data" format="txt" optional="true" label="Text list of species" help="Only species in the last can be retained in concatenated file" /> |
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13 <param name="models_file" type="data" format="tabular" optional="true" label="Table of: Models LUT" help="To partition data by model (protein, dna, binary, etc) according to a LUT (lookup table)" /> |
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14 <param name="outtype" type="select" label="Write as"> |
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15 <option value="R">RAxML_phylip</option> |
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16 </param> |
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17 </inputs> |
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18 <outputs> |
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19 <data format="txt" name="out_file1" metadata_source="input1" /> |
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20 <data format="txt" name="phylocat_log" label="${tool.name} on ${on_string}: Log File" /> |
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21 <data format="html" name="html_file" label="${tool.name} on ${on_string}: html Table" /> |
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22 <data format="txt" name="partition_file" label="${tool.name} on ${on_string}: Partition File" > |
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23 </data> |
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24 </outputs> |
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25 <help> |
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26 **What it does** |
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27 |
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28 This tool produces a concatenated data set for phylogenetics when not all genes are sampled for all species. |
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29 |
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30 ------ |
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31 |
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32 **Basic Example** |
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33 |
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34 The input data must be in phytab column format. Column 1 is species name, C2 is genefamily, C3 individual gene name, C4 is sequence. |
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35 Sequences of each gene family must be aligned:: |
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36 |
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37 species1 gene1 genenameA acgttagcgcgctatagc |
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38 species2 gene1 genenameB acgttag--cgctataaa |
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39 species3 gene1 genenameC acgttagcgcgctatagc |
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40 species4 gene1 genenameD acgttagcgcgctatagc |
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41 species1 gene2 genenameE --gttagtttgcta |
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42 species3 gene2 genenameF gtgttagtttgcta |
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43 |
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44 Two variables are $gene and $species. These set thresholds for |
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45 inclusion of data. $species is the minimum number of species that |
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46 contain a particular gene. $gene sets a minimum number of gene families |
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47 that a species must have to be included in the dataset. |
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48 |
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49 Running phylocatenator on the above data with 0 for genes and 0 for species yields:: |
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50 |
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51 4 32 |
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52 species1 acgttagcgcgctatagc--gttagtttgcta |
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53 species2 acgttag--cgctataaa?????????????? |
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54 species3 acgttagcgcgctatagcgtgttagtttgcta |
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55 species4 acgttagcgcgctatagc?????????????? |
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56 |
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57 **Optional Functionality** |
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58 |
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59 I. You may enter a list of species. |
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60 Species not in this list will not be written to the output file. |
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61 For example, a species list of:: |
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62 |
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63 species1 |
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64 species2 |
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65 |
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66 |
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67 Would change the above output to:: |
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68 |
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69 species1 acgttagcgcgctatagc--gttagtttgcta |
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70 species2 acgttag--cgctataaa?????????????? |
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71 |
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72 II. Table of partition models |
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73 |
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74 You may enter a table of models for each gene family/partition. Phylocatenator will then sort all the data to put all data |
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75 for the same models together. It will then create the appropriate partition file, which will specify each model in raxml. |
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76 Currently, it is only possible to partiion data into valid raxml models. |
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77 |
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78 The format is a tab-delimited file as follows:: |
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79 |
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80 gene1 WAG |
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81 gene2 JTT |
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82 gene3 DNA |
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83 gene4 WAG |
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84 |
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85 Valid models include the following:: |
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86 |
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87 BIN = binary morphological data |
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88 MULTI = multistate morphological data |
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89 DNA = DNA data |
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90 WAG = one of several protein models listed in raxml help documents |
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91 |
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92 III. Attribute |
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93 |
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94 You may enter a table with an attribute/value for each gene family/partition. Phylocatenator will then select the data based |
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95 on that value. |
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96 |
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97 The format is a tab-delimited file as follows:: |
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98 |
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99 gene1 3.1 |
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100 gene2 2.2 |
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101 gene3 0.9 |
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102 gene4 6.5 |
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103 |
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104 You can choose gene partitions based on the attribute value. |
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105 For example, if the numbers above represent rate of evolution, you could |
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106 choose to include 'slow' genes with a rate less than 2.5 |
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107 |
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108 ------ |
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109 |
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110 **Additional Information** |
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111 |
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112 http://osiris-phylogenetics.blogspot.com/2012/10/phylocatenator.html |
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113 Please direct questions or comments to ucsb_phylogenetics@lifesci.ucsb.edu or, if you can, enter them on the osiris_phylogenetics site at bitbucket.org |
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114 |
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115 ------ |
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116 |
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117 **Citation** |
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118 |
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119 This tool is part of the Osiris Phylogenetics Tool Package for Galaxy. If you make extensive use of this tool in a publication, please consider citing the following. |
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120 |
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121 Current Osiris Citation is here |
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122 |
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123 http://osiris-phylogenetics.blogspot.com/2012/10/citation.html |
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124 |
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125 First used in this paper |
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126 |
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127 Oakley, Todd H, Joanna M Wolfe, Annie R Lindgren, and Alexander K Zaharoff. 2012. Phylotranscriptomics to Bring the Understudied into the Fold: Monophyletic Ostracoda, Fossil Placement, and Pancrustacean Phylogeny. lecular Biology and Evolution. doi:10.1093/molbev/mss216. |
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128 |
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129 </help> |
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130 </tool> |