annotate alignment/phytab_mafft.py @ 0:5b9a38ec4a39 draft default tip

First commit of old repositories
author osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu>
date Tue, 11 Mar 2014 12:19:13 -0700
parents
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
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osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu>
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1 #!/usr/bin/env python
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2
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3 import os
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4 import optparse
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5 import subprocess
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6 from multiprocessing import Pool
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7
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8 directory = ""
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9 results = "results.data"
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10 extension = ".fs"
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11 aligned_extension = ".afa"
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12
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13
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14 def unescape(string):
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15 mapped_chars = {
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16 '>': '__gt__',
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17 '<': '__lt__',
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18 "'": '__sq__',
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19 '"': '__dq__',
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20 '[': '__ob__',
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21 ']': '__cb__',
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22 '{': '__oc__',
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23 '}': '__cc__',
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24 '@': '__at__',
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25 '\n': '__cn__',
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26 '\r': '__cr__',
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27 '\t': '__tc__',
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28 '#': '__pd__'
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29 }
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30
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31 for key, value in mapped_chars.iteritems():
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32 string = string.replace(value, key)
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33
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34 return string
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35
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36
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37 def isTabular(file):
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38 with open(file) as f:
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39 for line in f:
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40 if line[0] == '>':
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41 return False
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42 return True
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43
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44
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45 def toData(text):
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46 text = text.split('\n')
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47 result = ''
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48 for line in text:
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49 if '>' in line:
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50 line = '\n' + line.replace('> ', "") + '\t'
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51 line = line.replace(" ", "\t")
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52 result += line
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53 return result[1:] # Index past the first newline char
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54
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55 def toDataSingle(text):
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56 text = text.split('\n')
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57 result = ''
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58 for line in text:
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59 line = line + '\n'
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60 result += line
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61 return result[1:] # Index past the first newline char
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62
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63 def mafftauto(input):
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64 file_name = directory + os.sep + input
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65 aln = file_name + aligned_extension
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66 call = subprocess.call(['mafft', '--auto', '--out', aln, file_name])
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67
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68 def mafft1(input):
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69 file_name = directory + os.sep + input
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70 aln = file_name + aligned_extension
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71 call = subprocess.call(['mafft', '--retree', '1', '--out', aln, file_name])
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72
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73 def mafft2(input):
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74 file_name = directory + os.sep + input
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75 aln = file_name + aligned_extension
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76 call = subprocess.call(['mafft', '--retree', '2', '--out', aln, file_name])
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77
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78 def maffti(input):
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79 file_name = directory + os.sep + input
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80 aln = file_name + aligned_extension
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81 call = subprocess.call(['mafft-fftnsi', '--out', aln, file_name])
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82
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83 def maffteinsi(input):
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84 file_name = directory + os.sep + input
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85 aln = file_name + aligned_extension
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86 call = subprocess.call(['mafft-einsi', '--out', aln, file_name])
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87
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88 def mafftlinsi(input):
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89 file_name = directory + os.sep + input
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90 aln = file_name + aligned_extension
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91 call = subprocess.call(['mafft-linsi', '--out', aln, file_name])
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92
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93 def mafftginsi(input):
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94 file_name = directory + os.sep + input
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95 aln = file_name + aligned_extension
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osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu>
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96 call = subprocess.call(['mafft-ginsi', '--out', aln, file_name])
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97
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98 def mafftqinsi(input):
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99 file_name = directory + os.sep + input
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100 aln = file_name + aligned_extension
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101 call = subprocess.call(['mafft-qinsi', '--out', aln, file_name])
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102
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103
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104 class Sequence:
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105 def __init__(self, string):
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106 lis = string.split()
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107 self.species = lis[0]
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108 self.family = lis[1]
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109 self.name = lis[2]
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110 self.header = ' '.join(lis[:-1])
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111 self.sequence = lis[-1]
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112 self.string = string
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113
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114 def printFASTA(self):
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115 return '> ' + self.header + '\n' + self.sequence + '\n'
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116
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117
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118 def saveMulti(tabFile):
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119 with open(tabFile) as f:
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120 for line in f:
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121 seq = Sequence(line)
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122 with open(directory + os.sep + seq.family + extension, "a") as p:
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123 p.write(seq.printFASTA())
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124
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125
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126 def saveSingle(fastaFile):
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127 with open(fastaFile) as f:
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128 for line in f:
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129 with open(directory + os.sep + "fasta" + extension, "a") as p:
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130 p.write(line)
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131
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132
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133 def main():
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134 usage = """%prog [options]
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135 options (listed below) default to 'None' if omitted
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136 """
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137 parser = optparse.OptionParser(usage=usage)
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138
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139 parser.add_option(
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140 '-d', '--directory',
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141 metavar="PATH",
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142 dest='path',
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143 default='.',
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144 help='Path to working directory.')
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145
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146 parser.add_option(
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147 '-i', '--in',
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148 dest='input',
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149 action='store',
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150 type='string',
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151 metavar="FILE",
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152 help='Name of input data.')
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153
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154 parser.add_option(
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155 '-s', '--strat',
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156 dest='strategy',
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157 action='store',
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158 type='string',
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159 help='Alignement algorithm to use.')
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160
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161 options, args = parser.parse_args()
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162
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163 global directory
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164 inputFile = unescape(options.input)
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165 directory = unescape(options.path) + os.sep + "data"
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166 strategy = unescape(options.strategy)
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167
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168 os.mkdir(directory)
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169
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170 if isTabular(inputFile):
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171 saveMulti(inputFile)
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172 else:
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173 saveSingle(inputFile)
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174
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175 pool = Pool()
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176 list_of_files = [file for file in os.listdir(directory) if file.lower().endswith(extension)]
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177 list_of_files = sorted(list_of_files)
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178 if strategy == 'Auto':
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179 pool.map(mafftauto, list_of_files)
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180
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181 elif strategy == 'FFT-NS-1':
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182 pool.map(mafft1, list_of_files)
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183
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184 elif strategy == 'FFT-NS-2':
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185 pool.map(mafft2, list_of_files)
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186
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187 elif strategy == 'FFT-NS-i':
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188 pool.map(maffti, list_of_files)
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189
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190 elif strategy == 'E-INS-i':
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191 pool.map(maffteinsi, list_of_files)
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192
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193 elif strategy == 'L-INS-i':
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194 pool.map(mafftlinsi, list_of_files)
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195
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196 elif strategy == 'G-INS-i':
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197 pool.map(mafftginsi, list_of_files)
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198
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199 elif strategy == 'Q-INS-i':
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200 pool.map(mafftqinsi, list_of_files)
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201
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202 result = [file for file in os.listdir(directory) if file.lower().endswith(aligned_extension)]
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203 if isTabular(inputFile):
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204 with open(directory + os.sep + results, "a") as f:
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205 for file in result:
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206 with open(directory + os.sep + file, "r") as r:
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207 f.write(toData(r.read()) + "\n")
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208 else:
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209 with open(directory + os.sep + results, "a") as f:
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210 for file in result:
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211 with open(directory + os.sep + file, "r") as r:
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212 f.write(toDataSingle(r.read()) + "\n")
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213
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214 if __name__ == '__main__':
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215 main()
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216