Mercurial > repos > ucsb-phylogenetics > osiris_phylogenetics
annotate alignment/phytab_mafft.py @ 0:5b9a38ec4a39 draft default tip
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author | osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu> |
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date | Tue, 11 Mar 2014 12:19:13 -0700 |
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1 #!/usr/bin/env python |
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2 |
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3 import os |
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4 import optparse |
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5 import subprocess |
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6 from multiprocessing import Pool |
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7 |
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8 directory = "" |
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9 results = "results.data" |
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10 extension = ".fs" |
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11 aligned_extension = ".afa" |
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12 |
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13 |
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14 def unescape(string): |
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15 mapped_chars = { |
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16 '>': '__gt__', |
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17 '<': '__lt__', |
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18 "'": '__sq__', |
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19 '"': '__dq__', |
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20 '[': '__ob__', |
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21 ']': '__cb__', |
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22 '{': '__oc__', |
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23 '}': '__cc__', |
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24 '@': '__at__', |
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25 '\n': '__cn__', |
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26 '\r': '__cr__', |
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27 '\t': '__tc__', |
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28 '#': '__pd__' |
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29 } |
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30 |
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31 for key, value in mapped_chars.iteritems(): |
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32 string = string.replace(value, key) |
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33 |
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34 return string |
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35 |
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36 |
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37 def isTabular(file): |
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38 with open(file) as f: |
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39 for line in f: |
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40 if line[0] == '>': |
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41 return False |
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42 return True |
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43 |
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44 |
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45 def toData(text): |
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46 text = text.split('\n') |
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47 result = '' |
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48 for line in text: |
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49 if '>' in line: |
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50 line = '\n' + line.replace('> ', "") + '\t' |
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51 line = line.replace(" ", "\t") |
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52 result += line |
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53 return result[1:] # Index past the first newline char |
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54 |
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55 def toDataSingle(text): |
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56 text = text.split('\n') |
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57 result = '' |
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58 for line in text: |
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59 line = line + '\n' |
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60 result += line |
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61 return result[1:] # Index past the first newline char |
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62 |
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63 def mafftauto(input): |
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64 file_name = directory + os.sep + input |
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65 aln = file_name + aligned_extension |
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66 call = subprocess.call(['mafft', '--auto', '--out', aln, file_name]) |
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67 |
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68 def mafft1(input): |
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69 file_name = directory + os.sep + input |
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70 aln = file_name + aligned_extension |
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71 call = subprocess.call(['mafft', '--retree', '1', '--out', aln, file_name]) |
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72 |
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73 def mafft2(input): |
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74 file_name = directory + os.sep + input |
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75 aln = file_name + aligned_extension |
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76 call = subprocess.call(['mafft', '--retree', '2', '--out', aln, file_name]) |
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77 |
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78 def maffti(input): |
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79 file_name = directory + os.sep + input |
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80 aln = file_name + aligned_extension |
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81 call = subprocess.call(['mafft-fftnsi', '--out', aln, file_name]) |
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82 |
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83 def maffteinsi(input): |
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84 file_name = directory + os.sep + input |
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85 aln = file_name + aligned_extension |
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86 call = subprocess.call(['mafft-einsi', '--out', aln, file_name]) |
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87 |
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88 def mafftlinsi(input): |
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89 file_name = directory + os.sep + input |
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90 aln = file_name + aligned_extension |
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91 call = subprocess.call(['mafft-linsi', '--out', aln, file_name]) |
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92 |
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93 def mafftginsi(input): |
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94 file_name = directory + os.sep + input |
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95 aln = file_name + aligned_extension |
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96 call = subprocess.call(['mafft-ginsi', '--out', aln, file_name]) |
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97 |
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98 def mafftqinsi(input): |
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99 file_name = directory + os.sep + input |
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100 aln = file_name + aligned_extension |
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101 call = subprocess.call(['mafft-qinsi', '--out', aln, file_name]) |
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102 |
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103 |
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104 class Sequence: |
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105 def __init__(self, string): |
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106 lis = string.split() |
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107 self.species = lis[0] |
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108 self.family = lis[1] |
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109 self.name = lis[2] |
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110 self.header = ' '.join(lis[:-1]) |
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111 self.sequence = lis[-1] |
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112 self.string = string |
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113 |
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114 def printFASTA(self): |
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115 return '> ' + self.header + '\n' + self.sequence + '\n' |
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116 |
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117 |
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118 def saveMulti(tabFile): |
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119 with open(tabFile) as f: |
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120 for line in f: |
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121 seq = Sequence(line) |
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122 with open(directory + os.sep + seq.family + extension, "a") as p: |
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123 p.write(seq.printFASTA()) |
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124 |
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125 |
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126 def saveSingle(fastaFile): |
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127 with open(fastaFile) as f: |
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128 for line in f: |
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129 with open(directory + os.sep + "fasta" + extension, "a") as p: |
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130 p.write(line) |
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131 |
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132 |
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133 def main(): |
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134 usage = """%prog [options] |
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135 options (listed below) default to 'None' if omitted |
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136 """ |
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137 parser = optparse.OptionParser(usage=usage) |
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138 |
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139 parser.add_option( |
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140 '-d', '--directory', |
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141 metavar="PATH", |
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142 dest='path', |
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143 default='.', |
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144 help='Path to working directory.') |
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145 |
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146 parser.add_option( |
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147 '-i', '--in', |
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148 dest='input', |
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149 action='store', |
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150 type='string', |
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151 metavar="FILE", |
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152 help='Name of input data.') |
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153 |
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154 parser.add_option( |
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155 '-s', '--strat', |
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156 dest='strategy', |
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157 action='store', |
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158 type='string', |
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159 help='Alignement algorithm to use.') |
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160 |
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161 options, args = parser.parse_args() |
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162 |
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163 global directory |
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164 inputFile = unescape(options.input) |
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165 directory = unescape(options.path) + os.sep + "data" |
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166 strategy = unescape(options.strategy) |
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167 |
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168 os.mkdir(directory) |
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169 |
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170 if isTabular(inputFile): |
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171 saveMulti(inputFile) |
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172 else: |
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173 saveSingle(inputFile) |
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174 |
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175 pool = Pool() |
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176 list_of_files = [file for file in os.listdir(directory) if file.lower().endswith(extension)] |
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177 list_of_files = sorted(list_of_files) |
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178 if strategy == 'Auto': |
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179 pool.map(mafftauto, list_of_files) |
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180 |
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181 elif strategy == 'FFT-NS-1': |
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182 pool.map(mafft1, list_of_files) |
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183 |
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184 elif strategy == 'FFT-NS-2': |
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185 pool.map(mafft2, list_of_files) |
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186 |
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187 elif strategy == 'FFT-NS-i': |
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188 pool.map(maffti, list_of_files) |
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189 |
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190 elif strategy == 'E-INS-i': |
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191 pool.map(maffteinsi, list_of_files) |
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192 |
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193 elif strategy == 'L-INS-i': |
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194 pool.map(mafftlinsi, list_of_files) |
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195 |
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196 elif strategy == 'G-INS-i': |
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197 pool.map(mafftginsi, list_of_files) |
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198 |
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199 elif strategy == 'Q-INS-i': |
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200 pool.map(mafftqinsi, list_of_files) |
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201 |
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202 result = [file for file in os.listdir(directory) if file.lower().endswith(aligned_extension)] |
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203 if isTabular(inputFile): |
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204 with open(directory + os.sep + results, "a") as f: |
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205 for file in result: |
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206 with open(directory + os.sep + file, "r") as r: |
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207 f.write(toData(r.read()) + "\n") |
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208 else: |
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209 with open(directory + os.sep + results, "a") as f: |
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210 for file in result: |
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211 with open(directory + os.sep + file, "r") as r: |
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212 f.write(toDataSingle(r.read()) + "\n") |
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213 |
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214 if __name__ == '__main__': |
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215 main() |
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216 |