Mercurial > repos > ucsb-phylogenetics > osiris_phylogenetics
diff alignment/gblocks.xml @ 0:5b9a38ec4a39 draft default tip
First commit of old repositories
author | osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu> |
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date | Tue, 11 Mar 2014 12:19:13 -0700 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/alignment/gblocks.xml Tue Mar 11 12:19:13 2014 -0700 @@ -0,0 +1,93 @@ +<tool id="gblocks" name="Gblocks" version="1.0"> + <description>Convert Aligned FASTA to phylip Extended</description> + <command interpreter="perl"> + gblocks.pl $input $datatype $gaps $Block $out_file $html_file > $screen + </command> + <inputs> + <param name="input" type="data" format="fasta" label="Input Fasta File" help="Aligned fasta" /> + <param name="datatype" type="select" label="Type of Sequence"> + <option value="-t=d">DNA</option> + <option value="-t=p">Protein</option> + <option value="-t=c">Codons</option> + </param> + <param name="Block" type="integer" value="10" label="Minimum block size" help='10 is default. Smaller values less stringent.'/> + <param name="gaps" type="select" label="Gap Allowance"> + <option value="-b5=n">None</option> + <option value="-b5=h">Half</option> + <option value="-b5=a">All</option> + </param> + </inputs> + <outputs> + <data format="txt" name="screen" label="${tool.name} on ${on_string}: screen Log" /> + <data format="html" name="html_file" label="${tool.name} on ${on_string}: html File"/> + <data format="txt" name="out_file" label="${tool.name} on ${on_string}: Output File"/> + </outputs> + <help> +**What it does** + +About GBlocks + +Version 0.91b, January 2002 + +Copyright Jose Castresana + +Gblocks is a computer program written in ANSI C language that eliminates poorly aligned +positions and divergent regions of an alignment of DNA or protein sequences. These +positions may not be homologous or may have been saturated by multiple substitutions and it +is convenient to eliminate them prior to phylogenetic analysis. Gblocks selects blocks in a +similar way as it is usually done by hand but following a reproducible set of conditions. +The selected blocks must fulfill certain requirements with respect to the lack of large +segments of contiguous nonconserved positions, lack of gap positions and high conservation +of flanking positions, making the final alignment more suitable for phylogenetic analysis. +Gblocks outputs several files to visualize the selected blocks. The use of a program such +as Gblocks reduces the necessity of manually editing multiple alignments, makes the +automation of phylogenetic analysis of large data sets feasible and, finally, facilitates +the reproduction of the alignments and subsequent phylogenetic analysis by other +researchers. Gblocks is very fast in processing alignments and it is therefore highly +suitable for large-scale phylogenetic analyses. + +Several parameters can be modified to make the selection of blocks more or less stringent. +In general, a relaxed selection of blocks is better for short alignments, whereas a +stringent selection is more adequate for longer ones. Be aware that the default options of +Gblocks are stringent. + +------ + +**Inputs** + +FASTA + +------ + +**Outputs** + +Phylip extended + +------- + +**Additional Information** + +Please direct questions or comments to ucsb_phylogenetics@lifesci.ucsb.edu or, if you can, enter them on the osiris_phylogenetics site at +bitbucket.org + +------- + +**Citations** + +This tool is part of the Osiris Phylogenetics Tool Package for Galaxy. If you make extensive use of this tool in a publication, please consider +citing the following. + +Current Osiris Citation is here + +http://osiris-phylogenetics.blogspot.com/2012/10/citation.html + +Additional Citations for this tool + +Talavera, G., and Castresana, J. (2007). Improvement of phylogenies after removing +divergent and ambiguously aligned blocks from protein sequence alignments. Systematic +Biology 56, 564-577. + +Castresana, J. (2000). Selection of conserved blocks from multiple alignments for their +use in phylogenetic analysis. Molecular Biology and Evolution 17, 540-552. + </help> +</tool>