Mercurial > repos > ucsb-phylogenetics > osiris_phylogenetics
diff alignment/phytab_ssr.py @ 0:5b9a38ec4a39 draft default tip
First commit of old repositories
author | osiris_phylogenetics <ucsb_phylogenetics@lifesci.ucsb.edu> |
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date | Tue, 11 Mar 2014 12:19:13 -0700 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/alignment/phytab_ssr.py Tue Mar 11 12:19:13 2014 -0700 @@ -0,0 +1,170 @@ +#!/usr/bin/env python + +import os +import optparse +import subprocess +from multiprocessing import Pool +from functools import partial + +directory = "" +results = "results.data" +extension = ".fs" +slim_extension = ".slim" +jarpath='/home/galaxy/pkgs/SSR/SimilarSequenceRemover.jar' + +def unescape(string): + mapped_chars = { + '>': '__gt__', + '<': '__lt__', + "'": '__sq__', + '"': '__dq__', + '[': '__ob__', + ']': '__cb__', + '{': '__oc__', + '}': '__cc__', + '@': '__at__', + '\n': '__cn__', + '\r': '__cr__', + '\t': '__tc__', + '#': '__pd__' + } + + for key, value in mapped_chars.iteritems(): + string = string.replace(value, key) + + return string + + +def isTabular(file): + with open(file) as f: + for line in f: + if line[0] == '>': + return False + return True + + +def toData(text): + text = text.split('\n') + result = '' + for line in text: + if '>' in line: + line = '\n' + line.replace('> ', "") + '\t' + line = line.replace(" ", "\t") + result += line + return result[1:] # Index past the first newline char + +def toDataSingle(text): + text = text.split('\n') + result = '' + for line in text: + line = line + '\n' + result += line + return result[1:] # Index past the first newline char + +def runSimilarSequenceRemover(input): + input_name = directory + os.sep + input + outfasta = input_name + slim_extension + call = subprocess.call(['java','-jar', jarpath, '-i', input_name, '-o', outfasta, 's=' + percentage, '-h', alignallornot ]) + +class Sequence: + def __init__(self, string): + lis = string.split() + self.species = lis[0] + self.family = lis[1] + self.name = lis[2] + self.header = ' '.join(lis[:-1]) + self.sequence = lis[-1] + self.string = string + + def printFASTA(self): + return '> ' + self.header + '\n' + self.sequence + '\n' + + +def saveMulti(tabFile): + with open(tabFile) as f: + for line in f: + seq = Sequence(line) + with open(directory + os.sep + seq.family + extension, "a") as p: + p.write(seq.printFASTA()) + + +def saveSingle(fastaFile): + with open(fastaFile) as f: + for line in f: + with open(directory + os.sep + "fasta" + extension, "a") as p: + p.write(line) + + +def main(): + usage = """%prog [options] +options (listed below) default to 'None' if omitted + """ + parser = optparse.OptionParser(usage=usage) + + parser.add_option( + '-d', '--directory', + metavar="PATH", + dest='path', + default='.', + help='Path to working directory.') + + parser.add_option( + '-i', '--in', + dest='input', + action='store', + type='string', + metavar="FILE", + help='Name of input data.') + + parser.add_option( + '-a', '--alignall', + dest='alignall', + action='store', + type='string', help='t or f. t only aligns first 100 sites instead of entire seq') + + parser.add_option( + '-s', '--similarity', + dest='similarity', + action='store', + type='float', + help='Percentage similarity as cutoff (eg 0.99).') + + options, args = parser.parse_args() + + global directory + global percentage + global alignallornot + inputFile = unescape(options.input) + directory = unescape(options.path) + os.sep + "data" + percentage = str(options.similarity) + alignallornot = options.alignall + + os.mkdir(directory) + + if isTabular(inputFile): + saveMulti(inputFile) + else: + saveSingle(inputFile) + + pool = Pool() + list_of_files = [file for file in os.listdir(directory) if file.lower().endswith(extension)] + list_of_files = sorted(list_of_files) + + pool.map(runSimilarSequenceRemover, list_of_files) + + result = [file for file in os.listdir(directory) if file.lower().endswith(slim_extension)] + if isTabular(inputFile): + with open(directory + os.sep + results, "a") as f: + for file in result: + with open(directory + os.sep + file, "r") as r: + f.write(toData(r.read()) + "\n") + else: + with open(directory + os.sep + results, "a") as f: + for file in result: + with open(directory + os.sep + file, "r") as r: + f.write(r.read() +'\n') + +if __name__ == '__main__': + main() + +