Mercurial > repos > vimalkumarvelayudhan > riboseqr_wrapper
view README.rst @ 0:c34c364ce75d
First commit
| author | Vimalkumar Velayudhan <vimal@biotechcoder.com> | 
|---|---|
| date | Tue, 21 Jul 2015 14:48:39 +0100 | 
| parents | |
| children | d7ce95ccf54f 423ad61697c4 | 
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riboseqr_wrapper ---------------- `riboSeqR <http://bioconductor.org/packages/3.0/bioc/html/riboSeqR.html>`_ integration for `Galaxy <http://galaxyproject.org/>`_ and `RiboGalaxy <http://ribogalaxy.ucc.ie/>`_. Included tools .............. In the order they are run 1. Prepare riboSeqR Input - Prepare riboSeqR format input files from SAM format alignment files. The SAM format files should be obtained by aligning Ribo-Seq and RNA-Seq data to the transcriptome. (RNA-Seq data is optional but required for Differential translation analysis). 2. Triplet Periodicity - Plot triplet periodicity for different read lengths. 3. Metagene Analysis 4. Plot Ribosome Profile [OR] Differential Translation Analysis - Get Ribo and RNA-Seq counts with riboSeqR. Perform differential translation analysis with baySeq. Dependencies ............ Tested on Ubuntu Linux 14.04 LTS, 64-bit. Dependencies should install automatically on Linux x86_64. **R** ``3.1.2`` **riboSeqR** ``1.0.5`` **baySeq** ``2.0.50`` **rpy2** ``2.3.10``
