Mercurial > repos > vipints > deseq_hts
diff deseq-hts_1.0/README @ 9:e27b4f7811c2 draft
Updated DESeq version 1.12
author | vipints <vipin@cbio.mskcc.org> |
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date | Tue, 08 Oct 2013 08:09:28 -0400 |
parents | 8ab01cc29c4b |
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--- a/deseq-hts_1.0/README Wed Jun 27 15:38:39 2012 -0400 +++ b/deseq-hts_1.0/README Tue Oct 08 08:09:28 2013 -0400 @@ -1,48 +1,52 @@ ---------------------------------------------------- -DESeq-hts: A Galaxy wrapper for DESeq version 1.6.1 ---------------------------------------------------- +---------------------------------------------------- +DESeq: A Galaxy wrapper for DESeq version 1.12.1 +---------------------------------------------------- Description: +------------ DESeq can be used as a web service embedded in a Galaxy instance. We call it as DESeq-hts. -Requirements: +Requirements: +------------- MATLAB/OCTAVE and Python :- Preprocessing of sequencing reads and GFF files - R, Bio-conductor package :- Required for DESEQ + R, Bio-conductor package :- Required for DESeq SCIPY, NUMPY :- for python - SAMTOOLS :- Read processing + SAMTOOLS :- Sequencing read processing Contents: - [src] +--------- + ./src All relevant scripts for DESeq-hts are located in the subdirectory - src. src/deseq.sh is the main script to start DESeq-hts. The - preprocessing of BAM and GFF file start before the R DESEQ script. + src. src/deseq-hts.sh is the main script to start DESeq-hts. The + preprocessing of BAM and GFF file start before the R DESeq script. Please follow the shell script to understand the details. - [galaxy] + ./galaxy Galaxy tool configuration file can be found galaxy folder. Please make necessary editing for .xml file and remaining .sh files and perform few tests. - [setup_deseq-hts.sh] + ./setup_deseq-hts.sh Setup script for DESeq-hts. - [mex] + ./mex matlab executable files. - [bin] + ./bin Contains deseq_config.sh file which is used for the configuration of DESeq-hts. According to your platform, the default file will be changed. - [test_data] - This subsirectory contains all data for running a functional test in + ./test_data + This sub-directory contains all data for running a functional test in Galaxy framework. You may need to move these test files into the test-data directory. - [tools] + ./tools A python based GFF parsing program. Also contains small utils programs. Getting started: +---------------- Check for all requirements first, then a) Run ./setup_deseq-hts.sh and setup paths and configuration options for DESeq-hts. @@ -57,6 +61,7 @@ c) Edit the Galaxy tool configuration file to adjust the path if necessary. Licenses: +--------- If **DESeq** is used to obtain results for scientific publications it should be cited as [1]. This wrapper program (DESeq-hts) is free software; you can redistribute it and/or modify it @@ -65,11 +70,21 @@ Written (W) 2009-2012 Jonas Behr, Regina Bohnert, Andre Kahles, Gunnar Raetsch, Vipin T. Sreedharan Copyright (C) 2009-2012 Friedrich Miescher Laboratory of the Max Planck Society, Tubingen, Germany and - 2012 cBio Memorial Sloan Kettering Cancer Center, New York City, USA. + 2013 cBio Memorial Sloan Kettering Cancer Center, New York City, USA. References: +----------- [1] Anders, S and Huber, W (2010): `Differential expression analysis for sequence count data`. +Contributions: +-------------- + 15 Aug. 2013 + Philippe Moncuquet + Bioinformatics Analyst, Bioinformatics Core, CSIRO Mathematics, Informatics and Statistics + + Extended the DESeq result. + Contact: - vipin@cbio.mskcc.org +-------- + support [at] oqtans.org