Mercurial > repos > vipints > deseq_hts
diff deseq-hts_1.0/galaxy/deseq.xml @ 9:e27b4f7811c2 draft
Updated DESeq version 1.12
author | vipints <vipin@cbio.mskcc.org> |
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date | Tue, 08 Oct 2013 08:09:28 -0400 |
parents | 8ab01cc29c4b |
children |
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--- a/deseq-hts_1.0/galaxy/deseq.xml Wed Jun 27 15:38:39 2012 -0400 +++ b/deseq-hts_1.0/galaxy/deseq.xml Tue Oct 08 08:09:28 2013 -0400 @@ -1,7 +1,10 @@ -<tool id="deseq-hts" name="DESeq" version="1.6.1"> - <description>Determines differentially expressed transcripts from read alignments</description> - <command> -deseq-hts/src/deseq-hts.sh $anno_input_selected $deseq_out $deseq_out.extra_files_path/gene_map.mat +<tool id="deseq-hts" name="DESeq" version="1.12.1"> + <description> Determines differentially expressed transcripts from read alignments</description> + <requirements> + <requirement type="package" version="0.1">oqtans</requirement> + </requirements> + <command interpreter="bash"> +./../src/deseq-hts.sh $anno_input_selected $deseq_out $deseq_out.extra_files_path/gene_map.mat #for $i in $replicate_groups #for $j in $i.replicates $j.bam_alignment:#slurp @@ -19,8 +22,8 @@ </inputs> <outputs> - <data format="txt" name="deseq_out" label="DESeq result"/> - <data format="txt" name="Log_File" label="DESeq log file"/> + <data format="txt" name="deseq_out" label="${tool.name} on ${on_string}: Differential Expression"/> + <data format="txt" name="Log_File" label="${tool.name} on ${on_string}: log"/> </outputs> <tests> @@ -41,19 +44,15 @@ **What it does** -`DESeq` is a tool for differential expression testing of RNA-Seq data. - +DESeq_ is a tool for differential expression testing of RNA-Seq data. -**Inputs** - -`DESeq` requires three input files to run: +.. _DESeq: http://bioconductor.org/packages/release/bioc/html/DESeq.html -1. Annotation file in GFF3, containing the necessary information about the transcripts that are to be quantified. -2. The BAM alignment files grouped into replicate groups, each containing several replicates. BAM files store the read alignments in a compressed format. They can be generated using the `SAM-to-BAM` tool in the NGS: SAM Tools section. (The script will also work with only two groups containing only a single replicate each. However, this analysis has less statistical power and is therefor not recommended.) +`DESeq` requires: -**Output** +Genome annotation file in GFF3, containing the necessary information about the transcripts that are to be quantified. -`DESeq` generates a text file containing the gene name and the p-value. +The BAM alignment files grouped into replicate groups, each containing several replicates. BAM files store the read alignments, The program will also work with only two groups containing only a single replicate each. However, this analysis has less statistical power and is therefore not recommended! ------ @@ -100,7 +99,7 @@ ------ -DESeq-hts Wrapper Version 0.3 (Feb 2012) +DESeq-hts Wrapper Version 0.5 (Aug 2013) </help> </tool>