Mercurial > repos > vipints > fml_gff3togtf
diff bed_to_gff.xml @ 10:c42c69aa81f8
fixed manually the upload of version 2.1.0 - deleted accidentally added files to the repo
author | vipints <vipin@cbio.mskcc.org> |
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date | Thu, 23 Apr 2015 18:01:45 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/bed_to_gff.xml Thu Apr 23 18:01:45 2015 -0400 @@ -0,0 +1,95 @@ +<tool id="fml_bed2gff" name="BED-to-GFF" version="2.1.0"> + <description>converter</description> + <command interpreter="python">bed_to_gff.py $inf_bed > $gff_format + </command> + <inputs> + <param format="bed" name="inf_bed" type="data" label="Convert this query" help="Provide genome annotation in 12 column BED format."/> + </inputs> + <outputs> + <data format="gff" name="gff_format" label="${tool.name} on ${on_string}: Converted" /> + </outputs> + <tests> + <test> + <param name="inf_bed" value="CCDS30770.bed" /> + <output name="gff_format" file="CCDS30770.gff" /> + </test> + </tests> + <help> + +**What it does** + +This tool converts data from a 12 column UCSC wiggle BED format to GFF3 (scroll down for format description). + +-------- + +**Example** + +- The following data in UCSC Wiggle BED format:: + + chr1 11873 14409 uc001aaa.3 0 + 11873 11873 0 3 354,109,1189, 0,739,1347, + +- Will be converted to GFF3:: + + ##gff-version 3 + chr1 bed2gff gene 11874 14409 0 + . ID=Gene:uc001aaa.3;Name=Gene:uc001aaa.3 + chr1 bed2gff transcript 11874 14409 0 + . ID=uc001aaa.3;Name=uc001aaa.3;Parent=Gene:uc001aaa.3 + chr1 bed2gff exon 11874 12227 0 + . Parent=uc001aaa.3 + chr1 bed2gff exon 12613 12721 0 + . Parent=uc001aaa.3 + chr1 bed2gff exon 13221 14409 0 + . Parent=uc001aaa.3 + +-------- + +**Reference** + +**BED-to-GFF** is part of oqtans package and cited as [1]_. + +.. [1] Sreedharan VT, Schultheiss SJ, Jean G et.al., Oqtans: the RNA-seq workbench in the cloud for complete and reproducible quantitative transcriptome analysis. Bioinformatics (2014). `10.1093/bioinformatics/btt731`_ + +.. _10.1093/bioinformatics/btt731: http://goo.gl/I75poH + +-------- + +**About file formats** + +**BED format** Browser Extensible Data format was designed at UCSC for displaying data tracks in the Genome Browser. It has three required fields and several additional optional ones: + +The first three BED fields (required) are:: + + 1. chrom - The name of the chromosome (e.g. chr1, chrY_random). + 2. chromStart - The starting position in the chromosome. (The first base in a chromosome is numbered 0.) + 3. chromEnd - The ending position in the chromosome, plus 1 (i.e., a half-open interval). + +The additional BED fields (optional) are:: + + 4. name - The name of the BED line. + 5. score - A score between 0 and 1000. + 6. strand - Defines the strand - either '+' or '-'. + 7. thickStart - The starting position where the feature is drawn thickly at the Genome Browser. + 8. thickEnd - The ending position where the feature is drawn thickly at the Genome Browser. + 9. reserved - This should always be set to zero. + 10. blockCount - The number of blocks (exons) in the BED line. + 11. blockSizes - A comma-separated list of the block sizes. The number of items in this list should correspond to blockCount. + 12. blockStarts - A comma-separated list of block starts. All of the blockStart positions should be calculated relative to chromStart. The number of items in this list should correspond to blockCount. + +**GFF format** General Feature Format is a format for describing genes and other features associated with DNA, RNA and Protein sequences. GFF lines have nine tab-separated fields:: + + 1. seqid - Must be a chromosome or scaffold or contig. + 2. source - The program that generated this feature. + 3. type - The name of this type of feature. Some examples of standard feature types are "gene", "CDS", "protein", "mRNA", and "exon". + 4. start - The starting position of the feature in the sequence. The first base is numbered 1. + 5. stop - The ending position of the feature (inclusive). + 6. score - A score between 0 and 1000. If there is no score value, enter ".". + 7. strand - Valid entries include '+', '-', or '.' (for don't know/care). + 8. phase - If the feature is a coding exon, frame should be a number between 0-2 that represents the reading frame of the first base. If the feature is not a coding exon, the value should be '.'. + 9. attributes - All lines with the same group are linked together into a single item. + +-------- + +**Copyright** + +BED-to-GFF Wrapper Version 0.6 (Apr 2015) + +2009-2015 Max Planck Society, University of Tübingen & Memorial Sloan Kettering Cancer Center + + </help> +</tool>