Mercurial > repos > vipints > fml_gff3togtf
diff gtf_to_gff.xml @ 10:c42c69aa81f8
fixed manually the upload of version 2.1.0 - deleted accidentally added files to the repo
author | vipints <vipin@cbio.mskcc.org> |
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date | Thu, 23 Apr 2015 18:01:45 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/gtf_to_gff.xml Thu Apr 23 18:01:45 2015 -0400 @@ -0,0 +1,92 @@ +<tool id="fml_gtf2gff" name="GTF-to-GFF" version="2.1.0"> + <description>converter</description> + <command interpreter="python">gtf_to_gff.py $inf_gtf > $gff3_format + </command> + <inputs> + <param format="gtf" name="inf_gtf" type="data" label="Convert this query" help="Provide genome annotation file in GTF."/> + </inputs> + <outputs> + <data format="gff" name="gff3_format" label="${tool.name} on ${on_string}: Converted" /> + </outputs> + <tests> + <test> + <param name="inf_gtf" value="aceview_hs_37.gtf" /> + <output name="gff3_format" file="aceview_hs_37.gff3" /> + </test> + </tests> + <help> + +**What it does** + +This tool converts data from GTF to a valid GFF file (scroll down for format description). + +-------- + +**Example** + +- The following data in GTF:: + + 17 protein_coding exon 7255208 7258258 . + . gene_id "ENSG00000213859"; transcript_id "ENST00000333751"; exon_number "1"; gene_name "KCTD11"; transcript_name "KCTD11-001"; + 17 protein_coding CDS 7256262 7256957 . + 0 gene_id "ENSG00000213859"; transcript_id "ENST00000333751"; exon_number "1"; gene_name "KCTD11"; transcript_name "KCTD11-001"; protein_id "ENSP00000328352"; + 17 protein_coding start_codon 7256262 7256264 . + 0 gene_id "ENSG00000213859"; transcript_id "ENST00000333751"; exon_number "1"; gene_name "KCTD11"; transcript_name "KCTD11-001"; + 17 protein_coding stop_codon 7256958 7256960 . + 0 gene_id "ENSG00000213859"; transcript_id "ENST00000333751"; exon_number "1"; gene_name "KCTD11"; transcript_name "KCTD11-001"; + +- Will be converted to GFF3:: + + ##gff-version 3 + 17 protein_coding gene 7255208 7258258 . + . ID=ENSG00000213859;Name=KCTD11 + 17 protein_coding mRNA 7255208 7258258 . + . ID=ENST00000333751;Name=KCTD11-001;Parent=ENSG00000213859 + 17 protein_coding protein 7256262 7256960 . + . ID=ENSP00000328352;Name=KCTD11-001;Parent=ENST00000333751 + 17 protein_coding five_prime_UTR 7255208 7256261 . + . Parent=ENST00000333751 + 17 protein_coding CDS 7256262 7256960 . + 0 Name=CDS:KCTD11;Parent=ENST00000333751,ENSP00000328352 + 17 protein_coding three_prime_UTR 7256961 7258258 . + . Parent=ENST00000333751 + 17 protein_coding exon 7255208 7258258 . + . Parent=ENST00000333751 + +-------- + +**Reference** + +**GTF-to-GFF** is part of oqtans package and cited as [1]_. + +.. [1] Sreedharan VT, Schultheiss SJ, Jean G et.al., Oqtans: the RNA-seq workbench in the cloud for complete and reproducible quantitative transcriptome analysis. Bioinformatics (2014). `10.1093/bioinformatics/btt731`_ + +.. _10.1093/bioinformatics/btt731: http://goo.gl/I75poH + +------ + +**About formats** + +**GTF format** Gene Transfer Format, it borrows from GFF, but has additional structure that warrants a separate definition and format name. GTF lines have nine tab-seaparated fields:: + + 1. seqname - The name of the sequence. + 2. source - This indicating where the annotation came from. + 3. feature - The name of the feature types. The following feature types are required: 'CDS', 'start_codon' and 'stop_codon' + 4. start - The starting position of the feature in the sequence. The first base is numbered 1. + 5. end - The ending position of the feature (inclusive). + 6. score - The score field indicates a degree of confidence in the feature's existence and coordinates. + 7. strand - Valid entries include '+', '-', or '.' + 8. frame - If the feature is a coding exon, frame should be a number between 0-2 that represents the reading frame of the first base. + 9. attributes - These attributes are designed for handling multiple transcripts from the same genomic region. + +**GFF format** General Feature Format is a format for describing genes and other features associated with DNA, RNA and Protein sequences. GFF lines have nine tab-separated fields:: + + 1. seqid - Must be a chromosome or scaffold. + 2. source - The program that generated this feature. + 3. type - The name of this type of feature. Some examples of standard feature types are "gene", "CDS", "protein", "mRNA", and "exon". + 4. start - The starting position of the feature in the sequence. The first base is numbered 1. + 5. stop - The ending position of the feature (inclusive). + 6. score - A score between 0 and 1000. If there is no score value, enter ".". + 7. strand - Valid entries include '+', '-', or '.' (for don't know/care). + 8. phase - If the feature is a coding exon, frame should be a number between 0-2 that represents the reading frame of the first base. If the feature is not a coding exon, the value should be '.'. + 9. attributes - All lines with the same group are linked together into a single item. + +-------- + +**Copyright** + +GTF-to-GFF Wrapper Version 0.6 (Apr 2015) + +2009-2015 Max Planck Society, University of Tübingen & Memorial Sloan Kettering Cancer Center + + </help> +</tool>