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view gff_fmap.xml @ 5:6e589f267c14
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author | devteam |
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date | Tue, 04 Nov 2014 12:15:19 -0500 |
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<tool id="fml_gfffmap" name="GFF-map" version="2.0.0"> <description>features</description> <command interpreter="python"> gff_fmap.py $gff_input > $idmapping </command> <inputs> <param format="gff3,gff" name="gff_input" type="data" label="Query file" help="Provide genome annotation file in GFF."/> </inputs> <outputs> <data format="txt" name="idmapping" label="${tool.name} on ${on_string}: parent child id map"/> </outputs> <tests> <test> <param name="gff_input" value="Feature_ID_mapping_W.gff3" /> <output name="idmapping" file="Feature_ID_mapping_W.txt" /> </test> <test> <param name="gff_input" value="Aly_JGI.gff3" /> <output name="idmapping" file="Feature_ID_mapping_R.txt" /> </test> </tests> <help> **What it does** GFF-map provides the features (gene, mRNA, UTR's, exon, CDS etc) relationship based on their identifier mapping in a given GFF file. -------- **Example** - The features ID mapping in the following data in GFF3:: ##gff-version 3 17 protein_coding gene 7255208 7258258 . + . ID=ENSG00000213859;Name=KCTD11 17 protein_coding mRNA 7255208 7258258 . + . ID=ENST00000333751;Name=KCTD11-001;Parent=ENSG00000213859 17 protein_coding protein 7256262 7256960 . + 0 ID=ENSP00000328352;Name=ENSP00000328352 17 protein_coding five_prime_UTR 7255208 7256261 . + . Parent=ENST00000333751 17 protein_coding CDS 7256262 7256960 . + 0 Name=CDS:KCTD11;Parent=ENST00000333751,ENSP00000328352 17 protein_coding three_prime_UTR 7256961 7258258 . + . Parent=ENST00000333751 17 protein_coding exon 7255208 7258258 . + . Parent=ENST00000333751 - Will be displayed as:: +-----------------------+---------------------------------+ | Parent feature type | Associated child feature type(s)| +-----------------------+---------------------------------+ | protein_coding gene | protein_coding mRNA | +-----------------------+---------------------------------+ | protein_coding protein| protein_coding CDS | +-----------------------+---------------------------------+ | protein_coding mRNA | protein_coding CDS | | | protein_coding exon | | | protein_coding five_prime_UTR | | | protein_coding three_prime_UTR | +-----------------------+---------------------------------+ -------- **About formats** **GFF3 format** General Feature Format is a format for describing genes and other features associated with DNA, RNA and Protein sequences. GFF3 lines have nine tab-separated fields:: 1. seqid - Must be a chromosome or scaffold. 2. source - The program that generated this feature. 3. type - The name of this type of feature. Some examples of standard feature types are "gene", "CDS", "protein", "mRNA", and "exon". 4. start - The starting position of the feature in the sequence. The first base is numbered 1. 5. stop - The ending position of the feature (inclusive). 6. score - A score between 0 and 1000. If there is no score value, enter ".". 7. strand - Valid entries include '+', '-', or '.' (for don't know/care). 8. phase - If the feature is a coding exon, frame should be a number between 0-2 that represents the reading frame of the first base. If the feature is not a coding exon, the value should be '.'. 9. attributes - All lines with the same group are linked together into a single item. -------- **Copyright** 2009-2014 Max Planck Society, University of Tübingen & Memorial Sloan Kettering Cancer Center Sreedharan VT, Schultheiss SJ, Jean G, Kahles A, Bohnert R, Drewe P, Mudrakarta P, Görnitz N, Zeller G, Rätsch G. Oqtans: the RNA-seq workbench in the cloud for complete and reproducible quantitative transcriptome analysis. Bioinformatics 10.1093/bioinformatics/btt731 (2014) </help> </tool>