Mercurial > repos > vipints > fml_gff3togtf
view gff_to_gtf.xml @ 7:ee541c1852da
Uploaded version 2.1.0
author | vipints |
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date | Thu, 23 Apr 2015 17:43:11 -0400 |
parents | 6e589f267c14 |
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<tool id="fml_gff2gtf" name="GFF-to-GTF" version="2.0.0"> <description>converter</description> <command interpreter="python">gff_to_gtf.py $inf_gff3 > $gtf_format </command> <inputs> <param format="gff3,gff" name="inf_gff3" type="data" label="Convert this query" help="Provide genome annotation file in GFF or GFF3."/> </inputs> <outputs> <data format="gtf" name="gtf_format" label="${tool.name} on ${on_string}: Converted" /> </outputs> <tests> <test> <param name="inf_gff3" value="AceView_ncbi_37.gff3" /> <output name="gtf_format" file="AceView_gff3_to_gtf.gtf" /> </test> <test> <param name="inf_gff3" value="ENSEMBL_mm9.gff3" /> <output name="gtf_format" file="ENSEMBL_mm9_gff3_to_gtf.gtf" /> </test> </tests> <help> **What it does** This tool converts data from GFF3 to GTF file format (scroll down for format description). -------- **Example** - The following data in GFF3 format:: ##gff-version 3 17 protein_coding gene 7255208 7258258 . + . ID=ENSG00000213859;Name=KCTD11 17 protein_coding mRNA 7255208 7258258 . + . ID=ENST00000333751;Name=KCTD11-001;Parent=ENSG00000213859 17 protein_coding protein 7256262 7256960 . + . ID=ENSP00000328352;Name=KCTD11-001;Parent=ENST00000333751 17 protein_coding five_prime_UTR 7255208 7256261 . + . Parent=ENST00000333751 17 protein_coding CDS 7256262 7256960 . + 0 Name=CDS:KCTD11;Parent=ENST00000333751,ENSP00000328352 17 protein_coding three_prime_UTR 7256961 7258258 . + . Parent=ENST00000333751 17 protein_coding exon 7255208 7258258 . + . Parent=ENST00000333751 - Will be converted to GTF format:: 17 protein_coding exon 7255208 7258258 . + . gene_id "ENSG00000213859"; transcript_id "ENST00000333751"; exon_number "1"; gene_name "KCTD11"; transcript_name "KCTD11-001"; 17 protein_coding CDS 7256262 7256957 . + 0 gene_id "ENSG00000213859"; transcript_id "ENST00000333751"; exon_number "1"; gene_name "KCTD11"; transcript_name "KCTD11-001"; protein_id "ENSP00000328352"; 17 protein_coding start_codon 7256262 7256264 . + 0 gene_id "ENSG00000213859"; transcript_id "ENST00000333751"; exon_number "1"; gene_name "KCTD11"; transcript_name "KCTD11-001"; 17 protein_coding stop_codon 7256958 7256960 . + 0 gene_id "ENSG00000213859"; transcript_id "ENST00000333751"; exon_number "1"; gene_name "KCTD11"; transcript_name "KCTD11-001"; -------- **About formats** **GFF3 format** General Feature Format is a format for describing genes and other features associated with DNA, RNA and Protein sequences. GFF3 lines have nine tab-separated fields:: 1. seqid - Must be a chromosome or scaffold. 2. source - The program that generated this feature. 3. type - The name of this type of feature. Some examples of standard feature types are "gene", "CDS", "protein", "mRNA", and "exon". 4. start - The starting position of the feature in the sequence. The first base is numbered 1. 5. stop - The ending position of the feature (inclusive). 6. score - A score between 0 and 1000. If there is no score value, enter ".". 7. strand - Valid entries include '+', '-', or '.' (for don't know/care). 8. phase - If the feature is a coding exon, frame should be a number between 0-2 that represents the reading frame of the first base. If the feature is not a coding exon, the value should be '.'. 9. attributes - All lines with the same group are linked together into a single item. **GTF format** Gene Transfer Format, it borrows from GFF, but has additional structure that warrants a separate definition and format name. GTF lines have nine tab-seaparated fields:: 1. seqname - The name of the sequence. 2. source - This indicating where the annotation came from. 3. feature - The name of the feature types. The following feature types are required: 'CDS', 'start_codon' and 'stop_codon' 4. start - The starting position of the feature in the sequence. The first base is numbered 1. 5. end - The ending position of the feature (inclusive). 6. score - The score field indicates a degree of confidence in the feature's existence and coordinates. 7. strand - Valid entries include '+', '-', or '.' 8. frame - If the feature is a coding exon, frame should be a number between 0-2 that represents the reading frame of the first base. 9. attributes - These attributes are designed for handling multiple transcripts from the same genomic region. -------- **Copyright** 2009-2014 Max Planck Society, University of Tübingen & Memorial Sloan Kettering Cancer Center Sreedharan VT, Schultheiss SJ, Jean G, Kahles A, Bohnert R, Drewe P, Mudrakarta P, Görnitz N, Zeller G, Rätsch G. Oqtans: the RNA-seq workbench in the cloud for complete and reproducible quantitative transcriptome analysis. Bioinformatics 10.1093/bioinformatics/btt731 (2014) </help> </tool>