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view rDiff/mex/read.cpp @ 1:08d3a6143873
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author | vipints |
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date | Tue, 08 Oct 2013 06:54:25 -0400 |
parents | 0f80a5141704 |
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/* * This program is free software; you can redistribute it and/or modify * it under the terms of the GNU General Public License as published by * the Free Software Foundation; either version 3 of the License, or * (at your option) any later version. * * Written (W) 2010-2011 Jonas Behr, Regina Bohnert, Gunnar Raetsch * Copyright (C) 2010-2011 Max Planck Society */ #include "read.h" CRead::CRead() { read_id = NULL; sam_line = NULL; start_pos = 0; matches = 0; mismatches = 0; multiple_alignment_index = 0; strand = NULL; left = false; right = false; reverse = false; } CRead::~CRead() { delete[] read_id; delete[] sam_line; delete[] strand; } /* * Augments 'coverage' array at the positions covered by the read in the queried interval. */ void CRead::get_coverage(int p_start_pos, int p_end_pos, uint32_t* coverage) { // block1 block2 // |=====|======|============|===========|======|====| // ^ ^ ^ // p_start_pos | p_end_pos // start_pos // |0000001111111111111000000000000111111100000| // *coverage int len = p_end_pos-p_start_pos+1; for (uint32_t n = 0; n < block_starts.size(); n++) { int32_t from, to; from = block_starts[n]+start_pos-p_start_pos; to = block_starts[n]+start_pos-p_start_pos+block_lengths[n]; if (from < 0) from = 0; if (to < 0) continue; else if (to > len) to = len; for (int bp=from; bp<to; bp++) { coverage[bp]++; } } } int CRead::get_last_position() { if (block_starts.size()>0) // this if for some reason zero in case of softclips return start_pos+block_starts.back()+block_lengths.back(); return -1; } /* * Adds the column indices (= positions) covered by the read to 'reads' array in current row (= read). * These indices can be used to build up a sparse matrix of reads x positions. */ void CRead::get_reads_sparse(int p_start_pos, int p_end_pos, double* reads, uint32_t & reads_c, uint32_t row_idx) { int len = p_end_pos-p_start_pos+1; for (uint32_t n = 0; n < block_starts.size(); n++) { uint32_t from, to; if (block_starts[n]+start_pos-p_start_pos >= 0) from = block_starts[n]+start_pos-p_start_pos; else from = 0; if (block_starts[n]+start_pos-p_start_pos+block_lengths[n] >= 0) to = block_starts[n]+start_pos-p_start_pos+block_lengths[n]; else to = 0; for (int bp=from; bp<to&bp<len; bp++) { reads[reads_c] = row_idx+1; // row indices for sparse matrix reads[reads_c+1] = bp+1; // column indices for sparse matrix reads_c += 2; } } } void CRead::get_acc_splice_sites(vector<int>* acc_pos) { if (strand[0]=='+') { for (int k=1;k<block_starts.size(); k++) acc_pos->push_back(start_pos+block_starts[k]-1); } else if (strand[0]=='-') { for (int k=1;k<block_starts.size(); k++) acc_pos->push_back(start_pos+block_starts[k-1]+block_lengths[k-1]-2); } } void CRead::get_don_splice_sites(vector<int>* don_pos) { if (strand[0]=='+') { for (int k=1;k<block_starts.size(); k++) don_pos->push_back(start_pos+block_starts[k-1]+block_lengths[k-1]-2); } else if (strand[0]=='-') { for (int k=1;k<block_starts.size(); k++) don_pos->push_back(start_pos+block_starts[k]-1); } } int CRead::min_exon_len() { int min = 1e8; for (int k=0;k<block_starts.size(); k++) if (block_lengths[k]<min) min = block_lengths[k]; return min; } int CRead::max_intron_len() { int max = 0; for (int k=1;k<block_starts.size(); k++) if (block_starts[k]-(block_starts[k-1]+block_lengths[k-1])>max) max = block_starts[k]-(block_starts[k-1]+block_lengths[k-1]); return max; } /* * Adds start and end of introns in the read consecutively to the 'introns' vector. */ void CRead::get_introns(vector<int>* introns) { for (int i=1; i<block_starts.size(); i++) { int istart = block_starts[i-1]+block_lengths[i-1]+start_pos; int iend = block_starts[i]+start_pos-1; introns->push_back(istart); introns->push_back(iend); //fprintf(stdout, "%i intron: %d->%d\n", i, istart, iend); } } void CRead::get_introns(vector<uint32_t>* intron_starts, vector<uint32_t>* intron_ends, vector<uint32_t>* block_len1, vector<uint32_t>* block_len2) { for (int i=1; i<block_starts.size(); i++) { uint32_t istart = block_starts[i-1]+block_lengths[i-1]+start_pos; uint32_t iend = block_starts[i]+start_pos-1; intron_starts->push_back(istart); intron_ends->push_back(iend); block_len1->push_back(block_lengths[i-1]) ; block_len2->push_back(block_lengths[i]) ; } } bool CRead::operator==(const CRead& read) const { if (block_starts.size()!=read.block_starts.size()) return false; if (block_lengths.size()!=read.block_lengths.size()) return false; if (start_pos!=read.start_pos) return false; if (strand[0] != read.strand[0]) return false; for (int i=0; i<block_starts.size(); i++) if (block_starts[i]!=read.block_starts[i]) return false; for (int i=0; i<block_lengths.size(); i++) if (block_lengths[i]!=read.block_lengths[i]) return false; return true; } void CRead::print() { fprintf(stdout, "start_pos: %d\n", start_pos); fprintf(stdout, "starts:"); for (int i=0; i<block_starts.size(); i++) { fprintf(stdout, " %d", block_starts[i]); } fprintf(stdout, "\n"); fprintf(stdout, "lengths:"); for (int i=0; i<block_starts.size(); i++) { fprintf(stdout, " %d", block_lengths[i]); } fprintf(stdout, "\n"); } void CRead::set_strand(char s) { delete[] strand; strand = new char [2]; strand[0] = s; strand[1] = '0'; } int CRead::get_mismatches() { return mismatches ; }