Mercurial > repos > vipints > rdiff
view rDiff/src/tools/read_utils/get_reads_for_gene.m @ 0:0f80a5141704
version 0.3 uploaded
author | vipints |
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date | Thu, 14 Feb 2013 23:38:36 -0500 |
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function [reads1] = get_reads_for_gene(CFG,gene) % Get the reads from the bam-file if strcmp(gene.strand,'-') [mask1, read_intron_list] = get_reads(CFG.curr_bamfile, [CFG.chr_prefix gene.chr],gene.start+1,gene.stop+1, '0'); else [mask1, read_intron_list] = get_reads(CFG.curr_bamfile, [CFG.chr_prefix gene.chr],gene.start,gene.stop, '0'); end % Bring the reads into a matrix form if isempty(mask1) reads1=zeros(0,gene.stop-gene.start+1); return else reads1=sparse(mask1(1,:)',mask1(2,:)',ones(size(mask1,2),1),max(mask1(1,:)),gene.stop-gene.start+1); end % remove reads which are shorter than CFG.min_read_length reads1=reads1(sum(reads1,2)>CFG.min_read_length,:); if isempty(reads1) return end %remove reads which could stem from other genes [reads1,FLAG]=remove_reads_from_other_genes(reads1,gene); %Subsample reads if isempty(reads1) return end if CFG.rDiff_subsample>0 if size(reads1,1)>CFG.rDiff_subsample RP=randperm(size(reads1,1)); reads1=reads1(RP(1:CFG.rDiff_subsample),:); end end %Clip reads if isempty(reads1) return end if CFG.bases_to_clip>0 CFG.bases_to_clip=3; [reads1]=clip_reads(reads1,CFG.bases_to_clip); end