Mercurial > repos > wolma > mimodd
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author | wolma |
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date | Wed, 11 Feb 2015 08:57:11 -0500 |
parents | 6231ae8f87b8 |
children | 72d20758ba2c |
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<tool id="cloudmap_prepare" name="Prepare variant data for mapping"> <description>with the CloudMap series of tools.</description> <requirements> <requirement type="package">mimodd</requirement> </requirements> <version_command>mimodd version -q</version_command> <command> mimodd cloudmap "$ifile" ${run.mode} #if $str($run.mode) != "SVD": "${run.refsample}" #end if "$sample" -o "$ofile" #if $seqdict: -s "$dictfile" #end if </command> <inputs> <param name="ifile" type="data" format="vcf" label="vcf input file" /> <conditional name="run"> <param name="mode" type="select" label="CloudMap analysis to prepare data for"> <option value="SVD">EMS Variant Density Mapping</option> <option value="VAF">Variant Discovery / Hawaiian Variant Mapping</option> </param> <when value="SVD"> <param name="refsample" type="hidden" value="None" /> </when> <when value="VAF"> <param name="refsample" type="text" label="name of the reference sample" help="the sample that provides mapping strain variants" /> </when> </conditional> <param name="sample" type="text" label="subject sample name" help="the sample to perform CloudMap mapping for" /> <param name="seqdict" type="boolean" checked="true" label="Generate species configuration file for CloudMap" /> </inputs> <outputs> <data name="ofile" format="vcf" label="CloudMap-ready ${run.mode} File from ${on_string}" /> <data name="dictfile" format="tabular" label="Species Configuration File for CloudMap from ${on_string}"> <filter>seqdict</filter> </data> </outputs> <help> .. class:: infomark **What it does** The purpose of this tool is to provide compatibility of the MiModD analysis workflow with the external `CloudMap`_ *EMS Variant Density Mapping*, *Variant Discovery Mapping* and *Hawaiian Variant Mapping* tools. These tools complement MiModD by providing easily interpreted visualizations of mapping-by-sequencing analysis workflows. The tool converts a VCF file as generated by the *Extract Variant Sites* or *VCF Filter* tools to the format expected by the *CloudMap* series of tools. Optionally, it also extracts the chromosome names and sizes and reports them in the *CloudMap* *species configuration file* format. Such a file is required as input to the current versions of the *CloudMap* *Hawaiian* and *Variant Density* mapping tools, if you are working with a species other than the natively supported ones (i.e., other than *C. elegans* or *A. thaliana*). To use the output datasets of the tool with *CloudMap*, you only have to upload them to any public Galaxy server that hosts *CloudMap* like, e.g., the main Galaxy server at https://usegalaxy.org . .. class:: warningmark EMS Variant Density Mapping is currently limited to *C. elegans* and other species with six chromosomes on the *CloudMap* side. More information on combining MiModD and CloudMap in mapping-by-sequencing analyses can be found in the `corresponding section of the MiModD User Guide`_. .. _CloudMap: https://usegalaxy.org/u/gm2123/p/cloudmap .. _corresponding section of the MiModD User Guide: http://mimodd.readthedocs.org/en/latest/cloudmap.html </help> </tool>