Mercurial > repos > wolma > mimodd_bamsort
view bamsort.xml @ 0:099d2c4d8007 draft
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author | wolma |
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date | Sat, 13 Dec 2014 17:19:46 -0500 |
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<tool id="bamsort" name="Sort BAM file"> <description>Sort a BAM file by coordinates (or names) of the mapped reads</description> <requirements> <requirement type="package" version="0.1.5">mimodd</requirement> </requirements> <version_command>mimodd version -q</version_command> <command> mimodd sort $inputfile -o $output --oformat $oformat $by_name </command> <inputs> <param name="inputfile" type="data" format="bam" label="Input file to sort" /> <param name="by_name" type="boolean" truevalue = "-n" falsevalue ="" label="Sort by read names instead of coordinates" checked = "false" help="A less common option, but necessary, e.g., if you want to re-align sorted output from a previous run of the Snap Align Tool." /> <param name="oformat" type="boolean" truevalue = "sam" falsevalue = "bam" label = "Output in uncompressed SAM format" checked = "false" /> </inputs> <outputs> <data name="output" format="bam" label="Sorted output from MiModd ${tool.name} on ${on_string}"> <change_format> <when input="oformat" value="sam" format="sam" /> </change_format> </data> </outputs> <help> .. class:: infomark **What it does** The tool sorts a BAM file of aligned reads, typically by the reference genome coordinates that the reads have been mapped to. Coordinate-sorted input files are expected by most downstream MiModD tools, but note that the *SNAP Read Alignment* produces coordinate-sorted output by default and it is only necessary to sort files that come from other sources or from *SNAP Read Alignment* jobs with a custom sort order. The option *Sort by read names instead of coordinates* is useful if you want to re-align coordinate-sorted paired-end data. In *paired-end mode*, the *SNAP Read Alignment* tool expects the reads in the input file to be arranged in read pairs, i.e., the forward read information of a pair must be followed immediately by its reverse mate information, which is typically not the case in coordinate-sorted files. Resorting such files by read names fixes this problem. </help> </tool>