Mercurial > repos > xuebing > kplogo
diff kplogo.xml @ 0:f6226f8823ce draft
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author | xuebing |
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date | Thu, 23 Mar 2017 00:47:36 -0400 |
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children | 3c13235a05f5 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/kplogo.xml Thu Mar 23 00:47:36 2017 -0400 @@ -0,0 +1,102 @@ +<tool id="kplogo" name="kpLogo" version="1.0"> + <description>for positional k-mer analysis</description> + <command> + + kpLogo $input $inputtype -o $output -alphabet $alphabet $maxk -startPos $startPos $degenerate 2> tmp; + cp ${output}.most.significant.each.position.pdf $output; + cp ${output}.pdf $logo_prob; + cp ${output}.info.pdf $logo_info; + cp ${output}.freq.pdf $logo_freq; + cp ${output}.most.significant.each.position.txt $tab_most; + cp ${output}.pass.p.cutoff.txt $tab_all; + + </command> + <inputs> + <page> + <param format="fasta" name="input" type="data" label="Fasta File" /> + + <param name="alphabet" type="select" label="Sequence type: DNA/RNA or Protein" > + <option value="dna" selected="True">DNA/RNA</option> + <option value="protein">Protein</option> + <option value="other">Other (not supported yet)</option> + </param> + + <param name="inputtype" type="select" label="Weighted or Ranked" > + <option value="" selected="True">Unweighted (FASTA or Tab-delimited) </option> + <option value="-ranked">Ranked (FASTA or Tab-delimited)</option> + <option value="-weighted">Weighted (Tab-delimited)</option> + </param> + + <param name="maxk" type="select" label="k-mer length" + help="k-mer length"> + <option value="-max_k 4" selected="True">1,2,3,4</option> + <option value="-max_k 3">1,2,3</option> + <option value="-max_k 2">1,2</option> + <option value="-k 4">4</option> + <option value="-k 3">3</option> + <option value="-k 2">2</option> + <option value="-k 1">1</option> + </param> + + <param name="degenerate" type="select" label="Degenerate residual (DNA/RNA only)"> + <option value="-gapped" selected="True">ACGTN, i.e. gapped</option> + <option value="">None (no degenerate residuals)</option> + <option value="-degenerate all">all IUPAC residuals: ACGTRYMKWSBDHVN</option> + </param> + + <param name="startPos" type="integer" value="1"/> + + + </page> + </inputs> + <outputs> + <data format="pdf" name="logo_info" label="${tool.name} on ${on_string}: info"/> + <data format="pdf" name="logo_freq" label="${tool.name} on ${on_string}: freq"/> + <data format="pdf" name="logo_prob" label="${tool.name} on ${on_string}: prob"/> + <data format="pdf" name="output" label="${tool.name} on ${on_string}: kmer"/> + <data format="tabular" name="tab_all" label="${tool.name} on ${on_string}: all_kmer"/> + <data format="tabular" name="tab_most" label="${tool.name} on ${on_string}: most_sig"/> + + + </outputs> + <help> + +**Note** + +This tool uses Weblogo3_ in Galaxy to generate a sequence logo. The input file must be a fasta file in your current history. + +It is recommended for (eg) viewing multiple sequence alignments output from the clustalw tool - set the output to fasta and feed +it in to this tool. + +A typical output looks like this + +.. image:: ${static_path}/images/rgWebLogo3_test.jpg + +---- + +**Warning about input Fasta format files** + +The Weblogo3 program used by this tool will fail if your fasta sequences are not all EXACTLY the same length. The tool will provide a warning +and refuse to call the weblogo3 executable if irregular length sequences are detected. + +Fasta alignments from the companion ClustalW Galaxy tool will work but many other fasta files may cause this tool to fail - please do not file +a Galaxy bug report - this is a feature of the tool and a problem with your source data - not a tool error - please make certain all your fasta +sequences are the same length! + +---- + +**Attribution** + +Weblogo attribution and associated documentation are available at Weblogo3_ + +This Galaxy wrapper was written by Ross Lazarus for the rgenetics project and the source code is licensed under the LGPL_ like other rgenetics artefacts + +.. _Weblogo3: http://weblogo.berkeley.edu/ + +.. _LGPL: http://www.gnu.org/copyleft/lesser.html + + </help> + +</tool> + +