Mercurial > repos > xuebing > sharplab_seq_motif
view mytools/match.xml @ 5:84e2c77a6abd
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author | xuebing |
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date | Fri, 16 Mar 2012 14:01:16 -0400 |
parents | 7cc64021dc90 |
children | 7e14dac65e49 |
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<tool id="match" name="match"> <description>find short motif occurrences</description> <command> ./match $motif $seq $output $nmismatch $rc $bed > $log</command> <inputs> <param name="motif" type="data" format="fasta" label="Search occurances of the following motifs" help="one or multiple sequences, FASTA format"/> <param name="seq" type="data" format="fasta" label="in sequences" help="one or multiple sequences, FASTA format"/> <param name="nmismatch" size="10" type="integer" value="0" label="number of mismatches allowed"/> <param name="rc" label="Do not score the reverse complement DNA strand. Both strands are scored by default" type="boolean" truevalue="norc" falsevalue="rc" checked="False"/> <param name="bed" label="output BED format" help="Default is tabular file (see example below). Please only check this if your sequence identifier looks like: hg18_chr6_122208322_122209078_+ (true for galaxy tool extracted sequences)" type="boolean" truevalue="bed" falsevalue="nobed" checked="True"/> </inputs> <outputs> <data format="log" name="log" label="${tool.name} on ${on_string} (log)"/> <data format="bed" name="outfile" label="${tool.name} on ${on_string} (bed)"/> </outputs> <help> **What it does** This tool searches occurrences of a short nucleotide seuqences (allowing mismatches) in a set of longer sequences. Example motif file:: >motif1 CAGGTAAGT >motif2 GTTTGGGGGCC Example sequence file:: >hg18_chr6_122208322_122209078_+ CGTCGTAGCTACTAGCTACGTACGTACGTAGCTAGCATGCATGCTACGTA CGTAGCTAGCTAAAAAAAAAAAAAAACTGCGGCTAGCTAGCTAGCTACGT CGATCGTAGCTAC... >hg18_chr6_1208322_122209023_+ CGATGCTAGCTAGCTAGCTACGTAGCTAGCTAGTCGATGCTAGCTAGCTA ATGCTAGCTAGC.... Output (bed):: chr11 72790893 72790902 ACTTAACTG 1 - antisense 5ss,G4T:CAGTTAAGT-rc hg18_chr11_727 90846_72791902_+ 47 chr11 72791880 72791889 CAGGTAAGA 1 + sense 5ss,T9A:CAGGTAAGA hg18_chr11_72790846_72 791902_+ 1034 Output (tab):: Tmod4 802 5ss:CAGGTAAGT-rc ACTTACCTG Atp7b 77 5ss:CAGGTAAGT CAGGTAAGT Fnta 665 5ss:CAGGTAAGT CAGGTAAGT </help> </tool>