0
|
1
|
|
2 caloffset = function(genome){
|
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3 total_len = sum(as.numeric(genome[,2]))
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4 offset = 0
|
|
5 for (i in 1:nrow(genome)) {
|
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6 offset = c(offset,offset[i]+genome[i,2])
|
|
7 }
|
|
8 offset
|
|
9 }
|
|
10
|
|
11 coverage = function(intervals,genome,offset,resolution) {
|
|
12
|
|
13 nChr = length(offset) - 1
|
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14 total_len = offset[nChr+1]
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|
15 nbin = as.integer(total_len / resolution)
|
|
16 cov = numeric(nbin)#coverage
|
|
17 col = numeric(nbin)#color
|
|
18 for (i in 1:nChr) {
|
|
19 d = x[x[,1]==as.character(genome[i,1]),2:3]
|
|
20 d = ceiling(((d[,1]+d[,2])/2+offset[i])*nbin/total_len)
|
|
21 t = table(d)
|
|
22 pos = as.numeric(row.names(t))
|
|
23 cov[pos] = cov[pos] + as.numeric(t)
|
|
24 col[pos] = i
|
|
25 }
|
|
26 list(nbin=nbin,cov=cov,col=col)
|
|
27 }
|
|
28
|
|
29 # plot coverage
|
|
30 # res = genomeView(x,genome,100000)
|
|
31 plotcov = function(res,genome,offset,title,uselog) {
|
|
32 if (uselog == 'log'){
|
|
33 res$cov = log10(res$cov+1)
|
|
34 }
|
|
35 ymax = max(res$cov)
|
|
36 #print(ymax)
|
|
37 par(mar=c(5,5,5,1))
|
|
38 plot(seq(length(res$cov)),res$cov,type='h',cex=0.1,cex.axis=2,cex.lab=2,cex.main=3,col=res$col,xaxt='n',main=title,xlab='chromosome',ylab='coverage',frame.plot=F,ylim=c(0,ymax))
|
|
39 xticks = numeric(nrow(genome))
|
|
40 for (i in 1:nrow(genome)){
|
|
41 xticks[i] = (offset[i]+offset[i+1])/2*res$nbin/offset[length(offset)]
|
|
42 }
|
|
43 mtext(genome[,1],side=1,at=xticks,adj=1,las=2,col=seq(nrow(genome)))
|
|
44 }
|
|
45
|
|
46 union_correlation = function(x,y){
|
|
47 z = x>0 | y>0
|
|
48 cor(x[z],y[z])
|
|
49 }
|
|
50
|
|
51
|
|
52 heatmap2 = function(x,scale,sym,margins,labRow,labCol){
|
|
53 h = heatmap(x,scale=scale,sym=sym,margins=margins,labRow=labRow,labCol=labCol)
|
|
54 x = x[h$rowInd,h$colInd]
|
|
55 tx = numeric(0)
|
|
56 ty = numeric(0)
|
|
57 txt = character(0)
|
|
58 for (i in 1:nrow(x)){
|
|
59 for (j in 1:ncol(x)){
|
|
60 tx <- c(tx,i)
|
|
61 ty <- c(ty,ncol(x)-j+1)
|
|
62 txt <- c(txt,format(x[i,j],digits=2,nsmall=2))
|
|
63 }
|
|
64 }
|
|
65 #heatmap(x,scale=scale,sym=sym,margins=margins,labRow=labRow[h$rowInd],labCol=labCol[h$colInd],add.expr=text(1:4,1:4,1:4))
|
|
66 cat(dim(tx))
|
|
67 text(tx,ty,txt)
|
|
68 heatmap(x,scale=scale,sym=sym,margins=margins,labRow=labRow[h$rowInd],labCol=labCol[h$colInd],add.expr=text(tx,ty,txt))
|
|
69
|
|
70 }
|