0
|
1
|
|
2 caloffset = function(genome){
|
|
3 total_len = sum(as.numeric(genome[,2]))
|
|
4 offset = 0
|
|
5 for (i in 1:nrow(genome)) {
|
|
6 offset = c(offset,offset[i]+genome[i,2])
|
|
7 }
|
|
8 offset
|
|
9 }
|
|
10
|
|
11 coverage = function(intervals,genome,offset,resolution) {
|
|
12
|
|
13 nChr = length(offset) - 1
|
|
14 total_len = offset[nChr+1]
|
|
15 nbin = as.integer(total_len / resolution)
|
|
16
|
|
17 pos = numeric(0)
|
|
18 cov = numeric(0)#coverage
|
|
19 col = numeric(0)#color
|
|
20 for (i in 1:nChr) {
|
|
21 d = x[x[,1]==as.character(genome[i,1]),2:3]
|
|
22 d = ceiling(((d[,1]+d[,2])/2+offset[i])*nbin/total_len)
|
|
23 t = table(d)
|
|
24 pos = c(pos,as.numeric(row.names(t)))
|
|
25 cov = c(cov, as.numeric(t))
|
|
26 col = c(col,numeric(length(t))+i)
|
|
27 }
|
|
28 list(nbin=nbin,pos=pos,cov=cov,col=col)
|
|
29 }
|
|
30
|
|
31 # plot coverage
|
|
32 # res = genomeView(x,genome,100000)
|
|
33 plotcov = function(res,genome,offset,title,uselog) {
|
|
34 if (uselog == 'log'){
|
|
35 res$cov = log10(res$cov+1)
|
|
36 }
|
|
37 ymax = max(res$cov)
|
|
38 par(mar=c(5,5,5,1))
|
|
39 plot(res$pos,res$cov,type='h',cex=0.1,cex.axis=2,cex.lab=2,cex.main=3,col=res$col,xaxt='n',main=title,xlab='chromosome',ylab='coverage',frame.plot=F,xlim=c(0,res$nbin),ylim=c(0,ymax))
|
|
40 xticks = numeric(nrow(genome))
|
|
41 for (i in 1:nrow(genome)){
|
|
42 xticks[i] = (offset[i]+offset[i+1])/2*res$nbin/offset[length(offset)]
|
|
43 }
|
|
44 mtext(genome[,1],side=1,at=xticks,adj=1,las=2,col=seq(nrow(genome)))
|
|
45 }
|