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1 #!/usr/bin/env python
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2 # Greg Von Kuster
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3
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4 """
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5 Subtract an entire query from another query
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6 usage: %prog in_file_1 in_file_2 begin_col end_col output
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7 """
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8 import sys, re
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9 from galaxy import eggs
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10 import pkg_resources; pkg_resources.require( "bx-python" )
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11 from bx.cookbook import doc_optparse
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12
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13 # Older py compatibility
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14 try:
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15 set()
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16 except:
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17 from sets import Set as set
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18
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19 assert sys.version_info[:2] >= ( 2, 4 )
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20
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21 def get_lines(fname, begin_col='', end_col=''):
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22 lines = set([])
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23 i = 0
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24 for i, line in enumerate(file(fname)):
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25 line = line.rstrip('\r\n')
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26 if line and not line.startswith('#'):
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27 if begin_col and end_col:
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28 """Both begin_col and end_col must be integers at this point."""
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29 try:
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30 line = line.split('\t')
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31 line = '\t'.join([line[j] for j in range(begin_col-1, end_col)])
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32 lines.add( line )
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33 except: pass
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34 else:
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35 lines.add( line )
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36 if i: return (i+1, lines)
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37 else: return (i, lines)
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38
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39 def main():
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40
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41 # Parsing Command Line here
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42 options, args = doc_optparse.parse( __doc__ )
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43
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44 try:
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45 inp1_file, inp2_file, begin_col, end_col, out_file = args
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46 except:
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47 doc_optparse.exception()
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48
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49 begin_col = begin_col.strip()
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50 end_col = end_col.strip()
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51
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52 if begin_col != 'None' or end_col != 'None':
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53 """
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54 The user selected columns for restriction. We'll allow default
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55 values for both begin_col and end_col as long as the user selected
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56 at least one of them for restriction.
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57 """
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58 if begin_col == 'None':
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59 begin_col = end_col
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60 elif end_col == 'None':
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61 end_col = begin_col
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62 begin_col = int(begin_col)
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63 end_col = int(end_col)
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64 """Make sure that begin_col <= end_col (switch if not)"""
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65 if begin_col > end_col:
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66 tmp_col = end_col
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67 end_col = begin_col
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68 begin_col = tmp_col
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69 else:
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70 begin_col = end_col = ''
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71
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72 try:
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73 fo = open(out_file,'w')
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74 except:
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75 print >> sys.stderr, "Unable to open output file"
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76 sys.exit()
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77
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78 """
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79 len1 is the number of lines in inp1_file
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80 lines1 is the set of unique lines in inp1_file
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81 diff1 is the number of duplicate lines removed from inp1_file
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82 """
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83 len1, lines1 = get_lines(inp1_file, begin_col, end_col)
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84 diff1 = len1 - len(lines1)
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85 len2, lines2 = get_lines(inp2_file, begin_col, end_col)
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86
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87 lines1.difference_update(lines2)
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88 """lines1 is now the set of unique lines in inp1_file - the set of unique lines in inp2_file"""
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89
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90 for line in lines1:
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91 print >> fo, line
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92
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93 fo.close()
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94
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95 info_msg = 'Subtracted %d lines. ' %((len1 - diff1) - len(lines1))
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96
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97 if begin_col and end_col:
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98 info_msg += 'Restricted to columns c' + str(begin_col) + ' thru c' + str(end_col) + '. '
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99
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100 if diff1 > 0:
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101 info_msg += 'Eliminated %d duplicate/blank/comment/invalid lines from first query.' %diff1
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102
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103 print info_msg
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104
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105 if __name__ == "__main__":
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106 main()
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