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1 <tool id="EMBOSS: trimest102" name="trimest" version="5.0.0">
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2 <description>Trim poly-A tails off EST sequences</description>
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3 <requirements><requirement type="package" version="5.0.0">emboss</requirement></requirements>
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4 <command>trimest -sequence $input1 -outseq $out_file1 -minlength "$minlength" -mismatches "$mismatches" -reverse $reverse -tolower $tolower -fiveprime $fiveprime -osformat2 $out_format1
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5 -auto</command>
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6 <inputs>
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7 <param format="fasta" name="input1" type="data">
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8 <label>Sequences</label>
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9 </param>
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10 <param name="minlength" size="4" type="text" value="4">
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11 <label>Minimum length that a poly-A (or poly-T) tail must have before it is removed</label>
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12 </param>
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13 <param name="mismatches" size="4" type="text" value="1">
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14 <label>Number of fewer mismatched non-A bases in a poly-A tail</label>
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15 </param>
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16 <param name="reverse" type="select">
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17 <label>Change the sequence to the forward sense when it is written out</label>
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18 <option value="yes">Yes</option>
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19 <option value="no">No</option>
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20 </param>
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21 <param name="tolower" type="select">
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22 <label>Mask poly-A by converting to lowercase</label>
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23 <option value="no">No</option>
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24 <option value="yes">Yes</option>
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25 </param>
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26 <param name="fiveprime" type="select">
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27 <label>Inspect 5' end of the sequence for poly-T tails</label>
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28 <option value="yes">Yes</option>
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29 <option value="no">No</option>
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30 </param>
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31 <param name="out_format1" type="select">
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32 <label>Output Sequence File Format</label>
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33 <option value="fasta">FASTA (m)</option>
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34 <option value="acedb">ACeDB (m)</option>
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35 <option value="asn1">ASN.1 (m)</option>
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36 <option value="clustal">Clustal (m)</option>
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37 <option value="codata">CODATA (m)</option>
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38 <option value="embl">EMBL (m)</option>
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39 <option value="fitch">Fitch (m)</option>
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40 <option value="gcg">Wisconsin Package GCG 9.x and 10.x (s)</option>
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41 <option value="genbank">GENBANK (m)</option>
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42 <option value="gff">GFF (m)</option>
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43 <option value="hennig86">Hennig86 (m)</option>
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44 <option value="ig">Intelligenetics (m)</option>
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45 <option value="jackknifer">Jackknifer (m)</option>
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46 <option value="jackknifernon">Jackknifernon (m)</option>
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47 <option value="mega">Mega (m)</option>
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48 <option value="meganon">Meganon (m)</option>
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49 <option value="msf">Wisconsin Package GCG's MSF (m)</option>
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50 <option value="pir">NBRF (PIR) (m)</option>
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51 <option value="ncbi">NCBI style FASTA (m)</option>
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52 <option value="nexus">Nexus/PAUP (m)</option>
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53 <option value="nexusnon">Nexusnon/PAUPnon (m)</option>
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54 <option value="phylip">PHYLIP interleaved (m)</option>
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55 <option value="phylipnon">PHYLIP non-interleaved (m)</option>
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56 <option value="selex">SELEX (m)</option>
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57 <option value="staden">Staden (s)</option>
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58 <option value="strider">DNA strider (m)</option>
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59 <option value="swiss">SwisProt entry (m)</option>
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60 <option value="text">Plain sequence (s)</option>
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61 <option value="treecon">Treecon (m)</option>
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62 </param>
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63 </inputs>
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64 <outputs>
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65 <data format="fasta" name="out_file1" />
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66 </outputs>
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67 <tests>
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68 <test>
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69 <param name="input1" value="2.fasta"/>
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70 <param name="minlength" value="4"/>
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71 <param name="mismatches" value="1"/>
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72 <param name="reverse" value="yes"/>
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73 <param name="tolower" value="no"/>
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74 <param name="fiveprime" value="yes"/>
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75 <param name="out_format1" value="fasta"/>
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76 <output name="out_file1" file="emboss_trimest_out.fasta"/>
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77 </test>
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78 </tests>
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79 <code file="emboss_format_corrector.py" />
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80 <help>
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81
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82 .. class:: warningmark
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83
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84 The input dataset needs to be sequences.
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85
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86 -----
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87
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88 You can view the original documentation here_.
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89
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90 .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/trimest.html
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91 </help>
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92 </tool>
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