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1 '''
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2 get binned score of intervals,allow extension
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3 '''
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4
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5 import os,sys,numpy
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6 import string, random
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7
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8 from resize import *
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9
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10 from bx.bbi.bigwig_file import BigWigFile
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11
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12 def binning(x,n):
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13 # make n bin of x
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14 y = numpy.zeros(n,dtype=float)
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15 if len(x) == 0:
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16 return y
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17 step = float(len(x))/n
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18 for k in range(n):
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19 i = int(step*k)
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20 j = int(step*(k+1)) + 1
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21 y[k] = x[i:j].mean()
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22 #print i,j,k,y[k]
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23 return y
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24
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25 def getBinnedScore(bwfile,intvfile,nbin):
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26 '''
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27 get binned average and std
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28 '''
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29 fbw = open(bwfile)
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30 bw = BigWigFile(file=fbw)
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31 fin = open(intvfile)
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32 avg = numpy.zeros(nbin)
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33 sqr = numpy.zeros(nbin)
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34 N = 0
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35 for line in fin:
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36 #print N
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37 #chrom,start,end,name,score,strand
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38 flds = line.strip().split('\t')
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39 #get the score at base resolution as an array
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40 scores = bw.get_as_array(flds[0],int(flds[1]),int(flds[2]))
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41 if scores == None:
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42 print 'not found:\t',N,line
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43 continue
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44 N = N + 1
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45 #print line,scores
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46 # reverse if on minus strand
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47 if flds[5] == '-':
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48 scores = scores[::-1]
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49 # no score = 0
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50 scores = numpy.nan_to_num(scores)
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51 # bin the data
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52 binned = binning(scores,nbin)
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53 avg = avg + binned
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54 sqr = sqr + binned**2
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55 # compute avg and std
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56 avg = avg / N
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57 err = ((sqr/N-avg**2)**0.5)/(N**0.5)
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58 return avg,err,N
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59
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60 def getExtendedBinScore(bwfile,intvfile,nbins,exts):
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61 '''
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62 nbins: n1,n2,n3
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63 exts: l1,l2,l3,l4
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64 '''
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65 avg = []
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66 err = []
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67 tmpfile = "".join(random.sample(string.letters+string.digits, 8))
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68 if exts[0]>0 or exts[1]>0:
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69 print 'make left extension'
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70 resize(intvfile,tmpfile,'start-'+str(exts[0]),'start+'+str(exts[1]),'stranded')
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71 print 'compute binned average'
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72 avg,err,N = getBinnedScore(bwfile,tmpfile,nbins[0])
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73 print 'regions used:',N
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74 print 'make center region'
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75 resize(intvfile,tmpfile,'start+'+str(exts[1]),'end-'+str(exts[2]),'stranded')
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76 print 'compute binned average'
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77 avg1,err1,N = getBinnedScore(bwfile,tmpfile,nbins[1])
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78 print 'regions used:',N
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79 avg = numpy.concatenate((avg,avg1))
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80 err = numpy.concatenate((err,err1))
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81 if exts[2]>0 or exts[3]>0:
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82 print 'make right region'
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83 resize(intvfile,tmpfile,'end-'+str(exts[2]),'end+'+str(exts[3]),'stranded')
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84 print 'compute binned average'
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85 avg2,err2,N = getBinnedScore(bwfile,tmpfile,nbins[2])
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86 print 'regions used:',N
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87 avg = numpy.concatenate((avg,avg2))
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88 err = numpy.concatenate((err,err2))
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89 os.system('rm '+tmpfile)
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90 return avg,err
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91
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92 prog,bwfile,intvfile,exts,nbins,outfile,outplot = sys.argv
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93 nbins = numpy.fromstring(nbins,dtype=int,sep=',')
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94 exts = numpy.fromstring(exts,dtype=int,sep=',')
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95 avg, err = getExtendedBinScore(bwfile,intvfile,nbins,exts)
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96 print 'save data'
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97 out = open(outfile,'w')
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98 numpy.savetxt(out, avg, fmt='%.6f', delimiter=' ', newline=' ')
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99 out.write('\n')
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100 numpy.savetxt(out, err, fmt='%.6f', delimiter=' ', newline=' ')
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101 out.write('\n')
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102 out.close()
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103
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104 print 'plot'
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105 start = exts[0]*nbins[0]/(exts[0]+exts[1])
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106 end = nbins[0]+nbins[1]+exts[2]*nbins[2]/(exts[2]+exts[3])
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107 #print start,end
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108 rscript = open("tmp.r","w")
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109 rscript.write("options(warn=-1)\n")
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110 rscript.write("x <- read.table('"+outfile+"')\n")
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111 rscript.write("pdf('"+outplot+"')\n")
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112 rscript.write("avg <- x[1,]\n")
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113 rscript.write("err <- x[2,]\n")
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114 #rscript.write("print(x)\n")
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115 rscript.write("ylim=c(min(avg-err),max(avg+err))\n")
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116 rscript.write("xticks <- seq(ncol(x))\n")
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117 #rscript.write("print(xticks)\n")
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118 rscript.write("plot(xticks,avg,xlab='',ylab='average coverage',type='l',lwd=0,ylim=ylim,xaxt='n')\n")
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119 rscript.write("axis(1, at=c(min(xticks),"+str(start)+","+str(end)+",max(xticks)),labels=c(-"+str(exts[0])+",0,0,"+str(exts[3])+"), las=2)\n")
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120 rscript.write("polygon(c(xticks,rev(xticks)),c(avg+err,rev(avg-err)),col='lightgreen',border=NA)\n")
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121 rscript.write("lines(xticks,avg,type='l',lwd=1)\n")
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122 rscript.write("lines(c(min(xticks),max(xticks)),c(0,0),lwd=2)\n")
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123 rscript.write("lines(c("+str(start)+","+str(end)+"),c(0,0),lwd=10)\n")
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124 rscript.write("dev.off()\n")
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125 rscript.close()
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126 os.system("R --vanilla --slave < tmp.r")
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127 os.system("rm tmp.r")
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128
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