comparison tools/emboss_5/emboss_est2genome.xml @ 0:9071e359b9a3

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author xuebing
date Fri, 09 Mar 2012 19:37:19 -0500
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1 <tool id="EMBOSS: est2genome32" name="est2genome" version="5.0.0">
2 <description>Align EST and genomic DNA sequences</description>
3 <requirements><requirement type="package" version="5.0.0">emboss</requirement></requirements>
4 <command>est2genome -estsequence $input1 -genomesequence $input2 -outfile $out_file1 -match $match -mismatch $mismatch -gappenalty $gappenalty -intronpenalty $intronpenalty -splicepenalty
5 $splicepenalty -minscore $minscore -reverse $reverse -splice $splice -mode $mode -best $best -shuffle $shuffle -seed $seed -align $align -width $width -auto</command>
6 <inputs>
7 <param format="fasta" name="input1" type="data">
8 <label>EST sequence(s)</label>
9 </param>
10 <param format="data" name="input2" type="data">
11 <label>Genomic sequence</label>
12 </param>
13 <param name="match" size="4" type="text" value="1">
14 <label>Score for matching two bases</label>
15 </param>
16 <param name="mismatch" size="4" type="text" value="1">
17 <label>Cost for mismatching two bases</label>
18 </param>
19 <param name="gappenalty" size="4" type="text" value="2">
20 <label>Cost for deleting a single base in either sequence, excluding introns</label>
21 </param>
22 <param name="intronpenalty" size="4" type="text" value="40">
23 <label>Cost for an intron, independent of length</label>
24 </param>
25 <param name="splicepenalty" size="4" type="text" value="20">
26 <label>Cost for an intron, independent of length and starting/ending on donor-acceptor sites</label>
27 </param>
28 <param name="minscore" size="4" type="text" value="30">
29 <label>Exclude alignments with scores below this threshold score</label>
30 </param>
31 <param name="reverse" type="select">
32 <label>Reverse the orientation of the EST sequence</label>
33 <option value="no">No</option>
34 <option value="yes">Yes</option>
35 </param>
36 <param name="splice" type="select">
37 <label>Use donor and acceptor splice sites</label>
38 <option value="yes">Yes</option>
39 <option value="no">No</option>
40 </param>
41 <param name="mode" type="select">
42 <label>Comparison mode</label>
43 <option value="both">Both strands</option>
44 <option value="forward">Forward strand only</option>
45 <option value="reverse">Reverse strand only</option>
46 </param>
47 <param name="best" type="select">
48 <label>Only best comparisons</label>
49 <option value="yes">Yes</option>
50 <option value="no">No</option>
51 </param>
52 <param name="shuffle" size="4" type="text" value="0">
53 <label>Shuffle</label>
54 </param>
55 <param name="seed" size="4" type="text" value="20825">
56 <label>Random number seed</label>
57 </param>
58 <param name="align" type="select">
59 <label>Show the alignment</label>
60 <option value="no">No</option>
61 <option value="yes">Yes</option>
62 </param>
63 <param name="width" size="4" type="text" value="50">
64 <label>Alignment width</label>
65 </param>
66 </inputs>
67 <outputs>
68 <data format="est2genome" name="out_file1" />
69 </outputs>
70 <tests>
71 <test>
72 <param name="input1" value="2.fasta"/>
73 <param name="input2" value="1.fasta"/>
74 <param name="match" value="1"/>
75 <param name="mismatch" value="1"/>
76 <param name="match" value="1"/>
77 <param name="gappenalty" value="2"/>
78 <param name="intronpenalty" value="40"/>
79 <param name="splicepenalty" value="20"/>
80 <param name="minscore" value="30"/>
81 <param name="reverse" value="no"/>
82 <param name="splice" value="yes"/>
83 <param name="mode" value="both"/>
84 <param name="best" value="yes"/>
85 <param name="shuffle" value="0"/>
86 <param name="seed" value="20825"/>
87 <param name="align" value="no"/>
88 <param name="width" value="50"/>
89 <output name="out_file1" file="emboss_est2genome_out.est2genome"/>
90 </test>
91 </tests>
92 <help>
93 .. class:: warningmark
94
95 The input dataset needs to be sequences.
96
97 -----
98
99 You can view the original documentation here_.
100
101 .. _here: http://emboss.sourceforge.net/apps/release/5.0/emboss/apps/est2genome.html
102 </help>
103 </tool>