Mercurial > repos > xuebing > sharplabtool
diff tools/fastq/fastq_manipulation.py @ 0:9071e359b9a3
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author | xuebing |
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date | Fri, 09 Mar 2012 19:37:19 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/fastq/fastq_manipulation.py Fri Mar 09 19:37:19 2012 -0500 @@ -0,0 +1,37 @@ +#Dan Blankenberg +import sys, os, shutil +import imp +from galaxy_utils.sequence.fastq import fastqReader, fastqWriter + +def main(): + #Read command line arguments + input_filename = sys.argv[1] + script_filename = sys.argv[2] + output_filename = sys.argv[3] + additional_files_path = sys.argv[4] + input_type = sys.argv[5] or 'sanger' + + #Save script file for debuging/verification info later + os.mkdir( additional_files_path ) + shutil.copy( script_filename, os.path.join( additional_files_path, 'debug.txt' ) ) + + fastq_manipulator = imp.load_module( 'fastq_manipulator', open( script_filename ), script_filename, ( '', 'r', imp.PY_SOURCE ) ) + + out = fastqWriter( open( output_filename, 'wb' ), format = input_type ) + + i = None + reads_manipulated = 0 + for i, fastq_read in enumerate( fastqReader( open( input_filename ), format = input_type ) ): + new_read = fastq_manipulator.match_and_manipulate_read( fastq_read ) + if new_read: + out.write( new_read ) + if new_read != fastq_read: + reads_manipulated += 1 + out.close() + if i is None: + print "Your file contains no valid FASTQ reads." + else: + print 'Manipulated %s of %s reads (%.2f%%).' % ( reads_manipulated, i + 1, float( reads_manipulated ) / float( i + 1 ) * 100.0 ) + +if __name__ == "__main__": + main()