Mercurial > repos > xuebing > sharplabtool
diff tools/fastq/fastq_paired_end_deinterlacer.xml @ 0:9071e359b9a3
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author | xuebing |
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date | Fri, 09 Mar 2012 19:37:19 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/fastq/fastq_paired_end_deinterlacer.xml Fri Mar 09 19:37:19 2012 -0500 @@ -0,0 +1,70 @@ +<tool id="fastq_paired_end_deinterlacer" name="FASTQ de-interlacer" version="1.1"> + <description>on paired end reads</description> + <command interpreter="python">fastq_paired_end_deinterlacer.py '$input_file' '${input_file.extension[len( 'fastq' ):]}' '$output1_pairs_file' '$output2_pairs_file' '$output1_singles_file' '$output2_singles_file'</command> + <inputs> + <param name="input_file" type="data" format="fastqsanger,fastqcssanger" label="FASTQ reads" /> + </inputs> + <outputs> + <data name="output1_pairs_file" format="input" label="FASTQ de-interlacer left mates from data ${input_file.hid}" /> + <data name="output2_pairs_file" format="input" label="FASTQ de-interlacer right mates from data ${input_file.hid}"/> + <data name="output1_singles_file" format="input" label="FASTQ de-interlacer left singles from data ${input_file.hid}"/> + <data name="output2_singles_file" format="input" label="FASTQ de-interlacer right singles from data ${input_file.hid}"/> + </outputs> + <tests> + <test> + <param name="input_file" value="paired_end_merged.fastqsanger" ftype="fastqsanger" /> + <output name="output1_pairs_file" file="paired_end_1.fastqsanger" /> + <output name="output2_pairs_file" file="paired_end_2.fastqsanger" /> + <output name="output1_singles_file" file="paired_end_1_singles.fastqsanger" /> + <output name="output2_singles_file" file="paired_end_2_singles.fastqsanger" /> + </test> + <test> + <param name="input_file" value="paired_end_merged_errors.fastqsanger" ftype="fastqsanger" /> + <output name="output1_pairs_file" file="paired_end_1_cleaned.fastqsanger" /> + <output name="output2_pairs_file" file="paired_end_2_cleaned.fastqsanger" /> + <output name="output1_singles_file" file="paired_end_1_cleaned_singles.fastqsanger" /> + <output name="output2_singles_file" file="paired_end_2_cleaned_singles.fastqsanger" /> + </test> + </tests> + <help> +**What it does** + +De-interlaces a single fastq dataset representing paired-end run into two fastq datasets containing only the first or second mate read. Reads without mate are saved in separate output files. + +Sequence identifiers for paired-end reads must follow the /1 and /2 convention. + +----- + +**Input** + +A multiple-fastq file containing paired-end reads, for example:: + + @1539:931/1 + ACTTCCCGCGCGTGAAGGCGCCGGCAAACGAGGCTCGGGAAGGGGCTCCCG + +1539:931/1 + BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB + @1539:931/2 + CGCCATTCCGAATCGTAGTTGTCGGCGTCTTCCAGTGCGGCAAGGCATCGT + +1539:931/2 + WNUUZ\P^`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB + +----- + +**Output** + +Multi-fastq file with left-hand mate only:: + + @1539:931/1 + ACTTCCCGCGCGTGAAGGCGCCGGCAAACGAGGCTCGGGAAGGGGCTCCCG + +1539:931/1 + BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB + +Multi-fastq file with right-hand mate only:: + + @1539:931/2 + CGCCATTCCGAATCGTAGTTGTCGGCGTCTTCCAGTGCGGCAAGGCATCGT + +1539:931/2 + WNUUZ\P^`BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB + + </help> +</tool>