Mercurial > repos > xuebing > sharplabtool
diff tools/fastx_toolkit/fastx_reverse_complement.xml @ 0:9071e359b9a3
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author | xuebing |
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date | Fri, 09 Mar 2012 19:37:19 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/fastx_toolkit/fastx_reverse_complement.xml Fri Mar 09 19:37:19 2012 -0500 @@ -0,0 +1,63 @@ +<tool id="cshl_fastx_reverse_complement" name="Reverse-Complement"> + <description></description> + <requirements><requirement type="package">fastx_toolkit</requirement></requirements> + <command>zcat -f '$input' | fastx_reverse_complement -v -o $output +#if $input.ext == "fastqsanger": +-Q 33 +#end if + </command> + <inputs> + <param format="fasta,fastqsolexa,fastqsanger" name="input" type="data" label="Library to reverse-complement" /> + </inputs> + + <tests> + <test> + <!-- Reverse-complement a FASTA file --> + <param name="input" value="fastx_rev_comp1.fasta" /> + <output name="output" file="fastx_reverse_complement1.out" /> + </test> + <test> + <!-- Reverse-complement a FASTQ file --> + <param name="input" value="fastx_rev_comp2.fastq" ftype="fastqsolexa"/> + <output name="output" file="fastx_reverse_complement2.out" /> + </test> + </tests> + + + <outputs> + <data format="input" name="output" metadata_source="input" /> + </outputs> + +<help> +**What it does** + +This tool reverse-complements each sequence in a library. +If the library is a FASTQ, the quality-scores are also reversed. + +-------- + +**Example** + +Input FASTQ file:: + + @CSHL_1_FC42AGWWWXX:8:1:3:740 + TGTCTGTAGCCTCNTCCTTGTAATTCAAAGNNGGTA + +CSHL_1_FC42AGWWWXX:8:1:3:740 + 33 33 33 34 33 33 33 33 33 33 33 33 27 5 27 33 33 33 33 33 33 27 21 27 33 32 31 29 26 24 5 5 15 17 27 26 + + +Output FASTQ file:: + + @CSHL_1_FC42AGWWWXX:8:1:3:740 + TACCNNCTTTGAATTACAAGGANGAGGCTACAGACA + +CSHL_1_FC42AGWWWXX:8:1:3:740 + 26 27 17 15 5 5 24 26 29 31 32 33 27 21 27 33 33 33 33 33 33 27 5 27 33 33 33 33 33 33 33 33 34 33 33 33 + +------ + +This tool is based on `FASTX-toolkit`__ by Assaf Gordon. + + .. __: http://hannonlab.cshl.edu/fastx_toolkit/ + +</help> +</tool>