Mercurial > repos > xuebing > sharplabtool
diff tools/filters/bed2gff.xml @ 0:9071e359b9a3
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author | xuebing |
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date | Fri, 09 Mar 2012 19:37:19 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/filters/bed2gff.xml Fri Mar 09 19:37:19 2012 -0500 @@ -0,0 +1,89 @@ +<tool id="bed2gff1" name="BED-to-GFF" version="2.0.0"> + <description>converter</description> + <command interpreter="python">bed_to_gff_converter.py $input $out_file1</command> + <inputs> + <param format="bed" name="input" type="data" label="Convert this query"/> + </inputs> + <outputs> + <data format="gff" name="out_file1" /> + </outputs> + <tests> + <test> + <param name="input" value="9.bed"/> + <output name="out_file1" file="bed2gff_out.gff"/> + </test> + </tests> + <help> + +**What it does** + +This tool converts data from BED format to GFF format (scroll down for format description). + +-------- + +**Example** + +The following data in BED format:: + + chr28 346187 388197 BC114771 0 + 346187 388197 0 9 144,81,115,63,155,96,134,105,112, 0,24095,26190,31006,32131,33534,36994,41793,41898, + +Will be converted to GFF (**note** that the start coordinate is incremented by 1):: + + ##gff-version 2 + ##bed_to_gff_converter.py + + chr28 bed2gff mRNA 346188 388197 0 + . mRNA BC114771; + chr28 bed2gff exon 346188 346331 0 + . exon BC114771; + chr28 bed2gff exon 370283 370363 0 + . exon BC114771; + chr28 bed2gff exon 372378 372492 0 + . exon BC114771; + chr28 bed2gff exon 377194 377256 0 + . exon BC114771; + chr28 bed2gff exon 378319 378473 0 + . exon BC114771; + chr28 bed2gff exon 379722 379817 0 + . exon BC114771; + chr28 bed2gff exon 383182 383315 0 + . exon BC114771; + chr28 bed2gff exon 387981 388085 0 + . exon BC114771; + chr28 bed2gff exon 388086 388197 0 + . exon BC114771; + + +------ + +.. class:: informark + +**About formats** + +**BED format** Browser Extensible Data format was designed at UCSC for displaying data tracks in the Genome Browser. It has three required fields and several additional optional ones: + +The first three BED fields (required) are:: + + 1. chrom - The name of the chromosome (e.g. chr1, chrY_random). + 2. chromStart - The starting position in the chromosome. (The first base in a chromosome is numbered 0.) + 3. chromEnd - The ending position in the chromosome, plus 1 (i.e., a half-open interval). + +The additional BED fields (optional) are:: + + 4. name - The name of the BED line. + 5. score - A score between 0 and 1000. + 6. strand - Defines the strand - either '+' or '-'. + 7. thickStart - The starting position where the feature is drawn thickly at the Genome Browser. + 8. thickEnd - The ending position where the feature is drawn thickly at the Genome Browser. + 9. reserved - This should always be set to zero. + 10. blockCount - The number of blocks (exons) in the BED line. + 11. blockSizes - A comma-separated list of the block sizes. The number of items in this list should correspond to blockCount. + 12. blockStarts - A comma-separated list of block starts. All of the blockStart positions should be calculated relative to chromStart. The number of items in this list should correspond to blockCount. + 13. expCount - The number of experiments. + 14. expIds - A comma-separated list of experiment ids. The number of items in this list should correspond to expCount. + 15. expScores - A comma-separated list of experiment scores. All of the expScores should be relative to expIds. The number of items in this list should correspond to expCount. + +**GFF format** General Feature Format is a format for describing genes and other features associated with DNA, RNA and Protein sequences. GFF lines have nine tab-separated fields:: + + 1. seqname - Must be a chromosome or scaffold. + 2. source - The program that generated this feature. + 3. feature - The name of this type of feature. Some examples of standard feature types are "CDS", "start_codon", "stop_codon", and "exon". + 4. start - The starting position of the feature in the sequence. The first base is numbered 1. + 5. end - The ending position of the feature (inclusive). + 6. score - A score between 0 and 1000. If there is no score value, enter ".". + 7. strand - Valid entries include '+', '-', or '.' (for don't know/care). + 8. frame - If the feature is a coding exon, frame should be a number between 0-2 that represents the reading frame of the first base. If the feature is not a coding exon, the value should be '.'. + 9. group - All lines with the same group are linked together into a single item. + +</help> +</tool>