Mercurial > repos > xuebing > sharplabtool
diff tools/genetrack/genetrack_indexer.xml @ 0:9071e359b9a3
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author | xuebing |
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date | Fri, 09 Mar 2012 19:37:19 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/genetrack/genetrack_indexer.xml Fri Mar 09 19:37:19 2012 -0500 @@ -0,0 +1,54 @@ +<tool id="bed2genetrack" name="GeneTrack indexer" version="1.0.1"> + + <description>on a BED file</description> + + <command interpreter="python"> + genetrack_indexer.py -i $input -o $output -s $shift -v 0 -f BED -x + </command> + + <inputs> + + <param format="bed6" name="input" type="data" help="Input data"> + <label>Select input bed file</label> + </param> + + <param name="shift" size="4" type="integer" value="0" help="distance in basepairs"> + <label>Shift at 5' end</label> + </param> + + <!-- this parameter is currently not used, may not be feasible to use it + <param name="coverage" type="select" label="Full coverage"> + <option value="no">NO</option> + <option value="yes">YES</option> + </param> + --> + + </inputs> + + <outputs> + <data format="genetrack" name="output" /> + </outputs> + +<help> +**Help** + +This tool will create a visualization of the bed file that is selected. + +**Parameters** + +- **Shift at 5' end** should be used when the location of interest is at a fixed distance from + the 5' end for **all sequenced fragments**! + + For example if the sequenced sample consists + mono-nucleosomal DNA (146bp) we should expect that + each nucleosome midpoint is located at 73 bp from the 5' end of the fragment. + Therefore we would enter 73 as the shift parameter. Once corrected the reads + on each strand will coincide and indicate the actual midpoints + of the nucleosomes. + + When shifting the averaging process in GeneTrack is able correct for longer or shorter + than expected fragment sizes as long as the errors are reasonably random. + +</help> + +</tool>