Mercurial > repos > xuebing > sharplabtool
diff tools/ilmn_pacbio/smrtpipe_hybrid.xml @ 0:9071e359b9a3
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author | xuebing |
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date | Fri, 09 Mar 2012 19:37:19 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/ilmn_pacbio/smrtpipe_hybrid.xml Fri Mar 09 19:37:19 2012 -0500 @@ -0,0 +1,59 @@ +<tool id="smrtpipe_hybrid" name="AHA" version="1.0.0"> + <description>Assemble contigs from a set of contigs and PacBio reads.</description> + <command interpreter="python"> + smrtpipe_galaxy.py --nproc=24 --dat_extension=fasta --output=data/scaffold.fasta --galaxy_output=${outfile} ${iniFile} + </command> + <!-- + <command>cp ${iniFile} ${outfile}</command> + --> + <inputs> + <param name="contigs" format="fasta" type="data" label="Starting Contigs"/> + <param name="reads" format="fasta" type="data" label="PacBio Reads"/> + <param name="schedule" type="text" value="6,3,75;6,3,75;5,3,75;5,3,75;6,2,75;6,2,75;5,2,75;5,2,75" label="Parameter Schedule" size="60"/> + </inputs> + <configfiles> + <configfile name="iniFile"> +[input] +assembled_contigs:${contigs} +file:${reads} + +[HybridAssembly] +instrumentModel=RS +cleanup=False +untangler=pacbio +#set $schedule2 = $schedule.replace('X',';') +paramSchedule=${schedule2} +dontFillin=False +longReadsAsStrobe=True +exactQueryIds=True +rm4Opts=-minMatch 7 -minFrac 0.1 -minPctIdentity 65 -bestn 10 -noSplitSubreads +numberProcesses=16 +cluster=False +minRepeatLength=100000 + </configfile> + </configfiles> + <outputs> + <data name="outfile" format="fasta" label="Hybrid assembly contigs from ${on_string}"/> + </outputs> + <help> + +**What it does** + +The AHA assembly algorithm is an AMOS_-based pipeline +for finishing bacterial-sized +genomes using draft contigs and PacBio reads. + +.. _AMOS: http://sourceforge.net/apps/mediawiki/amos + +**Parameter list** + +Parameter schedule + The parameter schedule is a semi-colon delimited list of triples. Each triple represents an iteration of hybrid assembly (alignment/scaffolding/gap-filling). The three paremeters for each iteration are the Z-score, number of reads required to define a link, and the minimum length of subreads used in links. + +**Output** + +FASTA file containing scaffolded and gap-filled contigs resulting from the +hybrid assembly. + + </help> +</tool>