Mercurial > repos > xuebing > sharplabtool
diff tools/solid_tools/solid_qual_stats.xml @ 0:9071e359b9a3
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author | xuebing |
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date | Fri, 09 Mar 2012 19:37:19 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/solid_tools/solid_qual_stats.xml Fri Mar 09 19:37:19 2012 -0500 @@ -0,0 +1,69 @@ +<tool id="solid_qual_stats" name="Compute quality statistics" version="1.0.0"> + <description>for SOLiD data</description> + <command interpreter="python">solid_qual_stats.py $input $output1</command> + + <inputs> + <param format="qualsolid" name="input" type="data" label="SOLiD qual file" help="If your dataset doesn't show up in the menu, click the pencil icon next to your dataset and set the datatype to 'qualsolid'" /> + </inputs> + <outputs> + <data format="txt" name="output1" metadata_source="input" /> + </outputs> + <tests> + <test> + <param name="input" value="qualscores.qualsolid" /> + <output name="output1" file="qualsolid.stats" /> + </test> + </tests> + +<help> + +**What it does** + +Creates quality statistics report for the given SOLiD quality score file. + +.. class:: infomark + +**TIP:** This statistics report can be used as input for **Quality Boxplot for SOLiD data** tool. + +----- + +**The output file will contain the following fields:** + +* column = column number (position on the read) +* count = number of bases found in this column. +* min = Lowest quality score value found in this column. +* max = Highest quality score value found in this column. +* sum = Sum of quality score values for this column. +* mean = Mean quality score value for this column. +* Q1 = 1st quartile quality score. +* med = Median quality score. +* Q3 = 3rd quartile quality score. +* IQR = Inter-Quartile range (Q3-Q1). +* lW = 'Left-Whisker' value (for boxplotting). +* rW = 'Right-Whisker' value (for boxplotting). + + + + + +**Output Example**:: + + column count min max sum mean Q1 med Q3 IQR lW rW + 1 6362991 2 32 250734117 20.41 5 9 28 23 2 31 + 2 6362991 2 32 250531036 21.37 10 26 30 20 5 31 + 3 6362991 2 34 248722469 19.09 10 26 30 20 5 31 + 4 6362991 2 34 247654797 18.92 10 26 30 20 5 31 + . + . + 32 6362991 2 31 143436943 16.54 3 10 25 22 2 31 + 33 6362991 2 32 114269843 16.96 3 10 25 22 2 31 + 34 6362991 2 29 140638447 12.10 3 10 25 22 2 29 + 35 6362991 2 29 138910532 11.83 3 10 25 22 2 29 + +------ + +This tool is based on `FASTX-toolkit`__ by Assaf Gordon. + + .. __: http://hannonlab.cshl.edu/fastx_toolkit/ +</help> +</tool>