--- a/intersectSig.py	Sat Mar 10 08:17:36 2012 -0500
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,90 +0,0 @@
-'''
-find overlap and test signifiance
-'''
-
-import os,sys
-
-def lineCount(filename):
-    if os.stat(filename).st_size == 0:
-        return 0
-    with open(filename) as f:
-        for i, l in enumerate(f):
-            pass
-            print i
-    return i+1
-
-def intersect(fileA,fileB,outfile,fraction,reciprocal):
-    # return fileA intervals that overlap with interval in fileB
-    cmd = 'intersectBed -a '+fileA+' -b '+fileB + ' -u -wa -f '+fraction +' '+ reciprocal + '>'+outfile
-    #print cmd
-    os.system(cmd)
-    
-def shuffle(fileA,fileB,genomefile,fraction,reciprocal,N):
-    # shuffle fileA N times, return the distribution of overlaps
-    nOverlap = []
-    for i in range(N):
-        # shuffle fileA using shuffleBed
-        #cmd = 'shuffleBed -i '+fileA+' -g '+genomefile +'>fileA.shuffled'
-        # using random_interval.py
-        cmd = 'python /Users/xuebing/galaxy-dist/tools/mytools/random_interval.py '+fileA+' fileA.shuffled across '+genomefile
-        os.system(cmd)
-        intersect('fileA.shuffled',fileB,'tmp',fraction,reciprocal)
-        nOverlap.append(lineCount('tmp'))
-    os.system('rm tmp')
-    os.system('rm fileA.shuffled')
-    return nOverlap
-
-def main():
-    fileA = sys.argv[1]
-    fileB = sys.argv[2]
-    outfile = sys.argv[3]
-    outplot = sys.argv[4]
-    outshuffle = sys.argv[5]
-    N = int(sys.argv[6]) # times to shuffle
-    genomefile = sys.argv[7]
-    fraction = sys.argv[8]
-    if len(sys.argv) == 10:
-        reciprocal = sys.argv[9] # can only be '-r'
-    else:
-        reciprocal = ''
-
-    #print sys.argv
-
-    # number of lines in input
-    nA = lineCount(fileA)
-    nB = lineCount(fileB)    
-
-    # intersect on real data
-    intersect(fileA,fileB,outfile,fraction,reciprocal)
-    # number of overlaps
-    nOverlapReal = lineCount(outfile)
-
-    #print 'number of intervals in inputA that overlap with intervals in inputB:',nOverlapReal
-    
-    # shuffle fileA to estimate background
-    nOverlapNull = shuffle(fileA,fileB,genomefile,fraction,reciprocal,N)
-    out = open(outshuffle,'w')
-    out.write("\t".join(map(str,nOverlapNull)))
-    out.close()
-
-    # plot histogram
-    rscript = open('tmp.r','w')
-    rscript.write("options(warn=-1)\n")
-    rscript.write("x0 <- "+str(nOverlapReal)+"\n")
-    rscript.write("x <- c("+','.join(map(str,nOverlapNull))+")\n")
-    rscript.write("library(MASS)\n")
-    rscript.write("pv <- min((1+sum(x>=x0))/length(x),(1+sum(x<=x0))/length(x))\n")
-    rscript.write("title <- paste('actual:chance = ',x0,':',format(mean(x),digits=1,nsmall=1),' = ',format(x0/mean(x),digits=1,nsmall=2),', p-value < ',pv,sep='')\n")
-    rscript.write("pdf('"+outplot+"')\n")
-    rscript.write("library(grid)\n")
-    rscript.write("library(VennDiagram)\n")
-    rscript.write("venn <- venn.diagram(x=list(A=1:"+str(nA)+",B="+str(nA-nOverlapReal+1)+":"+str(nA+nB-nOverlapReal)+"),filename=NULL,fill=c('red','blue'),col='transparent',alpha=0.5,label.col='black',cex=3,lwd=0,fontfamily='serif',fontface='bold',cat.col = c('red', 'blue'),cat.cex=3,cat.fontfamily='serif',cat.fontface='bold')\n")
-    rscript.write("grid.draw(venn)\n")
-    rscript.write("h <- hist(x,breaks=50,xlab='number of overlaps',ylab='frequency',main=title)\n")
-    rscript.write("plot(h$mids,h$counts,type='h',xlim=c(min(h$mids,x0),max(x0,h$mids)),ylim=c(0,max(h$counts)),xlab='number of overlaps',ylab='frequency',main=title)\n")
-    rscript.write("points(x0,0,col='red')\n")
-    rscript.write("dev.off()\n")
-    rscript.close()
-    os.system("R --vanilla < tmp.r")    
-    os.system('rm tmp.r')
-main()